New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_KO_pgi	= 4145334	23 (0.630)	7 (0.200)	5/272	4.3	23.3%	coding (1340/2298 nt)	pflD	putative glycine radical domain‑containing pyruvate formate‑lyase
	?	NC_000913_3_KO_pgi	= 4145346	24 (0.680)					coding (1352/2298 nt)	pflD	putative glycine radical domain‑containing pyruvate formate‑lyase
Rejected: Coverage evenness skew score above cutoff.

TCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAGGTTTACTGGAGCAGATCCGCGCCAAGATCAGCCACTACATCACCCTGATGGTTGAGGGCAGCAATATTTGTGATATCGGCCATCGCGACTGGGCACCTGTACCGCTGCTCTCATCGTTTAT  >  NC_000913_3_KO_pgi/4145199‑4145493                                                                                                                                                    |                                                                                                                                                    tCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGaa                                                                                                                                                    <  2:416498/149‑1 (MQ=255)       gTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAGATCTCCCTGCAAGAAAATGAAGTCAAt                                                                                                                                              >  2:131590/1‑149 (MQ=255)               tCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCACGACATCGCGAGGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCt                                                                                                                                      <  1:17176/149‑1 (MQ=255)                     gAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTTGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTa                                                                                                                                >  2:70039/1‑149 (MQ=255)                                 gACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGACGGTTTACt                                                                                                                    >  2:95222/1‑149 (MQ=255)                                  aCTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACTGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGCCCCCTGGCAACTGGAAAATGAAGGCAATGCTGCGCTGACTTATGAAGGTTTACTg                                                                                                                   <  1:316257/149‑1 (MQ=255)                                           tAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAGGt                                                                                                                         <  2:456359/134‑1 (MQ=255)                                           tAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAGGt                                                                                                                         >  1:456359/1‑134 (MQ=255)                                                      gtgGAATTATCTATTCCAGGCAGAACGTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGGAAGTGATGGGAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAGGTTTACTGGAGCAGATCCGCGCCAAAAt                                                                                               >  2:331732/1‑149 (MQ=255)                                                       tgGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAGGTTTACTGGAGCAGATCCGCGCCAAGATc                                                                                              <  1:242821/149‑1 (MQ=255)                                                       gggAATGATCTATTCCCGGCAGAACCTACGGCTTGCCTGACATCGCGATGTTTAATCTGCTTAAAGTGATGGAATTCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAGGTTTACTGGAGCAGATCCGCGCCAAGATc                                                                                              <  1:89300/148‑1 (MQ=255)                                                                cTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAGGTTTACTGGAGCAGATCCGCGCCAAGATCAGCCACTAc                                                                                     <  1:578048/149‑1 (MQ=255)                                                                   ttCCCGGCAGAACCTACGGCTTGCATGCCAAAACGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAGGTTTACTGGAGCAGATCCGCGCCAAGATCAGCCACTAGATc                                                                                  >  1:382337/1‑149 (MQ=255)                                                                   ttCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAGGTTTACTGGAGCAGATCCGCGCCAAGATCAGCCACTACATc                                                                                  >  2:560058/1‑149 (MQ=255)                                                                      ccGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAGGTTTACTGGAGCAGATCCGCGCCAAGATCAGCCACTACATCAcc                                                                               >  2:287703/1‑149 (MQ=255)                                                                                   cGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACACAGTGATTTTTACTGGAGCAGATCCGCGCCAAGATCAGCCACTACATCACCCTGATGGTTGAgt                                                                  >  1:471074/1‑148 (MQ=255)                                                                                       ttGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAGGTTTACTGGAGCAGATCCGCGCCAAGATCAGc                                                                                           <  2:275418/120‑1 (MQ=255)                                                                                       ttGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAGGTTTACTGGAGCAGATCCGCGCCAAGATCAGc                                                                                           >  1:275418/1‑120 (MQ=255)                                                                                                 tCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAGGTTTACTGGAGCAGATCCGCGCCAAGATCAGCCACTACATCACCCTGATGGTTGAGGGCAGCAATATTtgt                                                    <  2:620468/149‑1 (MQ=255)                                                                                                                    gAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAGGTTTACTGGAGCAGATCCGCGCCAAGATCAGCCACTACATCACCCTGATGGTTGAGGGCAGCAATATTTGTGATATCGGCCTTCGCGACt                                 >  1:565909/1‑149 (MQ=255)                                                                                                                             ggAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAGGTTTACTGGAGCAGATCCGCGCCAAGATCAGCCACTACATCACCCTGATGGTTGAGGGCAGCAATATTTGTGATATCGGCCATCGCGACTGGGcgactg                        >  2:257826/1‑144 (MQ=255)                                                                                                                             ggAAATCTGACTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAGGTTTACTGGAGCAGATCCGCGCCAAGATCAGCCACTACATCACCCTGATGGTTGAGGGCAGCAATATTTGTGATATCGGCCATCGCGACTGGGCACCTg                        <  1:214549/149‑1 (MQ=255)                                                                                                                                      cctggttGAAAATGAAGGGAGTGGTGCGGTTACTTATGAAGGTTTACTGGAGCAGATCCGCGCCAAGATCAGCCACTACATCACCCTGATGGTTGAGGGCAGCAATATTTGTGATATCGGCCATCGCGACTGGGCACCTGTACCgctgc               <  1:309343/143‑1 (MQ=255)                                                                                                                                           aTGAAAATGAAGGCAATGCTGCGCTGACTTATGAAGGTTTACTGGAGCAGATCCGCGCCAAGATCAGCCACTACATCACCCTGATGGTTGAGGGCAGCAATATTTGTGATATCGGCCATCGCGACTGGGCACCTGTACCGCTGCTCTCa          >  1:452547/1‑149 (MQ=255)                                                                                                                                                   gAAGGCAATGCTGCGCTGACTTATGACGGTTTGCTGGAGCCGATCCTCGCCAATATCAGCCACTACATCACACTGATGGTTGAGGGCAGCAGCTTTTATGATATCTGCCGTCGCGCCTGGGCCCCTCTACCGCTGCTCCCCTCGTTTAt  >  2:345668/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                    TCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAGGTTTACTGGAGCAGATCCGCGCCAAGATCAGCCACTACATCACCCTGATGGTTGAGGGCAGCAATATTTGTGATATCGGCCATCGCGACTGGGCACCTGTACCGCTGCTCTCATCGTTTAT  >  NC_000913_3_KO_pgi/4145199‑4145493

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.