New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009273 1681349 =34 (0.830)3 (0.080) 3/278 5.9 8.5% coding (1286/1647 nt) fumA fumarate hydratase (fumarase A), aerobic Class I
?CP009273 1681367 = 32 (0.820)coding (1268/1647 nt) fumA fumarate hydratase (fumarase A), aerobic Class I
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CCGTGTTTTTTACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCG  >  CP009273/1681202‑1681491
                                                                                                                                                   |                                                                                                                                              
ccgTGTTTTTTACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCCGTCGTTAAGCCAAGAGAACCGGAGGCATAACCTTc                                                                                                                                               >  2:220014/1‑149 (MQ=255)
      tttttACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTc                                                                                                                                               >  1:332863/1‑143 (MQ=255)
         ttACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTCCGGCGtttt                                                                                                                                      <  2:444006/149‑1 (MQ=255)
            cAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTCCGGCGTTTTGGc                                                                                                                                   <  2:370715/149‑1 (MQ=255)
             aGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGGCAAGAGAACCGGAGGCGTAACCTTCCGGCGTTTTGGcc                                                                                                                                  >  2:289500/1‑149 (MQ=255)
                cagccGTCGCCGGGTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTCCGGCGTTTTg                                                                                                                                     <  1:200555/140‑1 (MQ=255)
                       gcccTGCTGGCTGCGGTTTCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCAGTCCGGCGGTCGTTGGGCCACCATAACCGGAGGCATAACCTTCCGGCGTTTTGGCCGGAc                                                                                                                              <  1:452946/141‑1 (MQ=39)
                       cACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCgt                                                                                                                        <  2:553351/149‑1 (MQ=255)
                                           cTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAAGCGGATGATCTTTg                                                                                                    >  2:438666/1‑149 (MQ=255)
                                                gCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTa                                                                                               >  2:405376/1‑149 (MQ=255)
                                                 ccAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCGGGCGGTCGTTGGGCCAAGAGAACCGGCGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGGTGATCGTTg                                                                                                    >  1:77957/1‑143 (MQ=255)
                                                           ccttcTTCCACCCTACGCCTGCAGTTGATCGATATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGcc                                                                                    <  2:230546/145‑1 (MQ=255)
                                                            aTACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTCCGGCGTTTTGACCGGACCCGCGTAGTAAATCGGACGCTCTTTGATGTACTGCgg                                                                                         >  1:181755/1‑143 (MQ=255)
                                                            aTACTCCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCgg                                                                                         <  1:275335/143‑1 (MQ=255)
                                                                ttCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCa                                                                               >  2:634314/1‑149 (MQ=255)
                                                                ttCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCa                                                                               >  2:571253/1‑149 (MQ=255)
                                                                     cccTGCGCTTGCAGTGTATCGACATAATCACCCATCCGTCCGGCGGCCGTCCGGCCAAGAGAACCGGAGGCATAACCTTCCGGCGTTTGGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGtt                                                                          <  2:231968/149‑1 (MQ=37)
                                                                      ccTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCa                                                                               <  1:261267/143‑1 (MQ=255)
                                                                          cgcTTGCAGTTGACCGACATAAGACTCCATCCCGCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCTCCCGGCGTGTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGt                                                                           <  1:543708/143‑1 (MQ=37)
                                                                                 aGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGctc                                                              >  2:633618/1‑149 (MQ=255)
                                                                                       tCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCAATCCGccc                                                              >  1:334502/1‑141 (MQ=255)
                                                                                          aCATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCCCTCttt                                                           >  1:399576/1‑143 (MQ=255)
                                                                                            atcaGAATCCCTCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCa                                                         <  1:501271/140‑1 (MQ=255)
                                                                                              aaGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGACCTTTCAGTTTGGCg                                                 >  2:259146/1‑149 (MQ=255)
                                                                                                gAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTg                                               >  2:615005/1‑149 (MQ=255)
                                                                                                     cgtccgtccgTCGGTAGTTGGGACAAGAGAACTGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGt                                                >  1:456524/3‑143 (MQ=255)
                                                                                                      atccGTCCGGCGGTCGTTGGGCCAAGAGAACCGGCGGAATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCCat                                         >  2:169942/1‑149 (MQ=255)
                                                                                                             cggcggTCCTTGGGCCGAGAGAACCGGAGGCATAACCTTCCGGCGTTTTGGCCGGCCCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCtata                                        <  1:402156/143‑1 (MQ=255)
                                                                                                              ggcggTCGTTGGGTCAAGAGAACCGGAGGCATACCCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCGTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGTGCAATATCAcgac                                 <  2:503584/149‑1 (MQ=255)
                                                                                                                               gagaACCGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGCCGATaaac                      >  1:407391/1‑141 (MQ=255)
                                                                                                                                     cGGAGGCATGACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCg                <  1:417181/143‑1 (MQ=255)
                                                                                                                                        aGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTTTATCGGGTGATCTTTGATGTACTGCGGCAGCCCTTCTCCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTa             <  1:122259/143‑1 (MQ=255)
                                                                                                                                            aTAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAACCGTGCAGTTAAGCg         >  1:364746/1‑143 (MQ=255)
                                                                                                                                             tAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCCCGACCGACGATAATCCTGCCGTTAACCGATAAGCg  >  2:311735/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                              
CCGTGTTTTTTACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCG  >  CP009273/1681202‑1681491

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.