Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_pgi526,8000AG34.9% 105.0 / 64.0 87E1180E (GAA→GAG) rhsDRhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
Reads supporting (aligned to +/- strand):  ref base A (23/33);  new base G (15/15);  total (39/48)
Fisher's exact test for biased strand distribution p-value = 4.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.51e-01
Rejected as consensus: Frequency below/above cutoff threshold.

GCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCG‑GCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGCAGGGGCGGTATATTA  >  NC_000913_3_pae_pgi/526658‑526948
                                                                                                                                               |                                                                                                                                                    
gCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCG‑GCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTatgat                                                                                                                                                <  1:736558/149‑1 (MQ=255)
gCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCG‑GCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTatgat                                                                                                                                                <  2:561017/149‑1 (MQ=255)
     cTGACGGTGGAGCAACTGGCCAGACAGGGGGAGCCGGAATACACACCG‑GCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATg                                                                                                                                           <  1:243434/149‑1 (MQ=255)
      tGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCG‑GCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATgg                                                                                                                                          >  1:547783/1‑149 (MQ=255)
      tGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCG‑GCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATgg                                                                                                                                          <  1:58706/149‑1 (MQ=255)
      tGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCG‑GCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATgg                                                                                                                                          <  1:735312/149‑1 (MQ=255)
       gACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCG‑GCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATggg                                                                                                                                         <  1:482670/149‑1 (MQ=255)
       gACGGTGGAGCAACGGGCCAGACAGGTGGAGCCGGAATACACACCG‑GCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATggg                                                                                                                                         <  1:768461/149‑1 (MQ=255)
       gACGGCGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCG‑GCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATggg                                                                                                                                         <  1:308003/149‑1 (MQ=11)
          ggTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCG‑GCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCa                                                                                                                                      >  1:766039/1‑149 (MQ=255)
          ggTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCG‑GCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCa                                                                                                                                      >  1:276680/1‑149 (MQ=255)
          ggTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCG‑GCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCa                                                                                                                                      >  1:300970/1‑149 (MQ=255)
           gTGGAGCAACTGGCCAGACAGGTGGAGCCGGGATACACACCG‑GCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCaa                                                                                                                                     >  1:172874/1‑149 (MQ=255)
           gTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCG‑GCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATGCGGCGTGGCGCGGGGAGTATGATGAATGGGGCaa                                                                                                                                     <  1:162266/149‑1 (MQ=11)
           gTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCG‑GCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTatga                                                                                                                                                 <  1:284654/137‑1 (MQ=255)
           gTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCG‑GCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTatga                                                                                                                                                 >  2:284654/1‑137 (MQ=255)
           gTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCG‑GCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCaa                                                                                                                                     >  1:428415/1‑149 (MQ=255)
            tGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCG‑GCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAAc                                                                                                                                    <  1:315938/149‑1 (MQ=255)
            tGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCG‑GCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAAc                                                                                                                                    <  1:11741/149‑1 (MQ=255)
              gAGCAGCTGGCCAGACAGGTGGAGCCGGAATACACACCG‑GCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGGGTGGAGCGCGCAATCTGGTGAATGGGGCAACCa                                                                                                                                  >  1:884129/1‑149 (MQ=17)
              gAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCG‑GCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCa                                                                                                                                  <  1:530397/149‑1 (MQ=255)
              gAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCG‑GCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCCAAATCTGCAATACGGCGCGGCGCGGGGAGTATGATGAATGGGGCAACCa                                                                                                                                  >  1:947248/1‑149 (MQ=11)
                gCAACTGGCCAGACAGGTGGAGCCGGAATACACACCG‑GCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGc                                                                                                                                <  1:682214/149‑1 (MQ=255)
                gCAACTGGCCAGACAGGTGGAGCCGGAATACACACCG‑GCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGc                                                                                                                                <  1:682198/149‑1 (MQ=255)
                 cAACTGGCCAGACAGGTGGAGCCGGAATACACACCG‑GCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCt                                                                                                                               <  1:372878/149‑1 (MQ=255)
                 cAACTGGCCAGACAGGTGGAGCCGGAATACACACCG‑GCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTAATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCt                                                                                                                               <  2:943432/149‑1 (MQ=11)
                  aaCTGGCCAGACAGGTGGAGACGGAATACACACCG‑GCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGATGGCGATTATGAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCtt                                                                                                                              <  2:328173/149‑1 (MQ=11)
                      ggCCAGACAGGTGGAGCCGGAATACACACCG‑GCGCGCAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGCAAACGGCAACACGGCGTGGCGAGGGGAGCATGATGAATGGGGCGACCAGCTCAATg                                                                                                                          >  2:63635/1‑149 (MQ=11)
                       gCCAGAGAGGTGGAGCCGGAATACGCACCG‑GCGGGAAAGGCTCATCTTTATCCCTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGGGCGGAATATGATGAATGGGGCAACCAGCTTAATga                                                                                                                         <  2:87999/149‑1 (MQ=17)
                       gCCAGACAGGTGGAGCCGGAATACACACCG‑GCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGACGACGGGAATAAGGCGTGGCGCGGGGGGTATGATGGATGGGGCAACCAGCTCAGTga                                                                                                                         >  1:686965/1‑149 (MQ=11)
                       gCCAGACAGGTGGAGCCGGAATACACACCG‑GCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATga                                                                                                                         <  1:538102/149‑1 (MQ=255)
                       gCCAGACAGGTGGAGCCGGAATACACACCG‑GCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCAGCTGCAGCTTATCCGGGAAGGTTACGATACGTCTTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATga                                                                                                                         >  1:910435/1‑149 (MQ=11)
                        ccAGACAGGTGGAGCCGGAATACACTCCG‑GCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATgag                                                                                                                        <  1:956712/149‑1 (MQ=255)
                         cAGAGAGGGGGAGCGGGAGTA‑ACCCCGTGCGCAAAAAGGTCATCTTGTTCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATgagg                                                                                                                       <  2:402305/149‑1 (MQ=14)
                         cAGACAGGTGGAGCCGGAATACACACCG‑GCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGCTGAAGGGGGCAAGCAGCTTAATgaga                                                                                                                       >  1:466723/1‑148 (MQ=11)
                         cAGACAGGTGGAGCCGGAATACACACCG‑GCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATgagg                                                                                                                       >  1:17576/1‑149 (MQ=255)
                            aCAGGTGGAGCCGGAATACACACCG‑GCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGgaga                                                                                                                    >  1:188520/1‑149 (MQ=255)
                            aCAGGTGGAGCCGGAATACACACCG‑GCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCCGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGACGAAGGGGGCAACCAGGCGAGTGAGgaga                                                                                                                    >  2:97781/1‑149 (MQ=17)
                              aGGTGGAGCCGGAATACACACCG‑GCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAAc                                                                                                                  <  2:276680/149‑1 (MQ=255)
                              aGGTGGAGCCGGAATACACACCG‑GCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAAc                                                                                                                  <  1:897250/149‑1 (MQ=255)
                                 tGGAGCCGGAATACACACCG‑GCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCGAAATGAGGAAAACCCg                                                                                                               >  1:85816/1‑149 (MQ=255)
                                    aGCCGGAATGCACACCG‑GCGCGAAAATCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGTCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGcat                                                                                                            <  2:942737/149‑1 (MQ=11)
                                    aGCCGGAATACACACCG‑GCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGcat                                                                                                            <  1:231909/149‑1 (MQ=255)
                                     gCCGGAATACACACCG‑GCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGACGGAGGCGAAACGGCGCGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGcatc                                                                                                           >  2:833657/1‑149 (MQ=11)
                                        ggAATACACACCG‑GCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCAtg                                                                                                        <  2:188520/149‑1 (MQ=255)
                                         gAATACACACCG‑GCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGCAGACGGCAAGACGGCGTGGGGCGGGGAGGATGATGAATGGGGATACCAGCTGAATGAGGAGAACAGGcatagact                                                                                                       >  1:819435/1‑144 (MQ=11)
                                             acacacCG‑GCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTAt                                                                                                   <  1:628705/149‑1 (MQ=255)
                                               acacCG‑GCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATga                                                                                                                         >  1:308601/1‑125 (MQ=255)
                                               acacCG‑GCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATga                                                                                                                         <  2:308601/125‑1 (MQ=255)
                                               acacCG‑GCGCGAAAAGCTCATCTTTATCACTGCGACCACC‑GGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGCAGCGCGGGATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAg                                                                                                >  1:863270/1‑149 (MQ=255)
                                                 acCG‑GCGCGAAAAGCTCTTTTTTAGCACTGCGACAACGGGGGAGTGCCGCTGGCGCTTATCAGCGAGGACGGCAATACGGCGTGGAGGGCGGAATATGATGAAGGGGGCAACCAGCTTTATGAGGAGAACCCGCATCATGTGTATCAGc                                                                                               <  2:160356/149‑1 (MQ=255)
                                                  ccG‑GCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGcatcacctg                                                                                                      <  1:423037/141‑5 (MQ=11)
                                                  ccG‑GCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGcatcacctg                                                                                                      >  2:423037/1‑137 (MQ=255)
                                                      gcgcGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATAGGGGAACCCACGTAATGAGGAGGACCAGCAACAAATGTAGACGCCGTa                                                                                           >  2:588381/1‑149 (MQ=17)
                                                        gcgAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTAcc                                                                                         <  2:428415/149‑1 (MQ=255)
                                                        gcgAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCAGGCGCTTATCAGCGAACACGGCAATACGGCGGGGCGCGCGGAGTATGCTGAATGGGGCAACCAGCTTAGGAAGCAGACCCCGAGTCACCTGCACCAGCCGtcgc                                                                                         >  2:204838/1‑146 (MQ=255)
                                                          gAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGt                                                                                       >  1:224527/1‑149 (MQ=255)
                                                                 tCATCTTTATCCCTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACCGCGTGGAGCGCGGAGTATGATGAATGGGGCCACCACCTCACTGAGGAGAACCCGCAGCCTGTGTATCTGCCGTACCGTCTGCCAg                                                                                >  2:324496/1‑149 (MQ=17)
                                                                   aTCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGGGAAGACGGCGATCCGGCGTGAAGCGCGGAATATGATGAATGGGGCAAACAGCTTAATGAGGAGAACCCGCAACATGTGTATAAGCCGTACCGTCTGCCAgga                                                                              >  2:447517/1‑148 (MQ=18)
                                                                   aTCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATgag                                                                                                                        >  1:362140/1‑107 (MQ=255)
                                                                   aTCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATgag                                                                                                                        <  2:362140/107‑1 (MQ=11)
                                                                   aTCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAggg                                                                              <  1:523245/149‑1 (MQ=255)
                                                                     cTTCATCACTGCGACCACCGCAGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGgca                                                                            <  1:466586/149‑1 (MQ=255)
                                                                      gttATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACAGCGGGGAGCGCGGGATATGAAGAATGGGGCAACCAACTCAATGAGGAGAACCAGCAACATGTGTAACAGGAGAACAGACTGCCATGccag                                                                           >  2:257872/2‑149 (MQ=255)
                                                                        tATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGAGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGgcagca                                                                         <  1:128835/149‑1 (MQ=255)
                                                                            aCTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGGAGACGGCAGTACGGCGCGGCGCGGGGAGTAAGCGGAAAGGGGCAACCAGCTCAACGAGGAGAACCCGCACCCGCCGCACCAACCGTACCGTCCGCCGGGGACGCAGCat                                                                     >  2:875573/1‑149 (MQ=255)
                                                                            aCTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCat                                                                     >  1:348571/1‑149 (MQ=255)
                                                                              tGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCACCAGCatga                                                                   >  1:214889/1‑149 (MQ=255)
                                                                               gcaccgaccGGTGACTGTCGCTGGTGCGTATCAGCGAAGACGGCAATACGGCGTGGAGCGAGGAATAGGATGAATGGGGCAACCAGCTGAATGAGGAGAACCCGCAGCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCattat                                                                  <  2:555676/143‑1 (MQ=17)
                                                                                cGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCatgatg                                                                 <  1:785113/149‑1 (MQ=255)
                                                                                 gACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCAtgatga                                                                <  1:894079/149‑1 (MQ=255)
                                                                                   ccaccGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATatgatg                                                                                                                                               >  1:31347/1‑68 (MQ=255)
                                                                                   ccaccGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATatgatg                                                                                                                                               <  2:31347/68‑1 (MQ=255)
                                                                                    caccGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGa                                                             >  1:913192/1‑149 (MQ=255)
                                                                                     accGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCAGCCAGGGCAGCCGCATGATGCGGaa                                                            >  2:513882/1‑149 (MQ=255)
                                                                                       cGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTTTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGACGAGGAATc                                                          >  2:82702/1‑149 (MQ=255)
                                                                                           gACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGGGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAggg                                                      <  1:206490/149‑1 (MQ=255)
                                                                                            aCTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCat                                                                     >  1:380006/1‑133 (MQ=255)
                                                                                            aCTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCat                                                                     <  2:380006/133‑1 (MQ=255)
                                                                                             cTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGACGATGGTGAATGGGGAAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCt                                                    >  2:720658/1‑149 (MQ=255)
                                                                                                       cgcTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAAc                                          <  2:814464/149‑1 (MQ=255)
                                                                                                                     gACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGtacta                            <  2:172874/149‑1 (MQ=255)
                                                                                                                         gCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACggt                        >  2:21035/1‑147 (MQ=255)
                                                                                                                          cAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATc                       <  1:539690/149‑1 (MQ=255)
                                                                                                                                     ggAGCGGGGAATATGATGATTGGGGCACCCAGCTTAATGAGGAGAACCGGCATCATGTGTATGAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGGATCAGGGCGGTACTATAACCGTCACCGGTACTACGATCCGTTGCGgggg            <  2:127402/149‑1 (MQ=17)
                                                                                                                                        gcgcGGAATATGATGAATGGGGCAACCAGCTTGATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGCAGGGGCgg         <  2:348571/149‑1 (MQ=255)
                                                                                                                                            ggAAGATGAGGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGCAGGGGCGGtata     <  2:631813/149‑1 (MQ=255)
                                                                                                                                               atatGATGAATGGGGCAACCAGCTTAATGAGGAGAACCAGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGACGAGGAATCAGGGCAGTACTATAACCGTCACCGGTACTACGATCCGTTGCAGGGGCGGTATATTa  <  1:82702/149‑1 (MQ=255)
                                                                                                                                               atatGAGGAAGGGGGCAACCAGCTTAAGGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGCAGGGGCGGTATATTa  <  2:224527/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                    
GCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCG‑GCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGCAGGGGCGGTATATTA  >  NC_000913_3_pae_pgi/526658‑526948

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑