| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP000730 | 1,576,651 | A→G | 23.6% | F289S (TTC→TCC) | hlgB ← | possible bacteriophage tail protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 1,576,651 | 0 | A | G | 23.6% | 23.4 / 6.7 | 17 | F289S (TTC→TCC) | hlgB | possible bacteriophage tail protein |
| Reads supporting (aligned to +/- strand): ref base A (6/7); new base G (2/2); total (8/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.65e-01 | |||||||||||
TTAATGCTGCACCTAACCCTAAAGTAATTGGTGTAGATA‑CGCCCATCGTCATTGTACGTCCCAGGGAAGTCATTTTGTCTCCAATAGAACTAAATTTCTTTGACATGACATCCGCTTGACTTGCAAGTTTACCGAAATGACTTTGAGCTATCATTTGTTCTTTGTTAAAAGTCTTCATTTCGGATGAAGCTTTATCTATTGAACGCTCCAAATTATTTAAAGCAGCTTTTTCTTTATTAACAGCTGTTTCAGCTTTTGCGACATTAGCGC > CP000730/1576517‑1576786 | ttAATGCTGCACCTAACCCTAAAGTAATTGGTGTAGATA‑CGCCCATCGTCATTGTACGTCCCAGGGAAGTCATTTTGTCTCCAATAGAACTAAATTTCTTTGACATGACATCCGCTTGACTTGCAAGTTTACCGAAATGAc < 2:121017/141‑1 (MQ=255) gcACCTAACCCTAAAGTAATTGGTGTAGATATCGGTGGTCG‑CCGTATAATTAAAAAAGAAATCATTTTGTCTCCAATAGAACTAAATTTCTTTGAAATGACATCCGCTTGACTTGCAAGTTTACCGAAATAACtttatgct > 2:3162/1‑136 (MQ=255) gcACCTAACCCTAAAGTAATTGGTGTAGATA‑CGCCCATCGTCATTGTACGTCCCAGGGAAGTCATTTTGTCTCCAATAGAACTAAATTTCTTTGACATGACATCCGCTTGACTTGCAAGTTTACCGAAATGACTTTGAGCt > 1:167177/1‑141 (MQ=255) aGTAATTGGTGTAGATA‑CGCCCATCGTCATTGTACGTCCCAGGGAAGTCATTTTGTCTCCAATAGAACTAAATTTCTTTGACATGACATCCGCTTGACTTGCAAGTTTACCGAAATGACTTTGAGCTATCATTTGTTCttt < 2:250157/141‑1 (MQ=255) gTCATTGTACGTCCCAGGGAAGTCATTTTGTCTCCAATAGAACTAAATTTCTTTGACATGACATCCGCTTGACTTGCAAGTTTACCGAAATGACTTTGAGCTATCATTTGTTCTTTGTTAAAAGTCTTCATTTCGGATGaa > 1:15662/1‑141 (MQ=255) gTCATTGTACGTCCCAGGGAAGTCATTTTGTCTCCAATAGAACTAAATTTCTTTGACATGACATCCGCTTGACTTGCAAGTTTACCGAAATGACTTTGAGCTATCATTTGTTCTTTGTTAAAAGTCTTCATTTCGGATGaa > 1:15775/1‑141 (MQ=255) cATTGTACGTCCCAGGGAAGTCATTTTGTCTCCAATAGAACTAAATTTCTTTGACATGACATCCGCTTGACTTGCAAGTTTACCGAAATGACTTTGAGCTATCATTTGTTCTTTGTTAAAAGTCTTCATTTCGGATGAAGc > 1:221843/1‑141 (MQ=255) aaGTCATTTTGTCTCCAATAGAACTAAATTTCTTTGACATGACATCCGCTTGACTTGCAAGTTTACCGGAATGACTTTGAGCTATCATTTGTTCTTTGTTAAAAGTCTTCATTTCGGATGAAGCTTTATCTATTGAACGCt > 1:98996/1‑141 (MQ=255) aaGTCATTTTGTCTCCAATAGAACTAAATTTCTTTGACATGACATCCGCTTGACTTGCAAGTTTACCGGAATGACTTTGAGCTATCATTTGTTCTTTGTTAAAAGTCTTCATTTCGGATGAAGCTTTATCTATTGAACGCt > 1:99073/1‑141 (MQ=255) aTAGAACTAAATTTCTTTGACATGACATCCGCTTGACTTGCAAGTTTACCGAAATGACTTTGAGCTATCATTTGTTCTTTGTTAAAAGTCTTCATTTCGGATGAAGCTTTATCTATTGAACGCTCCAAATTATTTAAagca < 2:221843/141‑1 (MQ=255) aGAACTAAATTTCTTTGACATGACATCCGCTTGACTTGCAAGTTTACCGAAATGACTTTGAGCTATCATTTGTTCTTTGTTAAAAGTCTTCATTTCGGATGAAGCTTTATCTATTGAACGCTCCAAATTATTTAAagcagc < 1:30597/141‑1 (MQ=255) aaTTTCTTTGACATGACATCCGCTTGACTTGCAAGTTTACCGAAATGACTTTGAGCTATCATTTGTTCTTTGTTAAAAGTCTTCATTTCGGATGAAGCTTTATCTATTGAACGCTCCAAATTATTTAAAGCAGCTTTTTCt < 1:47955/141‑1 (MQ=255) aaTTTCTTTGACATGACATCCGCTTGACTTGCAAGTTTACCGAAATGACTTTGAGCTATCATTTGTTCTTTGTTAAAAGTCTTCATTTCGGATGAAGCTTTATCTATTGAACGCTCCAAATTATTTAAAGCAGCTTTTTCt < 1:60173/141‑1 (MQ=255) tttGACATGACATCCACTTGACTTGCAAGTTTACCGAAATGACTTTGAGCTATCATTTGTTCTTTGTTAAAAGTCTTCATTTCGGATGAAGCTTTATCTATTGAACGCTCCAAATTATTTAAAGCAGCTTTTTCTttatta > 2:79757/1‑141 (MQ=255) cTTGCAAGTTTACCGAAATGACTTTGAGCTATCATTTGTTCTTTGTTAAAAGTCTTCATTTCGGATGAAGCTTTATCTATTGAACGCTCCAAATTATTTAAAGCAGCTTTTTCTTTATTAACAGCTGTTTCAGCTTTTGCg < 2:31946/141‑1 (MQ=255) tACCGGAATGACTTTGAGCTATCATTTGTTCTTTGTTAAAAGTCTTCATTTCGGATGAAGCTTTATCTATTGAACGCTCCAAATTATTTAAAGCAGCTTTTTCTTTATTAACAGCTGTTTCAGCTTTTGCGACATTAgcgc < 2:98996/141‑1 (MQ=255) tACCGGAATGACTTTGAGCTATCATTTGTTCTTTGTTAAAAGTCTTCATTTCGGATGAAGCTTTATCTATTGAACGCTCCAAATTATTTAAAGCAGCTTTTTCTTTATTAACAGCTGTTTCAGCTTTTGCGACATTAgcgc < 2:99073/141‑1 (MQ=255) | TTAATGCTGCACCTAACCCTAAAGTAATTGGTGTAGATA‑CGCCCATCGTCATTGTACGTCCCAGGGAAGTCATTTTGTCTCCAATAGAACTAAATTTCTTTGACATGACATCCGCTTGACTTGCAAGTTTACCGAAATGACTTTGAGCTATCATTTGTTCTTTGTTAAAAGTCTTCATTTCGGATGAAGCTTTATCTATTGAACGCTCCAAATTATTTAAAGCAGCTTTTTCTTTATTAACAGCTGTTTCAGCTTTTGCGACATTAGCGC > CP000730/1576517‑1576786 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |