Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	9,280	G→A	R750H (CGT→CAT)	gyrA →	DNA topoisomerase (ATP‑hydrolyzing) subunit A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	9,280	0	G	A	100.0%	120.9 / NA	35	R750H (CGT→CAT)	gyrA	DNA topoisomerase (ATP‑hydrolyzing) subunit A
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/12);  total (23/12)

GCTCATGCAAACAGTGTTGATGAAGTATTAGTAGTTACTGAAAATGGTTATGGTAAACGTACGCCAGTTAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCGTAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGGTGTCATTATTCGACTAGATGTTGCAGATATTTCTCAAAATGGTCGTGCAGCACAAGGTGTTCG  >  CP000730/9150‑9412                                                                                                                                   |                                                                                                                                     gCTCATGCAAACAGTGTTGATGAAGTATTAGTAGTTACTGAAAATGGTTATGGTAAACGTACGCCAGTTAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCataatggtaat                                                                                                                            >  2:211352/1‑141 (MQ=255)  cTCATGCAAACAGTGTTGATGAAGTATTAGTAGTTACTGAAAATGGTTATGGTAAACGTACGCCAGTTAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCataatggtaatg                                                                                                                           >  2:59649/1‑141 (MQ=255)        cAAACAGTGTTGATGAAGTATTAGTAGTTACTGAAAATGGTTATGGTAAACGTACGCCAGTTAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTtgtat                                                                                                                     <  1:296044/141‑1 (MQ=255)        cAAACAGTGTTGATGAAGTATTAGTAGTTACTGAAAATGGTTATGGTAAACGTACGCCAGTTAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTtgtat                                                                                                                     <  2:177863/141‑1 (MQ=255)              gtgtTGATGAAGTATTAGTAGTTACTGAAAATGGTTATGGTAAACGTACGCCAGTTAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCa                                                                                                               >  1:242292/1‑141 (MQ=255)              gtgtTGATGAAGTATTAGTAGTTACTGAAAATGGTTATGGTAAACGTACGCCAGTTAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATCTTGTATGTATCa                                                                                                               >  2:204762/1‑141 (MQ=255)                  tgaagaAGTATTAGTATTTACTGAAAAAGGTTATGGAAAAAGTACACCAGTTAATGACTATCGAATAAAAAATCGAGGTGCGAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTCGATGTATCACTAc                                                                                                           >  2:220448/1‑141 (MQ=255)                      gaAGTATTAGTAGTTACTGAAAATGGTTATGGTAAACGTACGCCAGTTAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTa                                                                                                       >  1:263249/1‑141 (MQ=255)                                 agtTACTGAAAATGGTTATGGTAAACGTACGCCAGTTAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTgaaga                                                                                            >  1:122577/1‑141 (MQ=255)                                      cTGAAAATGGTTATGGTAAACGTACGCCAGTTAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGAtt                                                                                       >  2:33582/1‑141 (MQ=255)                                            aTGGTTATGGTAAACGTACGCCAGTTAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGa                                                                                 >  1:192219/1‑141 (MQ=255)                                            aTGGTTATGGTAAACGTACGCCAGTTAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGa                                                                                 <  1:3834/141‑1 (MQ=255)                                                ttATGGTAAACGTACGCCAGTTAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGt                                                                             >  1:150814/1‑141 (MQ=255)                                                ttATGGTAAACGTACGCCAGTTAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGt                                                                             >  2:75907/1‑141 (MQ=255)                                                         aCGTACGCCAGTTAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGc                                                                    <  2:111518/141‑1 (MQ=255)                                                           gTACGCCAGTTAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAg                                                                  >  1:283884/1‑141 (MQ=255)                                                               gCCAGTTAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGgtgt                                                              >  1:148644/1‑141 (MQ=255)                                                                ccAGTTAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGGTGTc                                                             >  2:232673/1‑141 (MQ=255)                                                                  aGTTACTGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTACACCAGATCCGATAACCGAGCACATAGGAAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGGTGTCat                                                           <  1:207788/141‑1 (MQ=255)                                                                                   tATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGGTGTCATTATTCGACTAGATGTTg                                          >  1:288956/1‑141 (MQ=255)                                                                                       aaaTCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGGTGTCATTATTCGACTAGATGTTGCAGa                                      <  2:139162/141‑1 (MQ=255)                                                                                         aTCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGGTGTCATTATTCGACTAGATGTTGCAGata                                    <  2:197441/141‑1 (MQ=255)                                                                                          tCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGGTGTCATTATTCGACTAGATGTTGCAGatat                                   <  1:33582/141‑1 (MQ=255)                                                                                              ggtagtAAAGGTATTAAAACAGCTACGATTGCTGAGCATAATGATAATGTTGTATGTATCACGACAGTAACTGGTTAATAAGATTTAATGATGGTGACTAATTCAAGTGTCATTATTCGACTAGATGTTGCAGATATTtct                               <  2:166212/141‑1 (MQ=255)                                                                                               gtggtAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGGTGTCATTATTCGACTAGATGTTGCAGATATTtctc                              >  2:144465/1‑141 (MQ=255)                                                                                                  gtAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGGTGTCATTATTCGACTAGATGTTg                                          >  2:79312/1‑126 (MQ=255)                                                                                                  gtAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGGTGTCATTATTCGACTAGATGTTg                                          <  1:79312/126‑1 (MQ=255)                                                                                                           ttAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGGTGTCATTATTCGACTAGATGTTGCAGATATTTCTCAAAATGGTCGTg                  >  2:160060/1‑141 (MQ=255)                                                                                                           ttAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGGTGTCATTATTCGACTAGATGTTGCAGATATTTCTCAAAATGGTCGTg                  <  2:148644/141‑1 (MQ=255)                                                                                                           ttAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGGTGTCATTATTCGACTAGATGTTGCAGATATTTCTCAAAATGGTCGTg                  >  1:125419/1‑141 (MQ=255)                                                                                                                 cAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGGTGTCATTATTCGACTAGATGTTGCAGATATTTCTCAAAATGGTCGTgccgcaa            >  2:315465/1‑140 (MQ=255)                                                                                                                 cAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGGTGTCATTATTCGACTAGATGTTGCAGATATTTCTCAAAATGGTCGTGCAGcac            >  1:59213/1‑141 (MQ=255)                                                                                                                    cTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGGTGTCATTATTCGACTAGATGTTGCAGATATTTCTCAAAATGGTCGTGCAGCACAAg         >  1:56405/1‑141 (MQ=255)                                                                                                                     tACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGGTGTCATTATTCGACTAGATGTTGCAGATATTTCTCAAAATGGTCGTGCAGCACAAgg        >  2:220702/1‑141 (MQ=255)                                                                                                                         aTTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGGTGTCATTATTCGACTAGATGTTGCAGATATTTCTCAAAATGGTCGTGCAGCACAAGGTGtt    >  1:200960/1‑141 (MQ=255)                                                                                                                          ttACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGGTGTCATTATTCGACTAGATGTTGCAGATATTTCTCAAAATGGTCGTGCAGCACAAGGTGTTc   <  2:150814/141‑1 (MQ=255)                                                                                                                           tACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGGTGTCATTATTCGACTAGATGTTGCAGATATTTCTCAAAATGGTCGTGCAGCACAAGGTGTTCg  >  1:244010/1‑141 (MQ=255)                                                                                                                                   |                                                                                                                                     GCTCATGCAAACAGTGTTGATGAAGTATTAGTAGTTACTGAAAATGGTTATGGTAAACGTACGCCAGTTAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCGTAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGGTGTCATTATTCGACTAGATGTTGCAGATATTTCTCAAAATGGTCGTGCAGCACAAGGTGTTCG  >  CP000730/9150‑9412

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑