Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913_3_bme_pgi 257,908 Δ776 bp [crl] [crl]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913_3_bme_pgi 257908–258676 258683 8–776 213 [0] [0] 218 [crl] [crl]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_bme_pgi = 2579070 (0.000)202 (1.150) 105/180 0.0 100% intergenic (+8/‑769) crl/crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers/pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers
?NC_000913_3_bme_pgi 258684 = 0 (0.000)pseudogene (9/331 nt) crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers

Only 100 of 218 total aligned reads displayed.

GAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTGAAGGTAAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCT  >  NC_000913_3_bme_pgi/258586‑258783
                                                                                                  |                                                                                                   
atcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAg                                                                                                    >  2:3634405‑M2/99‑100 (MQ=255)
atcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAg                                                                                                    <  1:1530403‑M2/2‑1 (MQ=255)
 tcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAg                                                                                                    <  2:1306412‑M2/2‑1 (MQ=255)
 tcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGt                                                                                                   >  1:2039663‑M2/98‑100 (MQ=255)
 tcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGt                                                                                                   >  2:3500851‑M2/98‑100 (MQ=255)
 tcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcctgaaggtaAGt                                                                                                   >  2:3031535‑M2/98‑100 (MQ=255)
  cacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTg                                                                                                  >  1:896657‑M2/97‑100 (MQ=255)
    caacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGc                                                                                                 >  2:3583276‑M2/95‑99 (MQ=255)
    caacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGc                                                                                                 >  2:198837‑M2/95‑99 (MQ=255)
    caacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGc                                                                                                 >  1:4406814‑M2/95‑99 (MQ=255)
    caacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGc                                                                                                 >  2:4732530‑M2/95‑99 (MQ=255)
      acaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCaaa                                                                                              <  1:1289452‑M2/8‑1 (MQ=255)
       caggagatagcaatgacgttaacgagtggacacccgaagagcagattgatgaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAg                                                                                             <  2:2172142‑M2/9‑1 (MQ=255)
         ggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGa                                                                                            >  1:881705‑M2/90‑99 (MQ=255)
         ggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGa                                                                                            >  1:3465515‑M2/90‑99 (MQ=255)
            gatagcgatgacgtgaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAAt                                                                                        <  2:3657834‑M2/14‑1 (MQ=255)
                 caatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGAtt                                                                                   >  1:2820048‑M2/82‑100 (MQ=255)
                  aatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTc                                                                                  >  2:3440171‑M2/81‑100 (MQ=255)
                  aatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggaccgtatattcgtgaaggtaAGTGCAAAGATAATAGatta                                                                                  >  2:1529935‑M2/81‑99 (MQ=255)
                  aatgacgttaccgagtggacacccgaagagcaaaaaaaaaaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGa                                                                                     <  2:4655328‑M2/17‑1 (MQ=255)
                   atgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCt                                                                                 <  2:326232‑M2/21‑1 (MQ=255)
                    tgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCtt                                                                                <  1:1164150‑M2/22‑1 (MQ=255)
                      acgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCttt                                                                               <  1:3009576‑M2/23‑1 (MQ=255)
                        gttaccgagtggacaccctaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTc                                                                            <  1:3651376‑M2/26‑1 (MQ=255)
                        gttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTc                                                                            <  2:89473‑M2/26‑1 (MQ=255)
                         ttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCg                                                                           <  2:845586‑M2/27‑1 (MQ=255)
                         ttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCg                                                                           <  2:1882696‑M2/27‑1 (MQ=255)
                           accgagtggacacccgaagagcagattgatcaaaaaatgtaccgcactaggcccgtatactcgtgaaggtaAGTGCAACGATAATCGATACTTTTtcgct                                                                         >  2:4711050‑M2/72‑98 (MQ=255)
                            ccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGAt                                                                         <  1:1666800‑M2/29‑1 (MQ=255)
                             cgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTg                                                                       >  2:681032‑M2/70‑100 (MQ=255)
                             cgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTg                                                                       >  1:1750744‑M2/70‑100 (MQ=255)
                             cgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTg                                                                       >  1:4169774‑M2/70‑100 (MQ=255)
                              gagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGt                                                                      >  2:1210997‑M2/69‑100 (MQ=255)
                               agtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTc                                                                     <  2:472592‑M2/33‑1 (MQ=255)
                               agtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTc                                                                     <  1:4028994‑M2/33‑1 (MQ=255)
                                  ggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTgg                                                                  >  1:2557943‑M2/65‑100 (MQ=255)
                                  ggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTgg                                                                  >  2:3200652‑M2/65‑100 (MQ=255)
                                  ggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTgg                                                                  >  2:1278519‑M2/65‑100 (MQ=255)
                                   gacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGc                                                                 <  1:2998883‑M2/37‑1 (MQ=255)
                                       cccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTa                                                             >  1:650039‑M2/60‑100 (MQ=255)
                                       cccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTa                                                             >  1:2308436‑M2/60‑100 (MQ=255)
                                       cccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTa                                                             >  1:1846948‑M2/60‑100 (MQ=255)
                                       cccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTa                                                             <  1:1257666‑M2/41‑1 (MQ=255)
                                       cccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTa                                                             >  1:2916441‑M2/60‑100 (MQ=255)
                                        ccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTAt                                                            <  1:3924122‑M2/42‑1 (MQ=255)
                                         cgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATg                                                           >  1:1033118‑M2/58‑100 (MQ=255)
                                         cgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCCTTTTCGATTGTCTGGCTGTATg                                                           <  2:2282436‑M2/43‑1 (MQ=255)
                                            agagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGt                                                        <  2:4478710‑M2/46‑1 (MQ=255)
                                             gagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTc                                                       <  1:2765913‑M2/47‑1 (MQ=255)
                                             gagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTc                                                       <  2:3152804‑M2/47‑1 (MQ=255)
                                              agcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTc                                                       >  2:3791664‑M2/53‑99 (MQ=255)
                                               gcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCaa                                                     >  1:2153730‑M2/52‑100 (MQ=255)
                                               gcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCaa                                                     >  2:2685115‑M2/52‑100 (MQ=255)
                                                cagaatgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTa                                                             <  2:2359089‑M2/41‑1 (MQ=255)
                                                 agattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACg                                                   <  2:4160386‑M2/51‑1 (MQ=255)
                                                  gattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGt                                                  >  1:2669875‑M2/49‑100 (MQ=255)
                                                  gattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGt                                                  >  2:3285392‑M2/49‑100 (MQ=255)
                                                    ttgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTg                                                 <  1:3166534‑M2/53‑1 (MQ=255)
                                                      gatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGaaa                                              >  1:3333114‑M2/45‑100 (MQ=255)
                                                       atcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAAc                                             >  2:4005538‑M2/44‑100 (MQ=255)
                                                       atcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAAc                                             >  1:1464438‑M2/44‑100 (MQ=255)
                                                         caaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCg                                           >  2:4065458‑M2/42‑100 (MQ=255)
                                                         caaaaaatttaccgcactaggccagtatattcgtgaaggtgAGTGCAAAGATAATCGACTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTCAAACCg                                           >  1:3777131‑M2/42‑100 (MQ=255)
                                                          aaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCgg                                          >  1:575322‑M2/41‑100 (MQ=255)
                                                          aaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCg                                           >  2:4313614‑M2/41‑99 (MQ=255)
                                                           aaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGc                                         <  2:1595949‑M2/61‑1 (MQ=255)
                                                            aaaatttaccgcactaggcccgtatattcgtgaagctaAGTGCAAAGATAATCGACTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCa                                        >  2:3378587‑M2/39‑100 (MQ=255)
                                                              aatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCAcc                                      <  1:3912155‑M2/64‑1 (MQ=255)
                                                               atttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCg                                     <  2:1404759‑M2/65‑1 (MQ=255)
                                                                    ccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGt                                >  2:2508545‑M2/31‑100 (MQ=255)
                                                                    ccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGt                                >  1:1564596‑M2/31‑100 (MQ=255)
                                                                      gcactaggctcgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGAATGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGc                              <  1:4723806‑M2/72‑1 (MQ=255)
                                                                      gcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGt                                <  2:4623098‑M2/70‑1 (MQ=255)
                                                                       cactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCg                             >  2:244611‑M2/28‑100 (MQ=255)
                                                                       cactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCg                             <  1:4747114‑M2/73‑1 (MQ=255)
                                                                       cactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCg                             <  1:4242576‑M2/73‑1 (MQ=255)
                                                                         ctaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTg                           <  1:4292374‑M2/75‑1 (MQ=255)
                                                                            ggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGaa                         <  1:469785‑M2/77‑1 (MQ=255)
                                                                            ggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAAt                        >  2:2098178‑M2/23‑100 (MQ=255)
                                                                            ggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAAt                        <  1:1907027‑M2/78‑1 (MQ=255)
                                                                             gcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAAtt                       <  1:3090930‑M2/79‑1 (MQ=255)
                                                                                  tatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTggg                  <  1:3460770‑M2/84‑1 (MQ=255)
                                                                                  tatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTggg                  <  2:374635‑M2/84‑1 (MQ=255)
                                                                                    tattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTgggg                 <  2:3460119‑M2/85‑1 (MQ=255)
                                                                                    tattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGc                <  2:883853‑M2/86‑1 (MQ=255)
                                                                                      ttcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCt               <  1:3041934‑M2/87‑1 (MQ=255)
                                                                                      ntcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCtg              >  1:2800652‑M2/13‑100 (MQ=255)
                                                                                         gtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCtggtg           <  2:3343483‑M2/91‑1 (MQ=255)
                                                                                            aaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGtggat        <  1:1264019‑M2/94‑1 (MQ=255)
                                                                                             aggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGtggatg       <  2:2556281‑M2/95‑1 (MQ=255)
                                                                                              ggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGtggatgg      <  1:1342611‑M2/96‑1 (MQ=255)
                                                                                              ggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGtggatgg      <  1:239729‑M2/96‑1 (MQ=255)
                                                                                                taAGTGCAAAGATAGTCGATTCTTTTTCGATTGTCTGGCTGTGGGCGTCAGCGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAg    <  2:3840097‑M2/98‑1 (MQ=255)
                                                                                                taAGTGCAAAGATAATCGATTCTTTTTTTTTTTTTTTGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAg    >  2:501331‑M2/3‑100 (MQ=255)
                                                                                                 aAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCtggtg           >  2:1072899‑M2/2‑92 (MQ=255)
                                                                                                  aGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGc   <  1:1715096/99‑1 (MQ=255)
                                                                                                  aGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGc   <  2:3085203/99‑1 (MQ=255)
                                                                                                  aGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGc   <  2:741496/99‑1 (MQ=255)
                                                                                                  aGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGc   <  2:1965876/99‑1 (MQ=255)
                                                                                                  aGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCt  <  1:1991821/100‑1 (MQ=255)
                                                                                                  |                                                                                                   
GAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTGAAGGTAAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCT  >  NC_000913_3_bme_pgi/258586‑258783

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: