Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_pgi3,763,9750TC39.7% 54.6 / 80.8 78I598T (ATC→ACC) ‡rhsARhs protein with putative toxin 55 domain; putative polysaccharide synthesis/export protein; putative neighboring cell growth inhibitor
Reads supporting (aligned to +/- strand):  ref base T (26/21);  new base C (18/13);  total (44/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.51e-01
Rejected as consensus: Frequency below/above cutoff threshold.

TTGCCGTGAAAGACACGCAGGGCCATGAAACGCGGTATGAATACAACATCGCCGGTGACCTGACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTAACGCGCAGTATGGAATACGATGCTGCCGGACGGGTCATCCGCCTGAC  >  NC_000913_3_pae_pgi/3763927‑3764122
                                                |                                                                                                                                                   
ttGCCGTGAAAGACACGCAGGGCCATGAAACGCGGTATGAATACAACGCCGCCGGTGACCTGACCACCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTaa                                                <  1:453637/150‑1 (MQ=9)
 tGCCGTGAAAGACACGCAGGGCCATGAAACGCGGTATGAATACAACGCCGCCGGTGACCTGACCACCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTAAc                                               >  2:1501727/1‑150 (MQ=12)
 tGCCGTGAAAGACACGCAGGGCCATGAAACGCGGTATGAATACAACGCCGCCGGTGACCTGACCACCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTAAc                                               <  4:705621/150‑1 (MQ=12)
 tGCCGTGAAAGACACGCAGGGCCATGAAACGCGGTATGAATACAACGCCGCCGGTGACCTGACCACCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTAAc                                               >  2:593685/1‑150 (MQ=12)
 tGCCGTGAAAGACACGCAGGGCCATGAAACGCGGTATGAATACAACGCCGCCGGTGACCTGACCACCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTAAc                                               <  1:2072/150‑1 (MQ=12)
  gCCGTGAAAGACACGCAGGGCCATGAAACGCGGTATGAATACAACATCGCCGGTGACCTGACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTAAcg                                              <  4:47554/150‑1 (MQ=32)
   ccGTGAAAGACACGCAGGGCCATGAAACGCGGTATGAATACAACGCCGCCGGTGACCTGACCACCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTAAcgc                                             <  2:1651797/150‑1 (MQ=255)
   ccGTGAAAGACACGCAGGGCCATGAAACGCGGTATGAATACAACATCGCCGGTGACCTGACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGTGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTAAcgc                                             <  1:1201871/150‑1 (MQ=17)
   ccGTGAAAGACACGCAGGGCCATGAAACGCGGTATGAATACAACATCGCCGGTGACCTGACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTAAcgc                                             >  2:169409/1‑150 (MQ=32)
    cGTGAAAGACACGCAGGGCCATGAAACGCGGTATGAATACAACGCCGCCGGTGACCTGACCACCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTCGCACTCAGGGCGGGCTAAcgcg                                            >  1:14902/1‑150 (MQ=11)
     gTGAAAGACACGCAGGGCCATGAAACGCGGTATGAATACAACATCGCCGGTGACCTGACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTAACgcgc                                           >  1:1271934/1‑150 (MQ=32)
     gTGAAAGACACGCAGGGCCATGAAACGCGGTATGAATACAACATCGCCGGTGACCTGACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCCAACgcgc                                           >  4:137322/1‑150 (MQ=17)
      tGAAAGACACGCAGGGCCATGAAACGCGGTATGAATACAACGCCGCCGGTGACCTGACCACCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTAACGCGCa                                          >  2:66819/1‑150 (MQ=12)
      tGAAAGACACGCAGGGCCATGAAACGCGGTATGAATACAACATCGCCGGTGACCTGACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTAACGCGCa                                          <  4:572672/150‑1 (MQ=32)
          aGACACGCAGGGCCATGAAACGCGGTATGAATACAACGCCGCCGGTGACCTGACCACCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTAACGCGCAGTAt                                      >  4:567409/1‑150 (MQ=12)
          aGACACGCAGGGCCATGAAACGCGGTATGAATACAACATCGCCGGTGACCTGACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTAACGCGCAGTAt                                      >  2:1232524/1‑150 (MQ=32)
          aGACACGCAGGGCCATGAAACGAGGGATGAATACAACGCCGCCGGTGACCTGACCACCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTAACGCGCAGTAt                                      >  1:822507/1‑150 (MQ=11)
           gACACGCAGGGCCATGAAACGCGGTATGAATACAGCATCGCCGGTGACCTGACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTAACGCGCAGTATg                                     <  2:939116/150‑1 (MQ=17)
           gACACGCAGGGCCATGAAACGCGGTATGAATACAACGCCGCCGGTGACCTGACCACCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTAACGCGCAGTATg                                     >  4:51952/1‑150 (MQ=255)
           gACACGCAGGGCCATGAAACGCGGTATGAATACAACGCCGCCGGTGACCTGACCACCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTAACGCGCAGTATg                                     <  4:338081/150‑1 (MQ=12)
           gACACGCAGGGCCATGAAACGCGGTATGAATACAACATCGCCGGTGACCTGACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTAACGCGCAGTATg                                     <  1:169409/150‑1 (MQ=32)
           gACACGCAGGGCCATGAAACGCGGTATGAATACAACATCGCCGGTGACCTGACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTAACGCGCAGTATg                                     <  1:475126/150‑1 (MQ=32)
            acacGCAGGGCCATGAAACGCGGTATGAATACAACGCCGCCGGTGACCTGACCACCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGCAAGGCCGTCCGTACCACGCAGGGCGGGCTAACGCGCAGTATgg                                    >  4:973568/1‑150 (MQ=11)
            acacGCAGGGCCATGAAACGCGGTATGAATACAACATCGCCGGTGACCTGACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTAACGCGCAGTATgg                                    >  1:942302/1‑150 (MQ=32)
            acacGCAGGGCCATGAAACGCGGTATGAATACAACATCGCCGGTGACCTGACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTAACGCGCAGTACgg                                    >  4:272674/1‑150 (MQ=17)
             cacGCAGGGCCATGAAACGCGGTATGAATGCAACATCGCCGGTGACCTGACCGCCGTCAGGGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTAACGCGCAGTATGGa                                   <  4:944675/150‑1 (MQ=12)
             cacGCAGGGCCATGAAACGCGGTATGAATACAACGCCGCCGGTGACCTGACCACCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTAACGCGCa                                          <  4:312676/143‑1 (MQ=255)
             cacGCAGGGCCATGAAACGCGGTATGAATACAACGCCGCCGGTGACCTGACCACCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTAACGCGCa                                          >  3:312676/1‑143 (MQ=255)
             cacGCAGGGCCATGAAACGCGGTATGAATACAACATCGCCGGTGACCTGACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTAACGCGCAGTATGGa                                   >  4:666708/1‑150 (MQ=32)
              acGCAGGGCCATGAAACGCGGTATGAATACAACGCCGCCGGTGACCTGACCACCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTAACGCGCAGTATGGaa                                  >  1:996567/1‑150 (MQ=255)
              acGCAGGGCCATGAAACGCGGTATGAATACAACGCCGCCGGTGACCTGACCACCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTAACGCGCAGTATGGaa                                  <  4:593779/150‑1 (MQ=255)
                gCAGGGCCATGAAACGCGGTATGAATACAACGCCGCCGGTGACCTGACCACCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTAACGCGCAGTATGGAATa                                >  1:599806/1‑150 (MQ=12)
                gCAGGGCCATGAAACGCGGTATGAATACAACGCCGCCGGTGACCTGACCACCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTAACGCGCAGTATGGAATa                                >  3:4469/1‑150 (MQ=255)
                 cAGGGCCATGAAACGCGGTATGAATACAACATCGCCGGTGACCTGACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTAACGCGCAGTAt                                      >  1:704944/1‑143 (MQ=32)
                 cAGGGCCATGAAACGCGGTATGAATACAACATCGCCGGTGACCTGACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTAACGCGCAGTAt                                      <  2:704944/143‑1 (MQ=32)
                  ggggCCATGAAACGCGGTATGAATACAACGCCGCCGGTGACCTGACCACCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTAACGCGCAGTATGGAATACg                              <  2:574062/149‑1 (MQ=255)
                    ggCCATGAAACGCGGTATGAATACAACATCGCCGGTGACCTGACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTAACGCGCAGTATGGAATACGAt                            <  4:534445/150‑1 (MQ=32)
                     gCCATGAAACGCGGTATGAATACAACATCGCCGGTGACCTGACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTAACGCGCAGTATGGAATACGAtg                           <  1:1182181/150‑1 (MQ=32)
                      ccATGAAACGCGGTATGAATACAACATCGCCGGTGACCTGACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTAACGCGCAGTATGGAATACGAtgc                          >  3:115464/1‑150 (MQ=32)
                         tGAAACGCGGTATGAATACAACGCCGCCGGTGACCTGACCACCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTAACGCGCAGTATGGAATACGAtgctgc                       >  2:1064741/1‑150 (MQ=12)
                         tGAAACGCGGTATGAATACAACATCGCCGGTGACCTGACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTAACGCGCAGTATGGAATACGAtgctgc                       <  2:800636/150‑1 (MQ=32)
                         tGAAACGCGGTATGAATACAACATCGCCGGTGACCTGACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTAACGCGCAGTATGGAATACGAtgctgc                       >  1:393349/1‑150 (MQ=32)
                         tGAAACGCGGTATGAATACAACATCGCCGGTGACCTGACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTAACGCGCAGTATGGAATACGAtgctgc                       >  2:1449061/1‑150 (MQ=32)
                          gAAACGCGGTATGAATACAACATCGCCGGTGACCTGACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTAACGCGCAGTATGGAATACGATGCTGcc                      <  3:666708/150‑1 (MQ=32)
                           aaaCGCGGTATGAATACAACATCGCCGGTGACCTGACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTAACGCGCAGTATGGAATACGATGCTGCCg                     >  1:1045723/1‑150 (MQ=32)
                           aaaCGCGGTATGAATACAACATCGCCGGTGACCTGACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTAACGCGCAGTATGGAATACGATGCTGCCg                     <  2:1295952/150‑1 (MQ=32)
                           aaaCGCGGTATGAATACAACATCGCCGGTGACCTGACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTAACGCGCAGTATGGAATACGATGCTGCCg                     >  3:283026/1‑150 (MQ=32)
                             aCGCGGTATGAATACAACATCGCCGGTGACCTGACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTAACGCGCAGTATGGAATACGATGCTGCCGGa                   <  3:272674/150‑1 (MQ=32)
                               gcgGTATGAATACAACGCCGCCGGTGACCTGACCACCGTCATTGCCCCGGACGGCAGCCGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTAACGCGCAGTATGGAATACGATGCTGCCGGACg                 >  4:141394/1‑150 (MQ=255)
                                cgGTATGAATACAACGCCGCCGGTGACCTGACCACCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTAACGCGCAGTATGGAATACGATGCTGCCGGACgg                <  4:759363/150‑1 (MQ=12)
                                cgGTATGAATACAACGCCGCCGGTGACCTGACCACCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTAACGCGCAGTATGGAATACGATGCTGCCGGACgg                <  1:1320731/150‑1 (MQ=255)
                                 ggTATGAATACAACGCCGCCGGTGACCTGACCACCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTAACGCGCAGTATGGAATACGATGCTGCCGGACggg               <  1:140048/150‑1 (MQ=255)
                                 ggTATGAATACAACATCGCCGGTGACCTGACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTAACGCGCAGTATGGAATACGATGCTGCCGGACggg               <  4:868774/150‑1 (MQ=32)
                                  gTATGAATACAACGCCGCCGGTGACCTGACCACCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTAACGCGCAGTATGGAATACGATGCTGCCGGACGGGt              <  2:583448/150‑1 (MQ=12)
                                  gTATGAATACAACGCCGCCGGTGACCTGACCACCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTAACGCGCAGTATGGAATACGATGCTGCCGGACGGGt              >  2:1859/1‑150 (MQ=255)
                                  gTATGAATACAACATCGCCGGTGACCTGACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTAACGCGCAGTATGGAATACGATGCTGCCGGACGGGt              <  4:283026/150‑1 (MQ=32)
                                  gTATGAATACAACATCGCCGGTGACCTGACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGACGGGCTAACGCGCAGTATGGAATACGATGCTGCCGGACGGGt              >  1:493646/1‑150 (MQ=17)
                                   tATGAATACAACGCCGCCGGTGACCTGACCACCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTAACGCGCAGTATGGAATACGATGCTGCCGGACGGGTc             >  4:61886/1‑150 (MQ=255)
                                    aTGAATACAACGCCGCCGGTGACCTGACCACCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTAACGCGCAGTATGGAATACGATGCTGCCGGACGGGTCa            >  2:399812/1‑150 (MQ=255)
                                     tGAATACAACATCGCCGGTGACCTGACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTAACGCGCAGTATGGAATACGATGCTGCCGGACGGGTCAt           >  1:1640069/1‑150 (MQ=32)
                                     tGAATACAACATCGCCGGTGACCTGACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTAACGCGCAGTATGGAATACGATGCTGCCGGACGGGTCAt           >  3:1027537/1‑150 (MQ=32)
                                     tGAATACAACATCGCCGGTGACCTGACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGACGTAAGTACAAGAAAGAGCGGGATAAAGCGTAGTATGAGATAGGATGATGCCGGACGGGTAAt           >  1:1421144/1‑150 (MQ=11)
                                      gAATACAACGCCGCCGGTGACCTGACCACCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTAACGCGCAGTATGGAATACGATGCTGCCGGACGGGTCATc          >  2:1489833/1‑150 (MQ=255)
                                      gAATACAACATCGCCGGTGACCTGACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTAACGCGCAGTATGGAATACGATGCTGCCg                     <  1:337472/139‑1 (MQ=33)
                                      gAATACAACATCGCCGGTGACCTGACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTAACGCGCAGTATGGAATACGATGCTGCCg                     >  2:337472/1‑139 (MQ=33)
                                      gAATACAACATCGCCGGTGACCTGACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTAACGCGCAGTATGGAATACGATGCTGCCGGACGGGTCATc          >  2:394848/1‑150 (MQ=32)
                                       aaTACAACATCGCCGGTGACCTGACCGCCGTCATTGCCCCGCACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGACCGTCCGTACCACGCAGGGCGGGCTAACGCGCAGTATGGAATACGATGCTGCCGGACGGGTCATcc         >  4:612510/1‑150 (MQ=17)
                                         tACAACATCGCCGGTGACCTGACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTAACGCGCAGTATGGAATACGATGCTGCCGGACGGGTc             >  1:1629637/1‑144 (MQ=32)
                                         tACAACATCGCCGGTGACCTGACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTAACGCGCAGTATGGAATACGATGCTGCCGGACGGGTc             <  2:1629637/144‑1 (MQ=32)
                                         tACAACATCGCCGGTGACCTGACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTAACGCGCAGTATGGAATACGATGCTGCCGGACGGGTCATCCGc       <  1:1299253/150‑1 (MQ=32)
                                          acaacgCCGCCGGTGACCTGACCACCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTAACGCGCAGTATGGAATACGATGCTGCCGGACGGGTCATCCGcc      <  2:1335196/150‑1 (MQ=12)
                                            aacaTCGCCGGTGACCTGACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTAACGCGCAGTATGGAATACGATGCTGCCGGACGGGTCATCCGCCTg    >  3:122551/1‑150 (MQ=32)
                                            aacaTCGCCGGTGACCTGACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTAACGCGCAGTATGGAATACGATGCTGCCGGACGGGTCATCCGCCTg    >  4:896483/1‑150 (MQ=32)
                                            aacaTCGCCGGTGACCTGACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTAACGCGCAGTATGGAATACGATGCTGCCGGACGGGTCATCCGCCTg    >  3:256810/1‑150 (MQ=32)
                                            aacaTCGCCGGTGACCTGACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTAACGCGCAGTATGGAATACGATGCTGCCGGACGGGTCATCCGCCTg    >  3:100192/1‑150 (MQ=32)
                                            aacaTCGCCGGTGACCTGACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTAACGCGCAGTATGGAATACGATGCTGCCGGACGGGTCATCCGCCTg    >  4:154604/1‑150 (MQ=32)
                                              caTCGCCGGTGACCTGACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTAACGCGCAGTATGGAATACGATGCTGCCGGACGGGTCATCCGCCTGAc  <  2:1271934/150‑1 (MQ=32)
                                              caTCGCCGGTGACCTGACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTAACGCGCAGTATGGAATACGATGCTGCCGGACGGGTCATCCGCCTGAc  <  3:896483/150‑1 (MQ=32)
                                                |                                                                                                                                                   
TTGCCGTGAAAGACACGCAGGGCCATGAAACGCGGTATGAATACAACATCGCCGGTGACCTGACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTAACGCGCAGTATGGAATACGATGCTGCCGGACGGGTCATCCGCCTGAC  >  NC_000913_3_pae_pgi/3763927‑3764122

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑