Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_pae_pgi	1,195,136	T→G	V5G (GTA→GGA)	icd →	isocitrate dehydrogenase; e14 prophage attachment site; tellurite reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_pae_pgi	1,195,136	0	T	G	100.0%	109.8 / NA	34	V5G (GTA→GGA)	icd	isocitrate dehydrogenase; e14 prophage attachment site; tellurite reductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (18/16);  total (18/16)

GTAGTATTGACAAGCCAATTACAAATCATTAACAAAAAATTGCTCTAAAGCATCCGTATCGCAGGACGCAAACGCATATGCAACGTGGTGGCAGACGAGCAAACCAGTAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGTAGTTGTTCCGGCACAAGGCAAGAAGATCACCCTGCAAAACGGCAAACTCAACGTTCCTGAAAATCCGATTATCCCTTACATTGAAGGTGATGGAATCGGTGTAGATGTAACCCCAGCCATGCTGAAAGTGGTCGACGCTGCAGTC  >  NC_000913_3_pae_pgi/1194995‑1195281                                                                                                                                              |                                                                                                                                                  gtagtaTTGACAAGCCAATTACAAATCATTAACAAAAAATTGCTCTAAAGCATCCGTATCGCAGGACGCAAACGCATATGCAACGTGGTGGCAGACGAGCAAACCAGTAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGGAgttgtt                                                                                                                                            <  2:262301/149‑1 (MQ=255)     taTTGACAAGCCAATTACAAATCATTAACAAAAAATTGCTCTAAAGCATCCGTCTCGCAGGACGCAAACGCATATGCAACGTGGTGGCAGACGAGCAAACCAGTAGCGCTCGAAGGAGCAGAGACTGGCAAGTACAGGAGTTGTTCCgg                                                                                                                                        >  2:148446/1‑149 (MQ=255)          aCAAGCCAATTACAAATCATTAACAAAAAATTGCTCTAAAGCATCCGTATGGCAGGAGGCAAACGAATATGCAACGTGGTGGCAGAGGAGCAAACCAGTGGCGCTGGAAGGAGAGGTGAATGGAAAGTAAAGGAGTTGTTCCGGCaaaa                                                                                                                                   >  1:37626/1‑149 (MQ=255)                                             caaaaGCATCCGTATCGCAGGACGCAAACGCATATGCAACGTGGTGGCAGACGAGCAAACCAGTAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGGAGTTGTTCCGGCACAAGGCAAGAAGATCACCCTGCAAAACGGCAAACTCaa                                                                                                <  2:316621/147‑1 (MQ=255)                                              tAAAGCATCCGTATCGCAGGACGCAAACGCATATGCAACGTGGTGGCAGACGAGCAAACCAGTAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGGAGTTGTTCCGGCACAAGGCAAGAAGATCACCCTGCAAAACGGCAAACTCAAc                                                                                               >  2:183824/1‑149 (MQ=255)                                                  gCATCCGTATCGCAGGACGCAAACGCATATGCAACGTGGTGGCAGACGAGCAAACCAGTAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGGAGTTGTTCCGGCACAAGGCCAGATAACCACCCTGCAAAACGGCAAACTCAACGTTc                                                                                           <  1:534936/149‑1 (MQ=255)                                                             gCAGGCCGCAAACGCATATGCAACGGGGTGGAAGACGAGCAAACCAGGAGCGGTCGAAGGAGAGGTGAATGGATAGTAAGGGAGTTGTTCCGTCACAAGGCAAGAAGATCACCCTGAAAAACGGCAAACTCAACGTTCCTGAAAATCCg                                                                                >  2:151755/1‑149 (MQ=255)                                                              ccaaaCGCAAAAGCATATGCAAAGCTGTGGCAGCGGAGCAAACCAGTAGCGCTCGAAGGAGAGGGGAATGGAAAGTAAAGGAGTTGTTCCGGCACAAGGCAAGCAGACGTCCGTGGAGCCCGTCAAACTCAACGTGCCTGAAAATCCgc                                                                               >  2:686549/5‑148 (MQ=255)                                                                      aaaCGCATATGCAACGTGGTGGCAGACGAGCAAACCAGTAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGGAGTTGTTCCGGCACAAGGCAAGAAGATCACCCTGCAAAACGGCAAACTCAACGTTCCTGAAAATCCGATTATCCCt                                                                       >  1:429756/1‑149 (MQ=255)                                                                       aaCGCATATGCAACGTGGGGGCAGACGAGCAAACCAGTAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGGAGTTGTTCCGGCACAAGGCAAGAAGATCACCCTGCAAAACGGCAAACTCAACGTTCCTGAAAATCCGATTATCCCtt                                                                      <  1:148446/149‑1 (MQ=255)                                                                        aCGCATATGCAACGTGGTGGCAGACGAGCAAACCAGTAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGGAGTTGTTCCGGCACAAGGCAAGAAGATCACCCTGCAAAACGGCAAACTCAACGTTCCTGAAAATCCGATTATCCCTTa                                                                     <  2:656436/149‑1 (MQ=255)                                                                            atatGCAACGTGGTGGCAGACGAGCAAACCAGTAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGGAGTTGTTCCGGCACAAGGCAAGAAGATCACCCTGCAAAACGGCAAACTCAACGTTCCTGAAAATCCGATTATCCCTTACAtt                                                                 >  1:74383/1‑149 (MQ=255)                                                                               tGCAACGTGGTGGCAGACGAGCAAACCAGTAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGGAGTTGTTCCGGCACAAGGCAAGAAGATCACCCTGCAAAACGGCAAACTCAACGTTCCTGAAAATCCGATTATCCCTTACATTGaa                                                              <  2:74383/149‑1 (MQ=255)                                                                                gCAACGTGGTGGCAGACGAGCAAACCAGTAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGGAGTTGTTCCGGCACAAGGCAAGAAGATCACCCTGCAAAACGGCAAACTCAACGTTCCTGAAAATCCGATTATCCCTTACATTGAAg                                                             >  1:38093/1‑149 (MQ=255)                                                                                 cAACGTGGTGGCAGACGAGCAAACCAGTAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGGAGTTGTTCCGGCACAAGGCAAGAAGATCACCCTGCAAAACGGCAAACTCAACGTTCCTGaaaa                                                                                    <  1:275137/125‑1 (MQ=255)                                                                                 cAACGTGGTGGCAGACGAGCAAACCAGTAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGGAGTTGTTCCGGCACAAGGCAAGAAGATCACCCTGCAAAACGGCAAACTCAACGTTCCTGaaaa                                                                                    >  2:275137/1‑125 (MQ=255)                                                                                         tggCAGACGAGCAAACCAGTAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGGAGTTGTTCCGGCACAAGGCAAGAAGATCACCCTGCAACACGGCAAACTCAACGTTCCTGAAAATCCGATTATCCCATACATTGAAGGTGATGGaa                                                    >  1:680091/1‑149 (MQ=255)                                                                                                  agaaaaCAAGGAGCGCTCGAAGGAGAGGTGAAGTGAAATGAAAGGAGTTGTGCCGGCACCAGGCAAGAAGATCACCCTGCCAAACGGCAAACGCAACGTTCCTGAAAATCCGATTATCCCTTACATTGAAGGTGATGGAATCGGTGTAg                                           <  2:501636/146‑1 (MQ=255)                                                                                                      aaCCAATAGCCCTCAAAAGAAAAGTGAATAAAACTTAAACGACTTTTTCCGGCACAAGGCAAGAAGATCACCCTGCAAAACGGCAAACTCAACGTTCCTGAAAATCCGATTATCCCTTACATTGAAGGTGATGGAATCGGTGTAGATGt                                       <  2:37626/149‑1 (MQ=255)                                                                                                       aCCAGTAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGGAGTTGTTCCGGCACAAGGCAAGAAGATCACCCTGCAAAACGGCAAACTCAACGTTCCTGAAAATCCGATTATCCCTTACATTGAAGGTGATGGAATCGGTGTAGATGTa                                      >  2:346948/1‑149 (MQ=255)                                                                                                              gcgcTCGAAGGAGAGGTGAATGGAAAGTAAAGGAGTTGTTCCGGCACAAGGCAAGAAGATCACCCTGCAAAACGGCAAACTCAACGTTCCTGAAAATCCGATTATcc                                                                         <  1:304050/107‑1 (MQ=255)                                                                                                              gcgcTCGAAGGAGAGGTGAATGGAAAGTAAAGGAGTTGTTCCGGCACAAGGCAAGAAGATCACCCTGCAAAACGGCAAACTCAACGTTCCTGAAAATCCGATTATcc                                                                         >  2:304050/1‑107 (MQ=255)                                                                                                              gcgcTCGAAGGAGAGGTGAATGGAAAGTAAAGGAGTTGTTCCGGCACAAGGCAAGAAGATCACCCTGCAAAACGGCAAACTCAACGTTCCTGAAAATCCGATTATCCCTTACATTGAAGGTGATGGAATCGgt                                               >  1:217852/1‑133 (MQ=255)                                                                                                              gcgcTCGAAGGAGAGGTGAATGGAAAGTAAAGGAGTTGTTCCGGCACAAGGCAAGAAGATCACCCTGCAAAACGGCAAACTCAACGTTCCTGAAAATCCGATTATCCCTTACATTGAAGGTGATGGAATCGgt                                               <  2:217852/133‑1 (MQ=255)                                                                                                              gcgcTCGAAGGAGAGCTAAACGGAAGGTAAATGAATTGTTCCGGCACAAGGCAAGAAGATCACCCTGCAAAACGTCAAACTCAACGTTCCTGAAAATCCGATTATCCCTTACATTGAAGGTGATGGAATCGGTGTAGATGTAACCCCAg                               <  2:323816/149‑1 (MQ=255)                                                                                                                     aaGGAGAGGTGAATGGAAAGTAAAGGAGTTGTTCCGGCACAAGGCAAGAAGATCACCCTGCAAAACGGCAAACTCAACGTTCCTGAAAATCCGATTAt                                                                           <  2:369395/98‑1 (MQ=255)                                                                                                                     aaGGAGAGGTGAATGGAAAGTAAAGGAGTTGTTCCGGCACAAGGCAAGAAGATCACCCTGCAAAACGGCAAACTCAACGTTCCTGAAAATCCGATTAt                                                                           >  1:369395/1‑98 (MQ=255)                                                                                                                      aGGAGAGGTGAATGGAAAGTAAAGGAGTTGTTCCGGCACAAGGCAAGAAGATCACCCTGCAAAACGGCAAACTCAACGTTCCTGAAAATCCGATTATCCCTTACATTGAAGGTGATGGAATCGGTGTAGATGTAACCCCAGCCATGCTg                       >  2:352558/1‑149 (MQ=255)                                                                                                                        gagaGGTGAATGGAAAGTAAAGGAGTTGTTCCGGCACAAGGCAAGAAGATCACCCTGCAAAACGGCAAACTCAACGTTCCTGAAAATCCGATTATCCCTTACATTGAAGGTGATGGAATAGATGTTCAAGCAACCCCAGCCATGCCGaa                     >  2:716881/1‑149 (MQ=255)                                                                                                                         agagGTGAAGGGAAAGTAAAGGAGTTGTTCCGGCACAAGGCAAGAAGATCACCCTGCAAAACGGCAAACTCAACGTTCCTGAAAATCCGATTATCCCTTACATTGAAGGTGATGGAATCGGTGTAGATGTAACCCCAGCCATGCTGaaa                    <  1:304789/149‑1 (MQ=255)                                                                                                                                  tGGAGAGTAAAGGAGTTGTTCCGGCACAAGGCAAGAAGATCACCCTGCAAATCGGCAAACTCAACGTTCCTGAAAATCCGATTATCCCTTACATTGAAGGTGATGGAATCGGTGTAGATGTAACCCCAGCCATGCTGAAAGTGGTCGAc           <  2:678553/149‑1 (MQ=255)                                                                                                                                  tGGAAAGTAAAGGAGTTGTTCCGGCACAAGGCAAGAAGATCACCCTGCAAAACGGCAAACTCAACGTTCCTGAAAATCCGa                                                                               <  2:643187/81‑1 (MQ=255)                                                                                                                                  tGGAAAGTAAAGGAGTTGTTCCGGCACAAGGCAAGAAGATCACCCTGCAAAACGGCAAACTCAACGTTCCTGAAAATCCGa                                                                               >  1:643187/1‑81 (MQ=255)                                                                                                                                   ggAAAGTAAAGGAGTTGTTCCGGGACAAGGCAAGAAGATCACACTCCAAAACGGCAAACTCAACGTTCCTGAAAATCCGATTATCCCTTACATTGAAGGTGATGGAATCGGTGTAGATGTAACCCCAGCCATGCTGAAAGTGGTCGACg          >  1:569464/1‑149 (MQ=255)                                                                                                                                           aaggaGTTGTTCCGGCACAAGGCAAGAAGATCACCCTGCAAAACGGCAAACTCAACGTTCCTGAAAATCCGATTATCCCTTACATTGAAGGTGATGGAATCGGTGTAGATGTAACCCCAGCCATGCTGAAAGTGGTCGACGCTGCAGTc  <  2:161047/149‑1 (MQ=255)                                                                                                                                           aaggaGTTGTTCCGGCACAAGGCAAGAAGATCAACCTGCAAAACGGCAAACTCAACGTTCCTGAAAATCCGATTATCCCTTACATTGAAGGTGATGGAATCGGTGTAGAt                                         <  2:304425/110‑1 (MQ=255)                                                                                                                                           aaggaGTTGTTCCGGCACAAGGCAAGAAGATCAACCTGCAAAACGGCAAACTCAACGTTCCTGAAAATCCGATTATCCCTTACATTGAAGGGGATGGAATCGGTGTAGAt                                         >  1:304425/1‑110 (MQ=255)                                                                                                                                           aaggaGTTGTTCCGGCACAAGGCAAGAACCTCACCCCACAAAGCCGCAAACTCAACGTTCCTGAAAATCCGATTATCCCTTACATTGAAGGTGATGGAATCGGTGTAGATGTAACCCCAGCCATGCTGAAAGTGGTCGACGCTGCAGTc  <  1:494031/149‑1 (MQ=255)                                                                                                                                              |                                                                                                                                                  GTAGTATTGACAAGCCAATTACAAATCATTAACAAAAAATTGCTCTAAAGCATCCGTATCGCAGGACGCAAACGCATATGCAACGTGGTGGCAGACGAGCAAACCAGTAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGTAGTTGTTCCGGCACAAGGCAAGAAGATCACCCTGCAAAACGGCAAACTCAACGTTCCTGAAAATCCGATTATCCCTTACATTGAAGGTGATGGAATCGGTGTAGATGTAACCCCAGCCATGCTGAAAGTGGTCGACGCTGCAGTC  >  NC_000913_3_pae_pgi/1194995‑1195281

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑