New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	CP009273	1681349 =	34 (0.830)	3 (0.080)	3/278	5.9	8.5%	coding (1286/1647 nt)	fumA	fumarate hydratase (fumarase A), aerobic Class I
	?	CP009273	1681367 =	32 (0.820)					coding (1268/1647 nt)	fumA	fumarate hydratase (fumarase A), aerobic Class I
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGAT  >  CP009273/1681225‑1681505                                                                                                                                               |                                                                                                                                           gcccTGCTGGCTGCGGTTTCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCAGTCCGGCGGTCGTTGGGCCACCATAACCGGAGGCATAACCTTCCGGCGTTTTGGCCGGAc                                                                                                                                            <  1:452946/141‑1 (MQ=39) cACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCgt                                                                                                                                      <  2:553351/149‑1 (MQ=255)                     cTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAAGCGGATGATCTTTg                                                                                                                  >  2:438666/1‑149 (MQ=255)                          gCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTa                                                                                                             >  2:405376/1‑149 (MQ=255)                           ccAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCGGGCGGTCGTTGGGCCAAGAGAACCGGCGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGGTGATCGTTg                                                                                                                  >  1:77957/1‑143 (MQ=255)                                     ccttcTTCCACCCTACGCCTGCAGTTGATCGATATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGcc                                                                                                  <  2:230546/145‑1 (MQ=255)                                      aTACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTCCGGCGTTTTGACCGGACCCGCGTAGTAAATCGGACGCTCTTTGATGTACTGCgg                                                                                                       >  1:181755/1‑143 (MQ=255)                                      aTACTCCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCgg                                                                                                       <  1:275335/143‑1 (MQ=255)                                          ttCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCa                                                                                             >  2:634314/1‑149 (MQ=255)                                          ttCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCa                                                                                             >  2:571253/1‑149 (MQ=255)                                               cccTGCGCTTGCAGTGTATCGACATAATCACCCATCCGTCCGGCGGCCGTCCGGCCAAGAGAACCGGAGGCATAACCTTCCGGCGTTTGGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGtt                                                                                        <  2:231968/149‑1 (MQ=37)                                                ccTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCa                                                                                             <  1:261267/143‑1 (MQ=255)                                                    cgcTTGCAGTTGACCGACATAAGACTCCATCCCGCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCTCCCGGCGTGTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGt                                                                                         <  1:543708/143‑1 (MQ=37)                                                           aGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGctc                                                                            >  2:633618/1‑149 (MQ=255)                                                                 tCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCAATCCGccc                                                                            >  1:334502/1‑141 (MQ=255)                                                                    aCATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCCCTCttt                                                                         >  1:399576/1‑143 (MQ=255)                                                                      atcaGAATCCCTCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCa                                                                       <  1:501271/140‑1 (MQ=255)                                                                        aaGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGACCTTTCAGTTTGGCg                                                               >  2:259146/1‑149 (MQ=255)                                                                          gAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTg                                                             >  2:615005/1‑149 (MQ=255)                                                                               cgtccgtccgTCGGTAGTTGGGACAAGAGAACTGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGt                                                              >  1:456524/3‑143 (MQ=255)                                                                                atccGTCCGGCGGTCGTTGGGCCAAGAGAACCGGCGGAATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCCat                                                       >  2:169942/1‑149 (MQ=255)                                                                                       cggcggTCCTTGGGCCGAGAGAACCGGAGGCATAACCTTCCGGCGTTTTGGCCGGCCCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCtata                                                      <  1:402156/143‑1 (MQ=255)                                                                                        ggcggTCGTTGGGTCAAGAGAACCGGAGGCATACCCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCGTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGTGCAATATCAcgac                                               <  2:503584/149‑1 (MQ=255)                                                                                                         gagaACCGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGCCGATaaac                                    >  1:407391/1‑141 (MQ=255)                                                                                                               cGGAGGCATGACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCg                              <  1:417181/143‑1 (MQ=255)                                                                                                                  aGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTTTATCGGGTGATCTTTGATGTACTGCGGCAGCCCTTCTCCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTa                           <  1:122259/143‑1 (MQ=255)                                                                                                                      aTAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAACCGTGCAGTTAAGCg                       >  1:364746/1‑143 (MQ=255)                                                                                                                       tAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCCCGACCGACGATAATCCTGCCGTTAACCGATAAGCg                >  2:311735/1‑149 (MQ=255)                                                                                                                            gtccggccaaaacgCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAg           >  2:89072/14‑149 (MQ=255)                                                                                                                               cGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGGGTGTAGaac        >  2:423458/1‑148 (MQ=255)                                                                                                                                 gCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATGACGACCGCCGCTAATCGTGCCCTTAGGCGATAAGCGTGTa            >  1:556961/1‑143 (MQ=37)                                                                                                                                    ttttGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGaa         >  1:408630/1‑143 (MQ=255)                                                                                                                                       tGGCTGGACCCGCTTAGTAAATCGGATTATCTTTGATGTACTGCGGCAGCCCTTCACCGTTAGCCATCCGCCCTTGCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACg      <  1:11080/143‑1 (MQ=255)                                                                                                                                           cGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGAt  <  1:363983/143‑1 (MQ=255)                                                                                                                                               |                                                                                                                                           CACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGAAACGGGAT  >  CP009273/1681225‑1681505

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.