Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	2,451,070	T→C	9.8%	K116K (AAA→AAG)	hutU ←	urocanate hydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,451,070	0	T	C	9.8%	110.2 / 5.6	41	K116K (AAA→AAG)	hutU	urocanate hydratase
Reads supporting (aligned to +/- strand):  ref base T (22/15);  new base C (2/2);  total (24/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.68e-01

AACAGTGCCTGCTAAATCTCCATTAAAGTGTTGATTACCTAGCTCGGCAAATGTCTCATAAGTACCTTGCACAATACCTTGAGAACCAATATATATCCAACTACCGGCTGTCATCTGTCCATACATGATTAAACCTTTTTTATCTAATTCATTAAAATGATCCCAGTTTGCCCATTCAGGCACTAATACTGAATTTGAAATTAATACACGTGGCGCTTCTTCATGTGTTTTAAATACAGCAACTGGCTTTCCTGATTGTACTAACAT  >  CP000730/2450935‑2451201                                                                                                                                        |                                                                                                                                    aaCAGTGCCTGCTAAATCTCCATTAAAGTGTTGATTACCTAGCTCGGCAAATGTCTCATAAGTACCTTGCACAATACCTTGAGAACCAATATATATCCAACTACCGGCTGTCATCTGTCCATACATGATTAAACCtttttt                                                                                                                                <  1:226704/141‑1 (MQ=255) aaCAGTGCCTGCTAAACCGACAGTAAAGTGTTGATTCCCTAGCTCGGCAAATGTCTCATAAGTACCTTGCACAATACCTTGAGAACCAATATATATCCAACTACCGGCTGTCATCTGTCCATACATGATTAAACCtttttt                                                                                                                                <  1:350429/141‑1 (MQ=255)        ccTGCTAAATCTCCATTAAAGTGTTGATTACCTAGCTCGGCAAATGTCTCATAAGTACCTTGCACAATACCTTGAGAACCAATATATATCCAACTACCGGCTGTCATCTGTCCATACATGATTAAACCTTTTTTATCTAAt                                                                                                                         >  2:427464/1‑141 (MQ=255)        ccTGCTAAATCTCCATTAAAGTGTTGATTACCTAGCTCGGCAAATGTCTCATAAGTACCTTGCACAATACCTTGAGAACCAATATATATCCAACTACCGGCTGTCATCTGTCCATACATGATTAAACCTTTTTTATCTAAt                                                                                                                         >  2:136006/1‑141 (MQ=255)        ccTGCTAAATCTCCATTAAAGTGTTGATTACCTAGCTCGGCAAATGTCTCATAAGTACCTTGCACAATACCTTGAGAACCAATATATATCCAACTACCGGCTGTCATCTGTCCATACATGATTAAACCCTTTTTATCTAAt                                                                                                                         >  1:347372/1‑141 (MQ=255)        ccTGCTAAATCTCCATTAAAGTGTTGATTACCTAGCTAGGCAAATGTCTCATAAGTACCTTGCACAATACCTTGAGAACCAATATATATCCAACTACCGGCTGTCATCTGTCCATACATGATTAAACCCTTTTTATCTAAt                                                                                                                         >  1:196373/1‑141 (MQ=255)        ccTGCTAAATCTCCAGGAAATTGTTGATTACCTAGCTCGGCAAATGTCTCATAAGTACCTTGCACAATACCTTGAGAACCAATATATATCCAACTACCGGCTGTCATCTGTCCATACATGATTAAACCTTTTTTATCTAAt                                                                                                                         >  2:94634/1‑141 (MQ=255)         cTGCTAAATCTCCATTAAAGTGTTGATTACCTAGCTCGGCAAATGTCTCATAAGTACCTTGCACAATACCTTGAGAACCAATATATATCCAACTACCGGCTGTCATCTGTCCATACATGATTAAACCTTTTTTATCTAAtt                                                                                                                        <  2:13815/141‑1 (MQ=255)             tAAATCTCCATTAAAGTGTTGATTACCTAGCTCGGCAAATGTCTCATAAGTACCTTGCACAATACCTTGAGAACCAATATATATCCAACTACCGGCTGTCATCTGTCCATACATGATTAAACCTTTTTTATCTAATTCAtt                                                                                                                    <  2:303746/141‑1 (MQ=255)             tAAATCTCCATTAAAGTGTTGATTACCTAGCTCGCCAAATGTCTCATAAGTACCTTGCACAATACCTTGAGAACCAATATATATCCAACTACCGGCTGTCATCTGTCCATACATGATTAAACCTTTTTTATCTAATTCAtt                                                                                                                    <  1:426414/141‑1 (MQ=255)                    ccATTAAAGTGTTGATTACCTAGCTCGGCAAATGTCTCATAAGTACCTTGCACAATACCTTGAGAACCAATATATATCCAACTACCGGCTGTCATCTGTCCATACATGATTAAACCTTTTTTATCTAATTCATTAAAATGa                                                                                                             <  2:259318/141‑1 (MQ=255)                     cATTAAAGTGTTGATTACCTAGCTCGGCAAATGTCTCATAAGTACCTTGCACAATACCTTGAGAACCAATATATATCCAACTACCGGCTGTCATCTGTCCATACATGATTAAACCTTTTTTATCTAATTCATTAAAATGAt                                                                                                            >  2:194906/1‑141 (MQ=255)                     cATTAAAGTGTTGATTACCTAGCTCGGCAAATGTCTCATAAGTACCTTGCACAATACCTTGAGAACCAATATATATCCAACTACCGGCTGTCATCTGTCCATACATGATTAAACCTTTTTTATCTAATTCATTAAAATGAt                                                                                                            >  2:291869/1‑141 (MQ=255)                       ttAAAGTGTTGATTACCTAGCTCGGCAAATGTCTCATAAGTACCTTGCACAATACCTTGATAACCAATATATATCCATCTACCGGCTGTCATCTGTCCATACATGATTAAACCTTTTTTATCTAATTCATTAAAATGATcc                                                                                                          <  1:230041/141‑1 (MQ=255)                       ttAAAGTGTTGATTACCTAGCTCGGCAAATGTCTCATAAGTACCTTGCACAATACCTTGAGAACCAATATATATCCAACTACCGGCTGTCATCTGTCCATACATGATTAAACCTTTTTTATCTAATTCATTAAAATGATcc                                                                                                          <  1:290244/141‑1 (MQ=255)                                 gATTACCTAGCTCGGCAAATGTCTCATAAGTACCTTGCACAATACCTTGAGAACCAATATATATCCAACTACCGGCTGTCATCTGTCCATACATGATTAAACCTTTTTTATCTAATTCATTAAAATGATCCCAGTTTGccc                                                                                                >  2:194417/1‑141 (MQ=255)                                     aCCTAGCTCGGCAAATGTCTCATAAGTACCTTGCACAATACCTTGAGAACCAATATATATCCAACTACCGGCTGTCATCTGTCCATACATGATTAAACCTTTTTTATCTAATTCATTAAAATGATCCCAGTTTGCCCATTc                                                                                            >  1:96245/1‑141 (MQ=255)                                                cAAATGTCTCATAAGTACCTTGCACAATACCTTGAGAACCAATATATATCCAACTACCGGCTGTCATCTGTCCATACATGATTAAACCTTTTTTATCTAATTCATTAAAATGATCCCAGTTTGCCCATTCAGGCACTAATa                                                                                 <  1:444568/141‑1 (MQ=255)                                                cAAATGTCTCATAAGTACCTTGCACAATACCTTGAGAACCAATATATATCCAACTACCGGCTGTCATCTGTCCATACATGATTAAACCTTTTTTATCTAATTCATTAAAATGATCCCAGTTTGCCCATTCAGGCACTAATa                                                                                 <  1:324862/141‑1 (MQ=255)                                                cAAATGTCTCATAAGTACCTTGCACAATACCTTGAGAACCAATATATATCCAACTACCGGCTGTCATCTGTCCATACATGATTAAACCTTTTTTATCTAATTCATTAAAATGATCCCAGTTTGCCCATTCAGGCACTAATa                                                                                 <  1:94720/141‑1 (MQ=255)                                                     gTCTCATAAGTACCTTGCACAATACCTTGAGAACCAATATATATCCAACTACCGGCTGTCATCTGTCCATACATGATTAAACCTTTTTTATCTAATTCATTAAAATGATCCCAGTTTGCCCATTCAGGCACTAATACTGaa                                                                            >  1:328/1‑141 (MQ=255)                                                        tcATAAGTACCTTGCACAATACCTTGAGAACCAATATATATCCAACTACCGGCTGTCATCTGTCCATACATGATTAAACCTTTTTTATCTAATTCATTAAAATGATCCCAGGTTGCCCATTCAGGCACTAATACTGAAttt                                                                         >  1:454496/1‑141 (MQ=255)                                                         cATAAGTACCTTGCACAATACCTTGAGAACCAATATATATCCAACTACCGGCTGTCATCTGTCCATACATGATTAAACCTTTTTTATCTAATTCATTAAAATGATCCCAGTTTGCCCATTCAGGCACTAATACTGAATTTg                                                                        <  2:25359/141‑1 (MQ=255)                                                            aaGTACCTTGCACAATACCTTGAGAACCAATATATATCCAACTACCGGCTGTCATCTGTCCATACATGATTAAACCTTTTTTATCTAATTCATTAAAATGATCCCAGTTTGCCCATTCAGGCACTAATACTGAATTTGaaa                                                                     >  2:316024/1‑141 (MQ=255)                                                            aaGTACCTTGCACAATACCTTGAGAACCAATATATATCCAACTACCGGCTGTCATCTGTCCATACATGATTAAACCTTTTTTATCTAATTCATTAAAATGATCCCAGTTTGCCCATTCAGGCACTAATACTGAATTTGaaa                                                                     >  2:334129/1‑141 (MQ=255)                                                                            aCCTTGAGAACCAATATATATCCAACTACCGGCTGTCATCTGTCCATACATGATTAAACCTTTTTTATCTAATTCATTAAAATGATCCCAGTTTGCCCATTCAGGCACTAATACTGAATTTGAAATTAATACACGTGgcgc                                                     >  1:393911/1‑141 (MQ=255)                                                                            aCCTTGAGAACCAATATATATCCAACTACCGGCTGACATCTGTCCATACATGATTAAACCTTTTTTATCTAATTCATTAAAATGATCCCAGTTTGCCCATTCAGGCACTAATACTGAATTTGAAATTAATACACGTGgcgc                                                     >  1:401471/1‑141 (MQ=255)                                                                                    aaCCAATATATATCCAACTACCGGCTGTCATCTGTCCATACATGATTAAACCTTTTTTATCTAATTCATTAAAATGATCCCAGTTTGCCCATTCAGGCACTAATACTGAATTTGAAATTAATACACGTGGCGCTTCTTCAt                                             <  1:201562/141‑1 (MQ=255)                                                                                     aCCAATATATATCCAACTACCGGCTGTCATCTGTCCATACATGATTAAACCTTTTTTATCTAATTCATTAAAATGATCCCAGTTTGCCCATTCAGGCACTAATACTGAATTTGAAATTAATACACGTGGCGCTTCTTCAtg                                            <  1:238829/141‑1 (MQ=255)                                                                                                   aaCTACCGGCTGTCATCTGTCCATACATGATTAAACCTTTTTTATCTAATTCATTAAAATGATCCCAGTTTGCCCATTCAGGCACTAATACTGAATTTGAAATTAATACACGTGGCGCTTCTTCATGTGTTTTAAATACAg                              >  1:202666/1‑141 (MQ=255)                                                                                                   aaCTACCGGCTGTCATCTGTCCATACATGATTAAACCTTTTTTATCTAATTCATTAAAATGATCCCAGTTTGCCCATTCAGGCACTAATACTGAATTTGAAATTAATACACGTGGCGCTTCTTCATGTGTTTTAAATACAg                              >  1:4698/1‑141 (MQ=255)                                                                                                        ccGGCTGTCATCTGTCCATACATGATTAAACCTTTTTTATCTAATTCATTAAAATGATCCCAGTTTGCCCATTCAGGCACTAATACTGAATTTGAAATTAATACACGTGGCGCTTCTTCATGTGTTTTAAATACAGCAACt                         >  2:108592/1‑141 (MQ=255)                                                                                                        ccGGCTGTCATCTGTCCATACATGATTAAACCTTTTTTATCTAATTCATTAAAATGATCCCAGTTTGCCCATTCAGGCACTAATACTGAATTTGAAATTAATACACGTGGCGCTTCTTCATGTGTTTTAAATACAGCAACt                         >  1:281487/1‑141 (MQ=255)                                                                                                        ccGGCTGTCATCTGTCCATACATGATTAAACCTTTTTTATCTAATTCATTAAAATGATCCCAGTTTGCCCATTCAGGCACTAATACTGAATTTGAAATTAATACACGTGGCGCTTCTTCATGTGTTTTAAATACAGCAACt                         >  2:48551/1‑141 (MQ=255)                                                                                                        ccGGCTGTCATCTGTCCATACATGATTAAACCTTTTTTATCTAATTCATTAAAATGATCCCAGTTTGCCCATTAAGGCACTAATACTGAATTTGAAATTAATCCACGTGGAGATTCCTCATGTGTTTTAAATACAGCACCt                         >  1:20311/1‑141 (MQ=255)                                                                                                         cGGCTGTCATCTGTCCATACATGATTAAACCTTTTTTATCTAATTCATTAAAATGATCCCAGTTTGCCCATTCAGGCACTAATACTGAATTTGAAATTAATACACGTGGCGCTTCTTCATGTGTTTTAAATACAGCAACTg                        >  2:122955/1‑141 (MQ=255)                                                                                                         cGGCTGTCATCTGTCCATACATGATTAAACCTTTTTTATCTAATTCATTAAAATGATCCCAGTTTGCCCATTCAGGCACTAATACTGAATTTGAAATTAATACACGTGGCGCTTCTTCATGTGTTTTAAATACAGCAACTg                        >  2:9312/1‑141 (MQ=255)                                                                                                                          tACATGATTAAACCTTTTTTATCTAATTCATTAAAATGATCCCAGTTTGCCCATTCAGGCACTAATACTGAATTTGAAATTAATACACGTGGCGCTTCTTCATGTGTTTTAAATACAGCAACTGGCTTTCCTGATTGTACt       <  1:136006/141‑1 (MQ=255)                                                                                                                          tACATGATTAAACCTTTTTTATCTAATTCATTAAAATGATCCCAGTTTGCCCATTCAGGCACTAATACTGAATTTGAAATTAATACACGTGGCGCTTCTTCATGTGTTTTAAATACAGCAACTGGCTTTCCTGATTGTACt       <  1:427464/141‑1 (MQ=255)                                                                                                                          tACATGATTAAACCTTTTTTATCTAATTCATTAAAATGATCCCAGTTTGCCCATTCAGGCACTAATACTGAATTTGAAATTAATACACGTGGCGCTTCTTCATGTGTTTTAAATACAGCAACTGGCTTTCCTGATTGTACt       <  1:94634/141‑1 (MQ=255)                                                                                                                          tACATGATTAAACCCTTTTTATCTAATTCATTAAAATGATCCCAGTTTGCCCATTCAGGCACTAATACTGAATTTGAAATTAATACACGTGGCGCTTCTTCATGTGTTTTAAATACAGCAACTGGCTTTCCTGATTGTACt       <  2:347372/141‑1 (MQ=255)                                                                                                                          tACATGATTAAACCCTTTTTATCTAATTCATTAAAATGATCCCAGTTTGCCCATTCAGGCACTAATACTGAATTTGAAATTAATACACGTGGCGCTTCTTCATGTGTTTTAAATACAGCAACTGGCTTTCCTGATTGTACt       <  2:196373/141‑1 (MQ=255)                                                                                                                               gATTAAACCTTTTTTATCTAATTCATTAAAATGATCCCAGTTTGCCCATTCAGGCACTAATACTGAATTTGAAATTAATACACGTGGCGCTTCTTCATGTGTTTTAAATACAGCAACTGGCTTTCCTGATTGTACTAACAt  >  2:293681/1‑141 (MQ=255)                                                                                                                                        |                                                                                                                                    AACAGTGCCTGCTAAATCTCCATTAAAGTGTTGATTACCTAGCTCGGCAAATGTCTCATAAGTACCTTGCACAATACCTTGAGAACCAATATATATCCAACTACCGGCTGTCATCTGTCCATACATGATTAAACCTTTTTTATCTAATTCATTAAAATGATCCCAGTTTGCCCATTCAGGCACTAATACTGAATTTGAAATTAATACACGTGGCGCTTCTTCATGTGTTTTAAATACAGCAACTGGCTTTCCTGATTGTACTAACAT  >  CP000730/2450935‑2451201

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑