BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_KO_pgi	4,294,815	+GC	intergenic (+587/+55)	gltP → / ← yjcO	glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_KO_pgi	4,294,814	1	.	C	94.6%	524.5 / 6.0	168	intergenic (+586/+56)	gltP/yjcO	glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
Reads supporting (aligned to +/- strand): ref base . (0/1); major base C (82/76); minor base T (4/5); total (86/82)
Fisher's exact test for biased strand distribution p-value = 7.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	NC_000913_3_KO_pgi	4,294,814	2	.	G	99.4%	567.3 / ‑3.9	168	intergenic (+586/+56)	gltP/yjcO	glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
Reads supporting (aligned to +/- strand): ref base . (0/1); new base G (86/81); total (86/82)
Fisher's exact test for biased strand distribution p-value = 4.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 168 total aligned reads displayed.

GCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAG‑GCGTTTA‑‑CCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTT  >  NC_000913_3_KO_pgi/4294728‑4294954

||

gCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAG‑GCGTTTATGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAAcc                                                                                 >  2:2331355/1‑150 (MQ=1)

  gACGCTTAACGCGTCTTATCAGGCCCACGCCAGCCAGCGCAATACCCTCATTTAGCGTGACTTCGTCGTTTGCCC‑AAG‑GTGTTTACGCCGCAGCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGt                                                                               <  3:403750/150‑1 (MQ=11)

   aCGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAG‑GCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGtt                                                                              <  3:428476/150‑1 (MQ=255)

   aCGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAG‑GCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGtt                                                                              >  1:583382/1‑150 (MQ=255)

    cGCTTCACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAG‑GCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTg                                                                             <  1:33269/150‑1 (MQ=255)

    cGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAG‑GCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTg                                                                             <  2:2307869/150‑1 (MQ=255)

     gCTTAACGCGTCTTATCAGGCCTACTCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAG‑GCGTTTACGCCCCATCCTACATCAATGCCTGATGCGACGCTTGCCTCGTCTTATCAGGCCTATCTTAACCGTTgg                                                                            >  1:654063/1‑150 (MQ=11)

     gCTTAACGCGTCTTATCAGGCCTACGCCAGACAGTTTAATAGCATGATTTAGCGTGCTTTTGTAGGTCGGAG‑AAG‑ACGTTTATGCCGCATCCGGCATCAATGCCTGTTGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTgg                                                                            <  4:1271211/150‑1 (MQ=11)

     gCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAG‑GCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTgg                                                                            >  2:2539938/1‑150 (MQ=255)

     gCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAG‑GCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTgg                                                                            >  1:844970/1‑150 (MQ=255)

     gCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAG‑GCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTgg                                                                            >  1:1498678/1‑150 (MQ=255)

       ttAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAG‑GCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGtt                                                                          >  4:1610428/1‑150 (MQ=255)

       ttAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAG‑GCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGtt                                                                          >  3:466896/1‑150 (MQ=255)

        tAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAG‑GCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTa                                                                         >  4:203157/1‑150 (MQ=255)

         aaCGCGTGTTATCAGGCCTACGGAAGACAGCGCAAAAGCCTAATTTCGCGTGATATTGTACGTTGGATCAAGCGGGTGCG‑‑CTTCCGCCGTCATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTaa                                                                        <  2:227137/150‑1 (MQ=2)

         aaCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAG‑GCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTaa                                                                        >  1:2062472/1‑150 (MQ=255)

         aaCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAG‑GCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTaa                                                                        >  1:1519564/1‑150 (MQ=255)

            gcgTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAG‑GCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAAttt                                                                     >  2:430210/1‑150 (MQ=255)

            gcgTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAG‑GCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAAttt                                                                     >  2:92536/1‑150 (MQ=255)

             cgTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAG‑GCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAAtttt                                                                    <  4:129533/150‑1 (MQ=255)

              gTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAG‑GCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAAttttt                                                                   <  4:1540229/150‑1 (MQ=255)

              gTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAG‑GCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTGGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAAttttt                                                                   >  2:411414/1‑150 (MQ=255)

               tCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAG‑GCGTTTATGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTCGGTTAAtccttc                                                                  >  2:1317049/1‑145 (MQ=11)

               tCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAG‑GCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTc                                                                  >  2:2057382/1‑150 (MQ=255)

                 ttATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAG‑GCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCaa                                                                >  1:2493843/1‑150 (MQ=255)

                 ttATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAG‑GCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCaa                                                                >  3:1587663/1‑150 (MQ=255)

                  tctaaGGCCTACGCCAGACAGAGCATCAGTCTGTTTTCGCGTGATTTCGTAGCTCGGAT‑AAG‑GCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCaaa                                                               <  2:2483555/146‑1 (MQ=14)

                  tATCAGGCCTACGTCGGAAAGCGAGATAGTTCCATTTAGCGTGATTTTGTAGGTCGGAT‑AAG‑GCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCaaa                                                               <  2:1584135/150‑1 (MQ=14)

                  tATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAG‑GCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCaaa                                                               <  1:2539938/150‑1 (MQ=255)

                   aTCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAG‑GCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAAc                                                              >  1:1956842/1‑150 (MQ=255)

                    tCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGAAGGTCGGAT‑AAG‑GCGTTTACGCCGCATCCGACATCAATGCCTGATGCGCCGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAAct                                                             >  2:1621150/1‑150 (MQ=14)

                      aGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAG‑GCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAActct                                                           >  4:703088/1‑150 (MQ=255)

                       ggCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAG‑GCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCtt                                                          <  2:2353755/150‑1 (MQ=255)

                       ggCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAG‑GCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCtt                                                          <  1:1317049/150‑1 (MQ=255)

                       ggCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAG‑GCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCtt                                                          <  1:1343724/150‑1 (MQ=255)

                       ggCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAG‑GCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCtt                                                          >  2:1213363/1‑150 (MQ=255)

                       ggCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAG‑GCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCtt                                                          >  4:134305/1‑150 (MQ=255)

                       ggCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAG‑GCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCtt                                                          <  3:1355731/150‑1 (MQ=255)

                        gCCTACGCGAGACAGCGGAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑ACG‑GCGTTTACGCCGCATCCGACATCAATGTCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTc                                                         <  4:1159018/150‑1 (MQ=17)

                        gCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAG‑GCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTc                                                         <  2:1660075/150‑1 (MQ=255)

                         ccTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAG‑GCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTca                                                        <  2:506008/150‑1 (MQ=255)

                         ccTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAG‑GCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAAACGTTGGTTAATTTTTCAAACTCTTca                                                        >  4:1333853/1‑150 (MQ=255)

                          ttACGCCGGACAGCGCAGTAGGCTGGTTTAGCGTGATTTTGTTGGTCGGAT‑AAG‑GCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTcac                                                       <  2:49785/149‑1 (MQ=17)

                           tACGCCAGACAGCGTAATACCCTGATTTCGCGTAATTTTGTAGGACGGAT‑AAG‑GCGTGTACGCCCCATCCGACATCAATGCCCGATGCGACGCTTTTCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTGCAAACTCCTcaca                                                      >  4:721829/1‑150 (MQ=21)

                           tACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAG‑GCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGt                                                                           <  4:1606775/129‑1 (MQ=255)

                           tACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAG‑GCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGt                                                                           >  3:1606775/1‑129 (MQ=255)

                           tACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAG‑GCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGt                                                                           >  3:1606789/1‑129 (MQ=255)

                           tACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAG‑GCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTcaca                                                      >  4:623340/1‑150 (MQ=255)

                            aCGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAG‑GCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCacac                                                     <  3:264138/150‑1 (MQ=255)

                            aCGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAG‑GCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCacac                                                     >  4:773388/1‑150 (MQ=255)

                              gCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAG‑GCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAccc                                                   <  2:2457587/150‑1 (MQ=255)

                              gCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAG‑GCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAccc                                                   <  2:1721136/150‑1 (MQ=255)

                                 cgACAGCGGACTCGGCTAATTTCGCGTGATTTTGTAGCTCGGAT‑ATG‑GCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGt                                                <  4:662685/149‑1 (MQ=17)

                                 aGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAG‑GCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGt                                                <  1:2037216/150‑1 (MQ=255)

                                    cAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAG‑GCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAGCCGTTGCTTAATCTTTCTATCTCTTCACACCCGGTATc                                             >  2:2043056/1‑150 (MQ=14)

                                    cAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAG‑GCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATc                                             >  2:2429118/1‑150 (MQ=255)

                                       cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAG‑GCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa                                          <  1:2043056/150‑1 (MQ=255)

                                       cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAG‑GCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa                                          >  3:1276209/1‑150 (MQ=255)

                                          aaTAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAG‑GCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAAccc                                       >  3:885720/1‑150 (MQ=255)

                                          aaTAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAG‑GCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAAccc                                       <  3:646647/150‑1 (MQ=255)

                                          aaTAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAG‑GCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAAccc                                       >  3:254124/1‑150 (MQ=255)

                                           aTAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAG‑GCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCt                                      >  3:505261/1‑150 (MQ=255)

                                            tAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAG‑GCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTT‑ATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTc                                    <  1:1055310/150‑1 (MQ=18)

                                            tAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAG‑GCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTT‑ATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTc                                    <  1:1055297/150‑1 (MQ=18)

                                            atgCCTGGTTTAGCGTGATTTTGTAGGTCGGAT‑AAA‑GCGTTTATGCCGTATACGACATCCATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCtt                                     <  2:1081605/148‑1 (MQ=21)

                                             aGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAG‑GCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTc                                    >  1:98963/1‑150 (MQ=255)

                                             aGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAG‑GCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTc                                    <  1:138741/150‑1 (MQ=255)

                                              gCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAG‑GCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTcc                                   <  1:2289560/150‑1 (MQ=255)

                                                cTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAG‑GCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCAt                                 <  1:1486977/150‑1 (MQ=255)

                                                 tGATTTAGCGTGATTTTGTAGGTCGGAT‑AAG‑GCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATa                                >  4:217985/1‑150 (MQ=255)

                                                   aTTTAGCGTGATTTTGTAGGTCGGAT‑AAG‑GCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACa                              <  2:1368115/150‑1 (MQ=255)

                                                    tttAGCGTGATTTTGTAGGTCGGAT‑AAG‑GCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAg                             <  3:1379733/150‑1 (MQ=255)

                                                       aGCGTGATTTTGTAGGTCGGAT‑AAG‑GCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTc                          <  2:583382/150‑1 (MQ=255)

                                                        gCGTGATTTTGTAGGTCGGAT‑AAG‑GCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCa                         <  1:411414/150‑1 (MQ=255)

                                                        gCGTGATTTTGTAGGTCGGAT‑AAG‑GCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCa                         >  1:847435/1‑150 (MQ=255)

                                                         cGTGATTTTGTAGGTCGGAT‑AAG‑GCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCtt                                     >  1:75778/1‑137 (MQ=255)

                                                         cGTGATTTTGTAGGTCGGAT‑AAG‑GCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCtt                                     <  2:75778/137‑1 (MQ=255)

                                                         cGTGATTTTGTAGGTCGGAT‑AAG‑GCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAg                        >  1:482519/1‑150 (MQ=255)

                                                            gATTTTGTAGGTCGGAT‑AAG‑GCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGtt                     <  3:119279/150‑1 (MQ=255)

                                                             aTTTTGTAGGTCGGAT‑AAG‑GCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTc                    <  1:1380896/150‑1 (MQ=255)

                                                             aTTTTGTAGGTCGGAT‑AAG‑GCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTc                    >  2:2051367/1‑150 (MQ=255)

                                                              ttttGTAGGTCGGAT‑AAG‑GCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCa                   >  3:140081/1‑150 (MQ=255)

                                                              ttttGTAGGTCGGAT‑AAG‑GCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCtt                                                          >  2:1332374/1‑111 (MQ=17)

                                                               tttGTAGGTCGGAT‑AAG‑GCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCaa                  <  2:1387027/150‑1 (MQ=255)

                                                               tttGTAGGTCGGAT‑AAG‑GCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCaa                  <  4:159915/150‑1 (MQ=255)

                                                                ttGTAGGTCGGAT‑AAG‑GCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAAc                 <  1:1907152/150‑1 (MQ=255)

                                                                ttGTAGGTCGGAT‑AAG‑GCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAAc                 <  3:1022388/150‑1 (MQ=255)

                                                                  gTAGGTCGGAT‑AAG‑GCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAAtttt                                                                    <  2:53348/97‑1 (MQ=17)

                                                                  gTAGGTCGGAT‑AAG‑GCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCa               <  1:2429118/150‑1 (MQ=255)

                                                                  gTAGGTCGGAT‑AAG‑GCGTTTACGCCGCATCAGACATCAATGCCTGATGCGACGCTTGCCGCGCCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAAACCTTGCATAGCGCTCAGGTTCAACCa               >  3:88665/1‑150 (MQ=21)

                                                                     ggTCGGAT‑AAG‑GCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTg            >  1:2185232/1‑150 (MQ=255)

                                                                      gTCGGAT‑AAG‑GCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATAc                               >  1:1698314/1‑130 (MQ=255)

                                                                      gTCGGAT‑AAG‑GCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGc           >  1:369066/1‑150 (MQ=255)

                                                                       tCGGAT‑AAG‑GCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCa          <  4:254124/150‑1 (MQ=255)

                                                                       tCGGAT‑AAG‑GCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCa          >  3:221617/1‑150 (MQ=255)

                                                                       tCGGAT‑AAG‑GCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCa          <  4:1091049/150‑1 (MQ=255)

                                                                         ggAT‑AAG‑GCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAgc        >  1:79100/1‑150 (MQ=255)

                                                                          gAT‑AAG‑GCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAgcg       >  4:340533/1‑150 (MQ=255)

                                                                            t‑AAG‑GCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGcc     >  4:478867/1‑150 (MQ=255)

                                                                                g‑gCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCttt  >  2:1892905/1‑150 (MQ=255)

||

GCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAG‑GCGTTTA‑‑CCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTT  >  NC_000913_3_KO_pgi/4294728‑4294954

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`