Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	2,146,374	T→C	24.0%	L183S (TTA→TCA)	orf5 →	amidohydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,146,374	0	T	C	24.0%	39.0 / 11.7	25	L183S (TTA→TCA)	orf5	amidohydrolase
Reads supporting (aligned to +/- strand):  ref base T (14/5);  new base C (3/3);  total (17/8)
Fisher's exact test for biased strand distribution p-value = 3.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01

GGTACTTATGTGACCCAACTTATCTGTTATGATTTACGTTTTCCAGAATTATTAAGATATCCTGCTCGTAGTGGTGCTAAAATTGCATTTTATGTGGCGCAATGGCCTATGTCACGTCTTCAACATTGGCATTCATTACTAAAGGCACGTGCAATTGAAAATAATATGTTTGTCATTGGAACTAATAGCACTGGATTCGATGGTAACACAGAATATGCTGGTCATTCGATAGTTATAAACCCAAATGGAGATTTAGTTGGAGAATTAAATGAATC  >  CP000730/2146238‑2146512                                                                                                                                         |                                                                                                                                           ggTACTTATGTGACCCAACTTATCTGTTATGATTTACGTTTTCCAGAATTATTAAGATATCCTGCTCGTAGTGGTGCTAAAATTGCATTTTATGTGGCGCAATGGCCTATGTCACGTCTTCAACATTGGCATTCATTACTa                                                                                                                                        >  1:125326/1‑141 (MQ=255)   tACTTATGTGACCCAACTTATCTGTTATGATTTACGTTTTCCAGAATTATTAAGATATCCTGCTCGTAGTGGTGCTAAAATTGCATTTTATGTGGCGCAATGGCCTATGTCACGTCTTCAACATTGGCATTCATTACTaaa                                                                                                                                      <  2:152585/141‑1 (MQ=255)               ccAACTTATCTGTTATGATTTACGTTTTCCAGAATTATTAAGATATCCTGCTCGTAGTGGTGCTAAAATTGCATTTTATGTGGCGCAATGGCCTATGTCACGTCTTCAACATTGGCATTCATTACTAAAGGCACGTGCAAt                                                                                                                          >  1:229312/1‑141 (MQ=255)                   cTTATCTGTTATGTTTTACGTTTTCCAGAATTATTAAGATATCCTGCTCGTAGTGGTGCTAAAATTGCATTTTATGTGGCGCAATGGCCTATGTCACGTCTTCAACATTGGCATTCATTACTAAAGGCACGTGCAATTGaa                                                                                                                      <  2:249781/141‑1 (MQ=255)                        cTGTTATGATTTACGTTTTCCAGAATTATTAAGATATCCTGCTCGTAGTGGTGCTAAAATTGCATTTTATGTGGCGCAATGGCCTATGTCACGTCTTCAACATTGGCATTCATTACTAAAGGCACGTGCAATTGAAaataa                                                                                                                 >  2:16924/1‑141 (MQ=255)                        cTGTTATGATTTACGTTTTCCAGAATTATTAAGATATCCTGCTCGTAGTGGTGCTAAAATTGCATTTTATGTGGCGCAATGGCCTATGTCACGTCTTCAACATTGGCATTCATTACTAAAGGCACGTGCAATTGAAaataa                                                                                                                 >  2:106391/1‑141 (MQ=255)                        cTGTTATGATTTACGTTTTCCAGAATTATTAAGATATCCTGCTCGTAGTGGTGCTAAAATTGCATTTTATGTGGCGCAATGGCCTATGTCACGTCTTCAACATTGGCATTCATTACTAAAGGCACGTGCAATTGAAaataa                                                                                                                 >  2:189298/1‑141 (MQ=255)                        cTGTTATGATTTACGTTTTCCAGAATTATTAAGATATCCTGCTCGTAGTGGTGCTAAAATTGCATTTTATGTGGCGCAATGGCCTATGTCACGTCTTCAACATTGGCATTCATTACTAAAGGCACGTGCAATTGAAaataa                                                                                                                 >  2:33009/1‑141 (MQ=255)                        cTGTTATGATTTACGTTTTCCAGAATTATTAAGATATCCTGCTCGTAGTGGTGCTAAAATTGCATTTTATGTGGCGCAATGGCCTATGTCACGTCTTCAACATTGGCATTCATCACTAAAGGCACGTGCAATTGAAaataa                                                                                                                 >  2:206974/1‑141 (MQ=255)                        cTGTTATGATTTACGTTTTCCAGAATTATTAAGATATCCTGCTCGTAGTGGTGCTAAAATTGCATTTTATGTGGCGCAATGGCCTATGTCACGTCTTCAACATTGGCATTCATCACTAAAGGCACGTGCAATTGAAaataa                                                                                                                 >  2:176309/1‑141 (MQ=255)                        cTGTTATGATTTACGTTTTCCAGAATTATTAAGATATCCTGCTCGTAGTGGTGCTAAAATTGCATTTTATGTGGCGCAATGGCCTATGTCACGTCTTCAACATTGGCATTCATCACTAAAGGCACGTGCAATTGAAaataa                                                                                                                 >  1:230595/1‑141 (MQ=255)                         tGTTATGATTTACGTTTTCCAGAATTATTAAGATATCCTGCTCGTAGTGGTGCTAAAATTGCATTTTATGTGGCGCAATGGCCTATGTCACGTCTTCAACATTGGCATTCATTACTAAAGGCACGTGCAATTGAAaataat                                                                                                                >  2:164096/1‑141 (MQ=255)                              tGATTTACGTTTTCCAGAATTATTAAGATATCCTGCTCGTAGTGGTGCTAAAATTGCATTTTATGTGGCGCAATGGCCTATGTCACGTCTTCAACATTGGCATTCATTACTAAAGGCACGTGCAATTGAAAATAATATGtt                                                                                                           >  1:261105/1‑141 (MQ=255)                                    aCGTTTTCCAGAATTATTAAGATATCCTGCTCGTAGTGGTGCTAAAATTGCATTTTATGTGGCGCAATGGCCTATGTCACGTCTTCAACATTGGCATTCATTACTAAAGGCACGTGCAATTGAAAATAATATGTTTGTCAt                                                                                                     >  2:2217/1‑141 (MQ=255)                                          tCCAGAATTATTAAGATATCCTGCTCGTAGTGGTGCTAAAATTGCATTTTATGTGGCGCAATGGCCTATGTCACGTCTTCAACATTGGCATTCATTACTAAAGGCACGTGCAATTGAAAATAATATGTTTGTCATTGGAAc                                                                                               >  1:55110/1‑141 (MQ=255)                                                attattAAGATATCCTGCTCGTAGTGGTGCTAAAATTGCATTTTATGTGGCGCAATGGCCTATGTCACGTCTTCAACATTGGCATTCATTACTAAAGGCACGTGCAATTGAAAATAATATGTTTGTCATTGGAACTAATAg                                                                                         >  1:137865/1‑141 (MQ=255)                                                                    gTAGTGGTGCTAAAATTGCATTTTATGTGGCGCAATGGCCTATGTCACGTCTTCAACATTGGCATTCATTACTAAAGGCACGTGCAATTGAAAATAATATGTTTGTCATTGGAACTAATAGCACTGGATTCGATGGTAaca                                                                     >  2:179737/1‑141 (MQ=255)                                                                     tAGTGGTGCTAAAATTGCATTTTATGTGGCGCAATGGCCTATGTCACGTCTTCAACATTGGCATTCATTACTAAAGGCACGTGCAATTGAAAATAATATGTTTGTCATTGGAACTAATAGCACTGGATTCGATGGTAacac                                                                    <  2:143042/141‑1 (MQ=255)                                                                     tAGTGGTGCTAAAATTGCATTTTATGTGGCGCAATGGCCTATGTCACGTCTTCAACATTGGCATTCATTACTAAAGGCACGTGCAATTGAAAATAATATGTTTGTCATTGGAACTAATAGCACTGGATTCGATGGTAacac                                                                    <  2:75633/141‑1 (MQ=255)                                                                                       aTTTTATGTGGCGCAATGGCCTATGTCACGTCTTCTACATTGCCATTCATTACTAAAGGCACGTGCATTTGAAAATAATATGTTTGTCATTGGAACTAATAGCACTGGATTCGATGGTAACACAGAATATGCTGGTCATTc                                                  >  1:227906/1‑141 (MQ=255)                                                                                       aTTTTATGTGGCGCAATGGCCTATGTCACGTCTTCAACATTGGCATTCATTACTAAAGGCACGTGCAATTGAAAATAATATGTTTGTCATTGGAACTAATAGCACTGGATTCGATGGTAACACAGAATATGCTGGTCATTc                                                  >  1:132124/1‑141 (MQ=255)                                                                                                                     tCTTCAACATTGGCATTCATCACTAAAGGCACGTGCAATTGAAAATAATATGTTTGTCATTGGAACTAATAGCACTGGATTCGATGGTAACACAGAATATGCTGGTCATTCGATAGTTATAAACCCAAATGGAGATTTAGt                    <  1:206974/141‑1 (MQ=255)                                                                                                                     tCTTCAACATTGGCATTCATCACTAAAGGCACGTGCAATTGAAAATAATATGTTTGTCATTGGAACTAATAGCACTGGATTCGATGGTAACACAGAATATGCTGGTCATTCGATAGTTATAAACCCAAATGGAGATTTAGt                    <  2:230595/141‑1 (MQ=255)                                                                                                                     tCTTCAACATTGGCATTCATCACTAAAGGCACGTGCAATTGAAAATAATATGTTTGTCATTGGAACTAATAGCACTGGATTCGATGGTAACACAGAATATGCTGGTCATTCGATAGTTATAAACCCAAATGGAGATTTAGt                    <  1:176309/141‑1 (MQ=255)                                                                                                                              ttGGCATTCATTACTAAAGGCACGTGCAATTGAAAATAATATGTTTGTCATTGGAACTAATAGCACTGGATTCGATGGTAACACAGAATATGCTGGTCATTCGATAGTTATAAACCCAAATGGAGATTTaattctccaact           <  2:205820/141‑12 (MQ=255)                                                                                                                                    ttcattACTAAAGGCACGTGCAATTGAAAATAATATGTTTGTCATTGGAACTAATAGCACTGGATTCGATGGTAACACAGAATATGCTGGTCATTCGATAGTTATAAACCCAAATGGAGATTTAGTTGGAGAATTAAATGa     <  2:137865/141‑1 (MQ=255)                                                                                                                                       attACTAAAGGCACGTGCAATTGAAAATAATATGTTTGTCATTGGAACTAATAGCACTGGATTCGATGGTAACACAGAATATGCTGGTCATTCGATAGTTATAAACCCAAATGGAGATTTAGTTGGAGAATTAAATGAATc  <  1:22459/141‑1 (MQ=255)                                                                                                                                         |                                                                                                                                           GGTACTTATGTGACCCAACTTATCTGTTATGATTTACGTTTTCCAGAATTATTAAGATATCCTGCTCGTAGTGGTGCTAAAATTGCATTTTATGTGGCGCAATGGCCTATGTCACGTCTTCAACATTGGCATTCATTACTAAAGGCACGTGCAATTGAAAATAATATGTTTGTCATTGGAACTAATAGCACTGGATTCGATGGTAACACAGAATATGCTGGTCATTCGATAGTTATAAACCCAAATGGAGATTTAGTTGGAGAATTAAATGAATC  >  CP000730/2146238‑2146512

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑