New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_KO_pgi = 20103129 (0.800)5 (0.150) 5/266 4.2 14.8% coding (61/1026 nt) lpxD UDP‑3‑O‑(3‑hydroxymyristoyl)‑glucosamine N‑acyltransferase
?NC_000913_3_KO_pgi = 201041 30 (0.870)coding (71/1026 nt) lpxD UDP‑3‑O‑(3‑hydroxymyristoyl)‑glucosamine N‑acyltransferase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GCTTACAACAGCAGCGATGTAAAAGACATCACTGCCGACGTACTGAAACAGGTTAAATAAGTAATGCCTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGAACTACACGGTGATGGCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGCACAAACAGGTCACATTACGTTCATGGTTAACCCAAAATACCGTGAGCATTTAGGCTTGTGCCAGGCGTCCGCGGTTGTCATGACCCAGGACGATCTTCCTTTCGCGAAAAGTGCCGCA  >  NC_000913_3_KO_pgi/200908‑201189
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gCTTACAACAGCAGCGATGTAAAAGACATCACTGCCGACGTACTGAAACAGGTTAAATAAGTAATGCCTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGAACTACACGGTGATGGCGCTATCGTCATCACCGGCGTTGc                                                                                                                                       >  2:249594/1‑149 (MQ=255)
   tACAACAGCAGCGATGTAAAAGACATCACTGCCGACGTACTGAAACAGGTTAAATAAGTAATGCCTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGAACTACACGGTGATGGCGATATCGTCATCACCGGCGTTGCGTc                                                                                                                                    >  1:254532/1‑149 (MQ=255)
      aacaGCAGCGATGTAAAAGACATCACTGCCGACGTACTGAAACAGGTTAAATAAGTAATGCCTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGAACTACACGGTGATGGCGATATCGTCATCACCGGCGTTGCGTCCAt                                                                                                                                 <  2:217627/149‑1 (MQ=255)
              cGATGTAAAAGACATCACTGCCGACGTACTGAAACAGGTTAAATAAGTAATGCCTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGAACTACACGGTGATGGCGATATCGTCATCACCGGCGtt                                                                                                                                         <  2:561251/133‑1 (MQ=255)
              cGATGTAAAAGACATCACTGCCGACGTACTGAAACAGGTTAAATAAGTAATGCCTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGAACTACACGGTGATGGCGATATCGTCATCACCGGCGtt                                                                                                                                         >  1:561251/1‑133 (MQ=255)
                     aaaGACATCACTGCCGACGTACTGAAACAGGTTAAATAAGTAATGCCTTCAATTCGACTGGCTGATTTGGCGCGGCAGTTGGGTGCAGAACTACACGGTGATGGGTATATCGTCGTGACCGGCGTTGAGTCCATGCAATTTGCACAAAc                                                                                                                  >  2:299878/1‑149 (MQ=255)
                           aTCACTGCCGACGTACTGAAACAGTTTAAATAAGTAATGCCTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGCACTACACGGTGATGGCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGCACAAACAGGTca                                                                                                            <  2:270228/149‑1 (MQ=255)
                           aTCACTGCCGACGTACTGAAACAGGTTAAATAAGTAATGCCTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGAACTACACGGTGATGGCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGCACAAACAGCTca                                                                                                            >  2:121292/1‑149 (MQ=255)
                           aTCACTGCCGACGTACTGAAACAGGTTAAATAAGTAATGCCTTCAATTCGACAGGCTGATTTAGCGCAGCAGTTGGATGCAGAACTACACGGTGATGGCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGCACAAACAGGTca                                                                                                            >  2:599712/1‑149 (MQ=255)
                                tGCCGACGTACTGAAACAGGTTAAATAAGTAATGCCTTCAATTCGACTGGCTGATTTAGCGCGGCAGTTGGATGCAGAACTACACGGTGATGGCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGCACAAACAGGTCACATTa                                                                                                       >  1:320626/1‑149 (MQ=255)
                                tGCCGACGTACTGAAACAGGTTAAATAAGTAATGCCTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGAACTACACGGTGATGGCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGCACAAACAGGTCACATTa                                                                                                       >  1:368158/1‑149 (MQ=255)
                                         aCTGAAACAGGTTAAATAAGTAATGCCTGTAATTCGACTGGCTGATTTAGCGGAGCAGTTGGATGCAGAACTACACGGTGATGGCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGCACAAACAGGTCACATTACGTTCATgg                                                                                              <  2:33109/149‑1 (MQ=255)
                                           tGAAACAGGTTAAATAAGTAATGCCTTCAATTCGACTGGCTGATTTAGCGCGGCAGTTGGATGCAGAACTACACGGTGATGGCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGCACAAACAGGTCACATTACGTTCATGGtt                                                                                            <  2:320626/149‑1 (MQ=255)
                                           tGAAACAGGTTAAATAAGTAATGCCTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGAACTACACGGTGATGGCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTACACAAACAAGTCACATTACGTTCATgtta                                                                                            >  1:8034/1‑146 (MQ=255)
                                               aCAGGTTAAATAAGTAATGCCTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGAACTACATGGTGATGGCGATATCGTCATCACCGGCGTTGAGTCCATGCAATCTGCACAAACAGGTCACATTACGTTCATGGTTAAcc                                                                                        >  1:487845/1‑149 (MQ=255)
                                                    ttAAATAAGTAATGCCTTCAATCCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGAACTACACGGTGATGGCGATATCGTCATCACCGGCGTTGCGTCCAt                                                                                                                                 <  2:284871/103‑1 (MQ=255)
                                                    ttAAATAAGTAATGCCTTCAATCCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGAACTACACGGTGATGGCGATATCGTCATCACCGGCGTTGCGTCCAt                                                                                                                                 >  1:284871/1‑103 (MQ=255)
                                                         tAAGTAATGCCTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGAACTACACGGTGATGGCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGCACAAACAGGTCACATTACGTTCATGGTTAACCCAAAATACCg                                                                              <  1:536426/149‑1 (MQ=255)
                                                               aTGCCTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGAACTACACGGTGATGGCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGCACAAACAGGTCACATTACGTTCATGGTTAACCCAAAATACCGTGAGCa                                                                        >  2:552138/1‑149 (MQ=255)
                                                                                         accaccgcagATGGACGCAGAACGACACGGTCATGCCAATATCCTCAACACCGGCGTTGCGTCAATGCAATCTGCACAACCCGGTCACATTACGTTCATGGTTAACCCAAAATACCGTGAGCATTTAGGCTTGTGCCAGTCGACCGCgg                                              <  1:567892/143‑1 (MQ=255)
                                                                                           agcagcaGTTGGATGCAGAACTAAACGGTGATGGCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGCACAAACAGGTCACATTACGTTCATGGTTAACCCAAAATACCGTGAGCATTTAGGCTTGTGCCAGGCGTCCGCGGtt                                            <  2:350818/148‑1 (MQ=255)
                                                                                                cagTTGGATGCAGAACTACACGGTGATGGCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGCACAAACAGGTCACATTACGTTCATGGTTAACCCAAAATACCGTGAGCATTTAGGCTTGTGCCAGGCGTCCGCGGTTGTCAt                                       <  2:384512/149‑1 (MQ=255)
                                                                                                cagTTGGATGCAGAACTACACGGTGATGGCGATATAGTCATCAACGGCGTTGCGTCCATGCAATCTGCACAAACAGGTCACATTACGTTCATGGTTAACCAAAAATACCGTGAGAATTTAGGCTTGTGCGAGGCGTCGGCGGTTGTCAt                                       >  1:225356/1‑149 (MQ=255)
                                                                                                              aCTACACGGTGATGGCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGCACAAACAGGTCACATTACGTTCATGGTTAACCCAAAATACCGTGAGCATTTAGGCTTGTGCCAGGCGTCCGCGGTTGTCATGACCCAGGACGATc                         <  1:599712/149‑1 (MQ=255)
                                                                                                              aCTACACGGTGATGGCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGCACAAACAGGTCACATTACGTTCATGGTTAACCCAAAATACCGTGAGCATTTAGGCTTGTGCCAGGCGTCCGCGGTTGTCATGACCCAGGACGATc                         <  2:254532/149‑1 (MQ=255)
                                                                                                                tACACGGTGATGGCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGCACAAACAGGTCACATTACGTTCATGGTTAACCCAAAATACCGTGAGCATTTAGGCTTGTGCCAGGCGTCCGCTGTTGTCATGTCCCAGGACGATCtt                       <  1:552138/149‑1 (MQ=255)
                                                                                                                   acGGTGATGGCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGCACAAACAGGTCACATTACGTTCATGGTTAACCCAAAATACCGTGAGCATTTAGGCTTGTGCCAGGCGTCCGCGGTTGTCATGACCCAGGACGATCTTCCt                    >  1:396018/1‑149 (MQ=255)
                                                                                                                     ggTGATGGCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGCACAAACCGGTCCCTTTACGTTCATGGTTAACCCAAAATACCGTGAGCATTTAGGCTTGTGCCAGGCGTCCGCGGTTGTCATGACCCAGGACGATCTTCCttt                  <  1:531398/149‑1 (MQ=255)
                                                                                                                                    gTCATCACCGGCGTTGCGTCCATGCAATCTGCACAAACAGGTCACATTACGTTCATGGTTAACCCAAAATACCGTGAGCATTTAGGCTTGTGCCAGGCGTCCGCGGTTGTCATGACCCAGGACGATCTTCCTTTCGCGAAAAGTGCCGc   >  1:172549/1‑149 (MQ=255)
                                                                                                                                     tcatcaCCGGCGTTGCGTCCATGCAATCTGCACAAACAGGTCACATTACGTTCATGGTTAACCCAAAATACCGTGAGCATTTAGGCTTGTGCCAGGCGTCCGCGGTTGTCATGACCCAGGACGATGCTCCTTTCGCGAAAAGTGCCGCa  >  1:36303/1‑149 (MQ=255)
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GCTTACAACAGCAGCGATGTAAAAGACATCACTGCCGACGTACTGAAACAGGTTAAATAAGTAATGCCTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGAACTACACGGTGATGGCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGCACAAACAGGTCACATTACGTTCATGGTTAACCCAAAATACCGTGAGCATTTAGGCTTGTGCCAGGCGTCCGCGGTTGTCATGACCCAGGACGATCTTCCTTTCGCGAAAAGTGCCGCA  >  NC_000913_3_KO_pgi/200908‑201189

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.