| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913_3_pae_pgi | 3,440,176 | G→A | T285I (ACC→ATC) | rpoA ← | RNA polymerase, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913_3_pae_pgi | 3,440,176 | 0 | G | A | 96.0% | 63.0 / NA | 25 | T285I (ACC→ATC) | rpoA | RNA polymerase, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (7/17); minor base C (1/0); total (8/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.20e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTC > NC_000913_3_pae_pgi/3440034‑3440300 | aTCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTGCAGGCGTATGCCCAGAGACAGTCCACGGGCAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGATACGCt < 2:273753/149‑1 (MQ=255)aTCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGATACGCt > 1:23918/1‑149 (MQ=255) gggtACTCGGCAGGGATGCGTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGGCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGATACGCTGTAc < 2:280165/146‑1 (MQ=255) ggTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCCGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGATACGCTGTAc < 1:277499/149‑1 (MQ=255) ctCAGCGATGCTTGCCGGATGCCAGTTTCCCAGGCGCATTCCCATAGACAGTACACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGATACGCTGTACCAGATCAc < 1:30514/148‑1 (MQ=255) aTGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGTCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGCTTTAAGGAGCTCAACCTCGCTACGCTGTACCAGATCACCGctatc > 2:406426/1‑148 (MQ=255) tGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGATACGCTGTACCAGATCACCGATATAg < 2:238347/149‑1 (MQ=255) tGCTTCCCGGCGGCCCGTTTTCCCGGCGCATCCCCAGAGACTGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGCAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGATACGCTGTACCAGATCACCGATATAg < 1:86061/149‑1 (MQ=255) ggCCAGTTCTGCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGATACGCTGTACCAGATCACCGATATAGTGGATAGCTTc < 2:60365/149‑1 (MQ=255) cAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTGAGGCGTTTTAAGGAGCTCAACCTCGATACGCTGTACCAGATCACCGAGATAGTGGCTAGCTTCTGc > 1:13550/1‑149 (MQ=255) cAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGATACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGc > 2:94992/1‑149 (MQ=255) gcATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCGCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCCACACGACGTAGTGGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTa < 2:376980/149‑1 (MQ=255) aTGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTCGGCGTTTTAAGGAGCTCACCCTCGATCCGCTGTACCAGATCACCGATATATGGGATAGCTTCTGCTTTGAGGAGGTTAGc > 1:26768/1‑149 (MQ=255) cccAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGATACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCaga < 1:227391/149‑1 (MQ=255) cAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGATACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGc < 1:336737/149‑1 (MQ=255) agagACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGATACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCg < 2:376992/149‑1 (MQ=255) aGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGATACGCt < 1:46159/88‑1 (MQ=255) aGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGATACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGt < 2:261532/149‑1 (MQ=255) aGTCCACGGGAAACCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGATACGCt > 2:46159/1‑88 (MQ=255) aaGCCAGCACGTCTTTAATTTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGATACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGa < 2:399656/149‑1 (MQ=255) ccAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGATACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTg > 2:112198/1‑108 (MQ=255) ccAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGATACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTg < 1:112198/108‑1 (MQ=255) agagATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGATACGCTGTACCAGATCACCGCTATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGAATCAgggtccg > 1:260319/1‑147 (MQ=255) cAAGTTTAGGCGTTTTAAGGCGCTCACCCTCGACACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTggc < 2:110196/149‑2 (MQ=255) ggCGTTTTAAGGAGCTCAACCTCGATACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCtcttc < 1:232847/149‑1 (MQ=255) | ATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTC > NC_000913_3_pae_pgi/3440034‑3440300 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |