New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_KO_pgi = 237489336 (0.990)5 (0.140) 5/280 4.4 12.7% coding (111/1356 nt) menE O‑succinylbenzoate‑CoA ligase
?NC_000913_3_KO_pgi = 2374914 33 (0.910)coding (90/1356 nt) menE O‑succinylbenzoate‑CoA ligase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CACCCGCGCCCCGCATTGCAGTAACGCCAGCCAGGCGAGCAGCGTTTGCGGCGTATTCCACGCCCGCAACATCACGCCGCTGCCCTCAACCACCCCCTGCACCGCAAATCCGGAGGCTAATTCATCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913_3_KO_pgi/2374767‑2374893
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gGCGCGAGCTTTGTGCTCGCGTCGAT  <  NC_000913_3_KO_pgi/2374914‑2374890
                                                                                                                                                        
CACCCGCGCCCCGCATTGCAGTAACGCCAGCCAGGCGAGCAGCGTTTGCGGCGTATTCCACGCCCGCAACATCACGCCGCGGCCCTCAACCACCCCCTGCACCGCAAATCCGGAGGCTAATTCATCGACGCGAGCTTTGTGCTCGCGTC     <  1:259921/149‑1
                       ACGCCAGCCAGGCGAGCAGCGTTTGCGGCGTATTCCACGCCCGCAACATCACGCCGCTGCCCTCAACCACCCCCTGCACCGCAAATCCGGAGGCTAATTCATCGACGCGAGCTTTGTGCTCGCGT      >  1:398187/1‑125
                       ACGCCAGCCAGGCGAGCAGCGTTTGCGGCGTATTCCACGCCCGCAACATCACGCCGCTGCCCTCAACCACCCCCTGCACCGCAAATCCGGAGGCTAATTCATCGACGCGAGCTTTGTGCTCGCGT      <  2:398187/125‑1
                                                                       TCACGCCGCTGCCCTCAACCACCCCCTGCACCGCAAATCCGGAGGCTAATTCATCGGCGCGAGCTTTGTGCTCGCGTCGAT  >  1:520988/1‑81
                                                                       TCACGCCGCTGCCCTCAACCACCCCCTGCACCGCAAATCCGGAGGCTAATTCATCGGCGCGAGCTTTGTGCTCGCGTCGAT  <  2:520988/81‑1
                                                                                                                                                        
CACCCGCGCCCCGCATTGCAGTAACGCCAGCCAGGCGAGCAGCGTTTGCGGCGTATTCCACGCCCGCAACATCACGCCGCTGCCCTCAACCACCCCCTGCACCGCAAATCCGGAGGCTAATTCATCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913_3_KO_pgi/2374767‑2374893
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gGCGCGAGCTTTGTGCTCGCGTCGAT  <  NC_000913_3_KO_pgi/2374914‑2374890

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.