Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,680,1230AT35.7% 9.4 / 10.7 14intergenic (+175/+357)cbpD/USA300HOU_2537possible secretory antigen/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (4/5);  new base T (2/3);  total (6/8)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.24e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

TTTTATGAAATTATATTATTACTTCTACAAATTTCAAATTGCCGTAATTGAACGTATATTTCTTCTTCAACTATTATTTCATCTTTAGCATAATCTATATATAAAATTTTATGCTATTATTTAAATAATTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTCAATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAAATAATTTAACTGAGTTTTTGAGACCTGTTTAAATTGAT  >  CP000730/2680008‑2680262
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ttttatGAAATTATATTATTACTTCTACAAATTTCAAATTGCCGTAATTGAACGTATATTTCTTCTTCAACTATTATTTCATCTTTAGCATAATCTATATATAAAATTTTATGCTATTATTTAAATAATTCGCTATAACtt                                                                                                                       >  1:325578/1‑141 (MQ=255)
   tatGAAATTATATTATTACTTCTACAAATTTCAAATTGCCGTAATTGAACGTATATTTCTTCTTCAACTATTATTTCATCTTTAGCATAATCTATATATAAAATTTTATGCTATTATTTAAATAATTCGCTATAACTTAAc                                                                                                                    >  1:235264/1‑141 (MQ=255)
    atGAAATTATATTATTACTTCTACAAATTTCAAATTGCCGTAATTGAACGTATATTTCTTCTTCAACTATTATTTCATCTTTAGCATAATCTATATATAAAATTTTATGCTATTATTTAAATAATTCGCTATAACTTAACa                                                                                                                   <  2:97234/141‑1 (MQ=255)
                           cAAATTTCAAATTGCCGTAATTGAACGTATATTTCTTCTTCAACTATTATTTCATCTTTAGCATAATCTATATATAAAATTTTATGCTATTATTTAAATAATTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGt                                                                                            <  2:73048/141‑1 (MQ=255)
                                               ttGAACGTATATTTCTTCTTCAACTATTATTTCATCTTTAGCATAATCTATATATAAAATTTTATGCTATTATTTAAATAATTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTCAATAAttttt                                                                       >  2:43527/1‑141 (MQ=255)
                                                      tataTTTCTTCTTCAACTATTATTTCATCTTTAGCATAATCTATATATAAAATTTTATGCTATTATTTAAATAATTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTCAATAATTTTTTGCTGtt                                                              <  1:61565/141‑1 (MQ=255)
                                                                            attcctatctatcATAAGCTATATATAATATTTTATGGTAATAGTGAAATAATACGGTATAATTTAACGTACGCTTTCGATGTAAACCTTGTTCTAAATCTCAATAATTTTTTGCTGTTTTCATCGTCAATAGTAaaaaaa                                        <  2:312704/129‑1 (MQ=255)
                                                                               cATCTTTAGCATAATCTATATATAAAATTTTATGCTATTATTTAAATAATTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTCAATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAaata                                     >  1:20193/1‑141 (MQ=255)
                                                                                                 tatataAAATTTTATGCTATTATTTAAATAATTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTCAATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAAATAATTTAACTGAGTTTTTga                   <  1:192526/141‑1 (MQ=255)
                                                                                                   tttaAAATTTTATGTTTTTGTTTAAGCAATCCACCATAACTTAACATACGTTTTCGATACAAACCttttt                                                                                           >  2:215592/3‑67 (MQ=255)
                                                                                                   tttaAAATTTTATGTTTTTGTTTAAGCAATCCACCATAACTTAACATACGTTTTCGATACAAACCttttt                                                                                           <  1:215592/68‑4 (MQ=255)
                                                                                                     taAAATTTTATGTTTTTGTTTAAGCAATCCACCATAACTTAACATACGTTTTCGATACAAACCTTTTTCTAAATCACTTACAATTTCTTGATTTTTTTCATCATCATCAGTTaaaaaatcag                                    <  2:69854/122‑5 (MQ=255)
                                                                                                      aaaaTTTTATGTTTTTGTTTAAGCAATCCACCATAACTTAACATACGTTTTCGATACAAACCTTTTTCTAAATCACTTACAATTTCTTGATTTTTTTCATCATCATCAGTTaaaaaatcaga                                   >  2:192923/1‑117 (MQ=255)
                                                                                                      aaaaTTTTATGTTTTTGTTTAAGCAATCCACCATAACTTAACATACGTTTTCGATACAAACCTTTTTCTAAATCACTTACAATTTCTTGATTTTTTTCATCATCATCAGTTaaaaaatcaga                                   <  1:192923/122‑6 (MQ=255)
                                                                                                                  tattatTTAAATAATTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTCAATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAAATAATTTAACTGAGTTTTTGAGCCCTGTTTAAATtgat  >  1:282684/1‑141 (MQ=255)
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TTTTATGAAATTATATTATTACTTCTACAAATTTCAAATTGCCGTAATTGAACGTATATTTCTTCTTCAACTATTATTTCATCTTTAGCATAATCTATATATAAAATTTTATGCTATTATTTAAATAATTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTCAATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAAATAATTTAACTGAGTTTTTGAGACCTGTTTAAATTGAT  >  CP000730/2680008‑2680262

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: