| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913_3_bme_pgi | 4,561,548 | C→T | A377T (GCG→ACG) | yjiJ ← | DUF1228 family putative inner membrane MFS superfamily transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913_3_bme_pgi | 4,561,548 | 0 | C | T | 98.0% | 163.8 / NA | 51 | A377T (GCG→ACG) | yjiJ | DUF1228 family putative inner membrane MFS superfamily transporter |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (30/20); minor base A (1/0); total (31/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCGAAACGAGGCCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATGAGCGTCACAAAGCCTAAAAGATTGAGATTTCCCG > NC_000913_3_bme_pgi/4561402‑4561694 | gCGAAACGAGGCCTCCCTTTCTGGCAATCGGAAAACTGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCgtagc > 2:829311/1‑150 (MQ=255)gCGAAACGAGGCCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCgtagc < 1:754073/150‑1 (MQ=255) aaCGAGGCCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCCCTTGTAGCTTGAATAATTGCACTGTGCTGATAAGCGTAGCAATa > 1:570049/1‑150 (MQ=255) aaGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCgtag < 2:513769/124‑1 (MQ=255) aaGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCgtag > 1:513769/1‑124 (MQ=255) aaGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGTAGCAATAAACAACGCCGCCGCGCCGCag > 2:1126972/1‑150 (MQ=255) tGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGTAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAg < 1:825470/150‑1 (MQ=255) tGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGTAGCAATAAACAACGCCGCCGCACCGCAGAGAGTGGCGCTGGCAAg > 1:491474/1‑150 (MQ=255) accataTCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGTAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAgcgc > 2:874133/6‑150 (MQ=255) aCATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTATGGGAAACTTTCCTCATTAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGTAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGa < 2:1336315/150‑1 (MQ=255) aCATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGTAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGa < 2:1227013/150‑1 (MQ=255) aCATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGTAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGa > 2:469275/1‑150 (MQ=255) aTCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGTAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATg > 1:696695/1‑150 (MQ=255) aaCGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGTAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTc > 1:321317/1‑150 (MQ=255) aaCGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGTAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTc < 1:1341324/150‑1 (MQ=255) aaCGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGTAGCAATAAACAACGCCGCCGCGCCGACGAGAGTGCCGCTGACTACCGCCGATGGTc > 2:577396/1‑150 (MQ=255) aaCGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGTAGCAATAAACAACACCACCGCGCCGCAGAGAGTGACGCTGGCAAGCCCAGATCTTc > 2:1239520/1‑150 (MQ=255) aaCGGTTGCGGTTGAGGTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGTAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTc < 2:86115/150‑1 (MQ=255) gttgcggttgAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGTAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCAtt > 1:461811/1‑150 (MQ=255) ggttgAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGTAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGa < 1:1167243/150‑1 (MQ=255) ggttgAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGTAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGa < 1:785698/150‑1 (MQ=255) ttgAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGTAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATGGCTGAgg > 2:917122/1‑149 (MQ=255) tgAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGTAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCa < 1:1126972/150‑1 (MQ=255) ttttCTTGAGGGGTAAGGGAAACTTCTCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGTAGCAATAAACAACGCCGTCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATAc < 1:1350011/149‑1 (MQ=255) cTTGAGTGGTAAGGGAAACTTCCCTGATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATGAGCGTAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGACGCTGGCAAGCGCCGATGTTGCATTGCTGAGCATACTGGt > 1:1061662/1‑150 (MQ=255) ttGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGTAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCACGCGCCGATGTTCCATTCCTCAGCATACTGGta > 2:50149/1‑149 (MQ=255) ttGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGTAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTc > 2:1360954/1‑150 (MQ=255) ttGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGTAGCAATAAACAACGCCGCCGCGCCGCAGAGAGGGGCGCTGGCAAGCGACGCGGTTGCAGTGCTGGGCATACTGGTc > 1:471857/1‑150 (MQ=255) tAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGTAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCgg < 2:102846/150‑1 (MQ=255) gggAAACTTCCCTCATGAAGTGACCACTTGTAGCTTGAATGATTTCACGGTGCTGATAAGCGTAGCAATAAACAACGCAGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGGTCCATTGCTGAGCATACTGGCCAGCGCCGGGcg > 1:334060/1‑149 (MQ=255) ggAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAACTACTAGCACTGTGATGATACGCGAAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCg > 2:1341386/1‑150 (MQ=255) gacaCTTCCCCCAGGAAGTGACCACGTGTAGCTTAAATAATTGCACGGTGCCGATAAGCGTAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGCCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGa < 1:1278828/147‑1 (MQ=255) aaaCTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTCCTGATAAGCGTAGCAATAACCACCGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTACCCTTACTGGCCACCGCCGGGCCgcg > 2:485652/1‑148 (MQ=255) cTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGTAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGAt < 1:469275/150‑1 (MQ=255) tGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGTAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGCGGATTTGCCCAAt > 2:801693/1‑150 (MQ=255) aaGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGTAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCCCCGGGCCGAGGCTTTGacccctgc > 2:969724/1‑145 (MQ=255) ccACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGTAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTaaa > 2:74834/1‑150 (MQ=255) cACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGTAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGACGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGATCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAAt > 1:582246/1‑150 (MQ=255) cTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGTAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATGa > 2:21725/1‑150 (MQ=255) cTTGTAGCTTAAATAATTGCACTGGGCTGATAAGCGTAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATGa < 1:440950/150‑1 (MQ=255) ttGTAGCTTAAATAATTGCACTGTGCTGATAAGCGTAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGATGAGCATACAGGTCAGAGCCAGGACGAGGCTTTGCCCCATGCCGTCAATgca > 1:991010/1‑148 (MQ=255) tGTAGCTTAAATAATTGCACTGTGCTGATAAGCGTAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATGAGc < 1:700473/150‑1 (MQ=255) tAGCTTAAATAATTGCACTGTGCTGATAAGCGTAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATGAGCGt > 2:1017854/1‑150 (MQ=255) aGCTTAAATAATTGCACTGTGCTGATAAGCGTAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATGAGCGTc < 1:874133/150‑1 (MQ=255) cTTAAATAATTGCACTGTGCTGATAAGCGTAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCTCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATGAGGGTcac > 1:1175006/1‑150 (MQ=255) taatTGCACTGTGCTGATAAGCGTAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATGa < 1:872439/137‑1 (MQ=255) taatTGCACTGTGCTGATAAGCGTAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATGa > 2:872439/1‑137 (MQ=255) gCACTGTGCTGATAAGCGTAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATGAGCGTCACAAAGCCTaaaa < 1:829311/150‑1 (MQ=255) aCTGTGCTGATAAGCGTAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATGAGCGTCACAAAGCCTAAAAGa > 2:382478/1‑150 (MQ=255) aCTGTGCTGATAAGCGTAGCAATAAACAACGCCCCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCCGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATGAGCGTCACCAAGCCTAAAAgc > 2:257329/1‑149 (MQ=255) tgtgCTGATAAGCGTAGCAATAAACAACGCCGCCGCGCCGCAGGGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATGAGCGTCACAAAGCCTAAAAGAtt < 1:679813/150‑1 (MQ=255) gtgCTGATAAGCGTAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATGAGCGTCACAAAGCCTAAAAGATTg < 2:1221759/150‑1 (MQ=255) tAAGCGTAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATGAGCGTCACAAAGCCTAAAAGATTGAGATTTc > 1:1364882/1‑150 (MQ=255) aaGCGTAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATGAGCGTCACAAAGCCTAAAAGATTGAGCTTTcc > 1:900894/1‑150 (MQ=255) aGCGTAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATCCTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATGAGCGTCACAAAGCCTAAAAGATTGAGATTTccc < 1:577396/150‑1 (MQ=255) gcgtAGCAATAAACAACGCCGCCGCGCCGCAGGGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATGAGCGTCACAAAGCCTAAAAGATTGAGATTTCCCg < 1:74834/150‑1 (MQ=255) gcgtAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATGAGCGTCACAAAGCCTAAAAGATTGAGATTTCCCg > 1:1192929/1‑150 (MQ=255) | GCGAAACGAGGCCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATGAGCGTCACAAAGCCTAAAAGATTGAGATTTCCCG > NC_000913_3_bme_pgi/4561402‑4561694 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |