| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP000730 | 1,221,684 | C→A | 19.6% | intergenic (+99/‑111) | rpoZ → / → rpoZ | DNA‑directed RNA polymerase subunit omega/bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate‑‑cysteine ligase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 1,221,684 | 0 | C | A | 19.6% | 50.3 / 7.2 | 22 | intergenic (+99/‑111) | rpoZ/rpoZ | DNA‑directed RNA polymerase subunit omega/bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate‑‑cysteine ligase |
| Reads supporting (aligned to +/- strand): ref base C (10/6); new base A (2/2); total (14/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.05e-01 | |||||||||||
GCCCTGTTATTTCAAGTGATTATTATGGTAAAGAATAGTTTCGACCATTAAAAATATGTGAAGCTAGACGTACATCAAATACGTCAAGGCATATCCAAATCAAACGCACCTATGTAGGTGTGTTTTTTTAGTTTATCCAATGATTGATGTTATAATAATACTAAATTTGTATCTATAAAAAAGTAATGAGCATTTGTGCGCATATGATGATGTAAAGCGTAAATGTCATAGTGATATTTAAGGGAGATTGAATATGAAGAA > CP000730/1221548‑1221808 | gCCCTGTTATTTCAAGTGATTATTATGGTAAAGAATAGTTTCGACCATTAAAAATATGTGAAGCTAGACGTACATCAAATACGTCAAGGCATATCCAAATCAAACGCACCTATGTAGGTGTGTTTTTTTAGTTTATCCAAt < 2:137461/141‑1 (MQ=255)gCCCTGTTATTTCAAGTGATTATTATGGTAAAGAATAGTTTCGACCATTAAAAATATGTGAAGCTAGACGTACATCAAATACGTCAAGGCATATCCAAATCAAACGCACCTATGTAGGTGTGTTTTTTTAGTTTATCCAAt < 2:227804/141‑1 (MQ=255) atGGTAAAGAATAGTTTCGACCATTAAAAATATGTGAAGCTAGACGTACATCAAATACGTCAAGGCATATCCAAATCAAACGCACCTATGTAGGTGTGTTTTTTTAGTTTATACAATGATTGATGTTATAATAATACTaaa > 2:128442/1‑141 (MQ=255) atGGTAAAGAATAGTTTCGACCATTAAAAATATGTGAAGCTAGACGTACATCAAATACGTCAAGGCATATCCAAATCAAACGCACCTATGTAGGTGTGTTTTTTTAGTTTATACAATGATTGATGTTATAATAATACTaaa > 2:73106/1‑141 (MQ=255) aGTTTCGACCATTAAAAATATGTGAAGCTAGACGTACATCAAATACGTCAAGGCATATCCAAATCAAACGCACCTATGTAGGTGTGTTTTTTTAGTTTATCCAATGATTGATGTTATAATAATACTAAATTTGTATCtata > 2:67668/1‑141 (MQ=255) aGTTTCGACCATTAAAAATATGTGAAGCTAGACGTACATCAAATACGTCAAGGCATATCCAAATCAAACGCACCTATGTAGGTGTGTTTTTTTAGTTTATCCAATGATTGATGTTATAATAATACTAAATTTGTATCtata > 2:21135/1‑141 (MQ=255) aGACGTACATCAAATACGTCAAGGCATATCCAAATCAAACGCACCTATGTAGGTGTGTTTTTTTAGTTTATCCAATGATTGATGTTATAATAATACTAAATTTGTATCTATAAAAAAGTAATGAGCATTTGTGCGCATatg < 2:194077/141‑1 (MQ=255) aCGTACATCAAATACGTCAAGGCATATCCAAATCAAACGCACCTATGTAGGTGTGTTTTTTTAGTTTATCCAATGATTGATGTTATAATAATACTAAATTTGTATCTATAAAAAAGTAATGAGCATTTGTGCGCATatgat > 1:169242/1‑141 (MQ=255) aCGTCAAGGCATATCCAAATCAAACGCACCTATGTAGGTGTGTTTTTTTTGTTTATTTAATGATTGATGTTATAATAATACTAAATTTGTATCTATAAAAAAGTAATGAGCATTTGTGCGCATATGATGATGTAAAGCGTa > 2:127125/1‑141 (MQ=255) aCGTCAAGGCATATCCAAATCAAACGCACCTATGTAGGTGTGTTTTTTTAGTTTATCCAATGATTGATGTTATAATAATACTAAATTTGTATCTATAAAAAAGTAATGAGCATTTGTGCGCATATGATGATGTAAAGCGTa > 2:157961/1‑141 (MQ=255) aGGCATATCCAAATCAAACGCACCTATGTAGGTGTGTTTTTTTAGTTTATCCAATGATTGATGTTATAATAATACTAAATTTGTATCTATAAAAAAGTAATGAGCATTTGTGCGCATATGATGATGTAAAGCGTAAATGTc > 1:179834/1‑141 (MQ=255) ggCATATCCAAATCAAACGCACCTATGTAGGTGTGTTTTTTTAGTTTATCCAATGATTGATGTTATAATAATACTAAATTTGTATCTATAAAAAAGTAATGAGCATTTGTGCGCATATGATGATGTAAAGCGTAAATGTCa > 1:262263/1‑141 (MQ=255) ggCATATCCAAATCAAACGCACCTATGTAGGCGTGTTTTTTTAGTTTATCCAATGATTGATGTTATAATAATACTAAATTTGTATCTATAAAAAAGTAATGAGCATTTGTGCGCATATGATGATGTAAAGCGTAAATGTCa > 2:105766/1‑141 (MQ=255) ccAAATCAAACGCACCTATGTAGGTGTGTTTTTTTTTTTTTTTTTATCATTGATGTTATAATAATACTATATTTGTATTTATAAAAAATTAGTAAGCCTTTGTGCGCATTTGATGCTGTAACGCGTAGATGACATAGCGat > 2:162031/1‑141 (MQ=255) tCAAACGCACCTATGTAGGTGTGTTTTTTTAGTTTATCCAATGATTGATGTTATAATAATACTAAATTTGTATCTATAAAAAAGTAATGAGCATTTGTGCGCATATGATGATGTAAAGCGTAAATGTCATAGTGATATTTa < 2:126615/141‑1 (MQ=255) aaCGCACCTATGTAGGTGTGTTTTTTTAGTTTATCCAATGATTGATGTTATAATAATACTAAATTTGTATCTATAAAAAAGTAATGAGCATTTGTGCGCATATGATGATGTAAAGCGTAAATGTCATAGTGATATTTAAgg < 1:218186/141‑1 (MQ=255) aaCGCACCTATGTAGGTGTGTTTTTTTAGTTTATCCAATGATTGATGTTATAATAATACTAAATTTGTATCTATAAAAAAGTAATGAGCATTTGTGCGCATATGATGATGTAAAGCGTAAATGTCATAGTGATATTTAAgg < 1:137525/141‑1 (MQ=255) aaCGCACCTATGTAGGTGTGTTTTTTTAGTTTATACAATGATTGATGTTATAATAATACTAAATTTGTATCTATAAAAAAGTAATGAGCATTTGTGCGCATATGATGATGTAAAGCGTAAATGTCATAGTGATATTTAAgg < 1:73106/141‑1 (MQ=255) aaCGCACCTATGTAGGTGTGTTTTTTTAGTTTATACAATGATTGATGTTATAATAATACTAAATTTGTATCTATAAAAAAGTAATGAGCATTTGTGCGCATATGATGATGTAAAGCGTAAATGTCATAGTGATATTTAAgg < 1:128442/141‑1 (MQ=255) cGCACCTATGTAGGTGTGTTTTTTTAGTTTATCCAATGATTGATGTTATAATAATACTAAATTTGTATCTATAAAAAAGTAATGAGCATTTGTGCGCATATGATGATGTAAAGCGTAAATGTCATAGTGATATTTAAGGga > 1:99964/1‑141 (MQ=255) tgtTTTTTTAGTTTATCCAATGATTGATGTTATAATAATACTAAATTTGTATCTATAAAAAAGTAATGAGCATTTGTGCGCATATGATGATGTAAAGCGTAAATGTCATAGTGATATTTAAGGGAGATTGAATATgaagaa > 1:199859/1‑141 (MQ=255) tgtTTTTTTAGTTTATCCAATGATTGATGTTATAATAATACTAAATTTGTATCTATAAAAAAGTAATGAGCATTTGTGCGCATATGATGATGTAAAGCGTAAATGTCATAGTGATATTTAAGGGAGATTGAATATgaagaa > 1:12534/1‑141 (MQ=255) | GCCCTGTTATTTCAAGTGATTATTATGGTAAAGAATAGTTTCGACCATTAAAAATATGTGAAGCTAGACGTACATCAAATACGTCAAGGCATATCCAAATCAAACGCACCTATGTAGGTGTGTTTTTTTAGTTTATCCAATGATTGATGTTATAATAATACTAAATTTGTATCTATAAAAAAGTAATGAGCATTTGTGCGCATATGATGATGTAAAGCGTAAATGTCATAGTGATATTTAAGGGAGATTGAATATGAAGAA > CP000730/1221548‑1221808 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |