New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009273 3338351 =55 (1.350)4 (0.110) 4/270 5.2 7.8% coding (276/1434 nt) rpoN RNA polymerase, sigma 54 (sigma N) factor
?CP009273 3338383 = 44 (1.160)coding (308/1434 nt) rpoN RNA polymerase, sigma 54 (sigma N) factor
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TAATCCGCTGCTTGAGCAAATCGACACTCATGAAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGACGAGCTGCCGGTCTACCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTTGAGCTGACACCGTTTTCCGACACTGACCGCGCTATT  >  CP009273/3338204‑3338495
                                                                                                                                                    |                                                                                                                                                
tAATCCGCTGCTTGAGCAAATCGACACTCATGAAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTaca                                                                                                                                                 >  2:686828/1‑149 (MQ=255)
tAATCCACTGCTTGAGCAAATCGACACTCATGAAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTaca                                                                                                                                                 >  2:280432/1‑149 (MQ=255)
   tCCGCTGCTTGAGCAAATCGACACTCATGAAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGAACGAAGAGAAATAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCg                                                                                                                                              >  2:164070/1‑149 (MQ=255)
           ttGAGCAAATCGACACTCATGAAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGCAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTaca                                                                                                                                      <  2:503243/149‑1 (MQ=255)
            tGAGCAAATCGACACTCATGAAGAAATCGACACCGGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTg                                                                                                                                           >  1:454341/1‑143 (MQ=255)
               gCAAATCGACGCTCATGAAGAACTCGACCCCCGCGCAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTa                                                                                                                                        <  1:156371/143‑1 (MQ=255)
                cAAATCGACACTCATGAAGAAATCGGCACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGCCGCGCGCGAACACAAAGAGATGCCGGAAGAGCTGCCACTCGATGCAAGTTGGGACACCATATAAACCGCTGGtcc                                                                                                                                       >  1:8703/1‑141 (MQ=255)
                cAAATCGACACTCATGAAGAAATCGACACACGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACTCCATTTACACCGCTGGTACACCATc                                                                                                                                 >  2:427665/1‑149 (MQ=255)
                 aaaTCGACACTCATGAAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGACCACACAGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTaca                                                                                                                                      <  1:551466/143‑1 (MQ=255)
                 aaaTCGACACTCATGAAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTaca                                                                                                                                      <  1:405128/143‑1 (MQ=255)
                   aTCGACACTCATGAAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACAcc                                                                                                                                    >  1:491831/1‑143 (MQ=255)
                      gACACTCATGAAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTCCACAGCTGGTACACCATc                                                                                                                                 >  1:114306/1‑143 (MQ=255)
                             aTGAAGACATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGt                                                                                                                    <  2:297399/149‑1 (MQ=255)
                             aTGAAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCAcc                                                                                                                          >  1:90832/1‑143 (MQ=255)
                             aTGAAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGt                                                                                                                    >  2:27811/1‑149 (MQ=255)
                             aTGAAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGt                                                                                                                    <  2:344481/149‑1 (MQ=255)
                             aTGAAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGt                                                                                                                    <  2:693562/149‑1 (MQ=255)
                               gaagaaATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAg                                                                                                                        >  1:521513/1‑143 (MQ=255)
                                 agaaATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGGACACCATCCGGCACCAGCg                                                                                                                      >  1:274246/1‑143 (MQ=255)
                                 acaaaTCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGc                                                                                                                       <  1:563243/141‑1 (MQ=255)
                                     aTCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACAt                                                                                                            <  2:123146/149‑1 (MQ=255)
                                      tCGACCCCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCTGCAAGAGCCGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGAc                                                                                                                 <  1:39964/143‑1 (MQ=255)
                                      tCGACACCCGCGAAACGCAAGACAGCGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGCAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGAc                                                                                                                 >  1:230778/1‑143 (MQ=255)
                                            ccccCGAAACGCAAGNCAGNGNANCNCTGGACATCGACGATGCGCTCGAAGAAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTgacgac                                                                                                     <  2:478493/149‑1 (MQ=255)
                                            cccGCGAAACGCAAGACAGTTAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTgacgac                                                                                                     <  2:462338/149‑1 (MQ=255)
                                                      gccaGACAGTGAAACGCTGGACACCGCCGCCGCGCTCGAACAAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGACGAGCTGCCgg                                                                                           <  2:240052/146‑1 (MQ=255)
                                                       cAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGACGAggt                                                                                                >  1:373739/1‑141 (MQ=255)
                                                        aaGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGACGAGCTGCCGGTc                                                                                         <  2:540427/149‑1 (MQ=255)
                                                         aGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGACGAGCTGc                                                                                              <  1:88670/143‑1 (MQ=255)
                                                            cAGTGAAACGCTGGACACCGCCGACGCGCTCGGACACAAAGAGATGCCGGAAGGGCTGCCGCTCGATGCGAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCAGCCGCGGTGACTACATTGACGACGAGCTGCCGGTCTagc                                                                                     <  2:328290/149‑3 (MQ=255)
                                                             aGTGCAACGCCGGACACCGGCGACGGGCCCGGGCGAAAAAAGATGCCGGAGCGTCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGACGAGCTGCCGGTCTACCa                                                                                    <  2:270494/149‑1 (MQ=255)
                                                              gTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACATCATTTCAACCGCTTGTAGGTAGCTAAACACCAGCGGTGACTACATTGACGACGAGCTGCCGGTCTACCAg                                                                                   <  2:342721/149‑1 (MQ=255)
                                                                gacaCGCTGGACACCGCCGACGCGCGCGAACAAAACGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGACGAGCTGCCGGTCTACCAggg                                                                                 <  2:220927/146‑1 (MQ=255)
                                                                gAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCAGGCACCAGCGGTGACTACATTGAGGACGAGCTGCCGGTCTACCCggg                                                                                 >  2:558215/1‑149 (MQ=255)
                                                                              cgccgACGCGCTCGAACAAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGACGAGCTGCCGGTCTACCAGGGCGAAACGACGCAGa                                                                   >  2:454132/1‑149 (MQ=255)
                                                                                  caCGCGCTCGAACAAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGACGAGCTGCCGGTCTACCAGGGCGAAACGACGCAGACCtt                                                               <  2:49408/148‑1 (MQ=255)
                                                                                   aCGCGCTCGAACAAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGACGAGCTGCCGGTCTACCAGGGCGAAACGACGCAg                                                                    >  1:532470/1‑143 (MQ=255)
                                                                                    ggcgcTCGAACAAAAAGAGATGCCGGAAGAGCTGCAGCTCGATGCCAGTTGGGAAACCATTTACACCGCTGGGCCACCATCCGGCACCAGCGGTGGCTACATTGACGCAGAGCTGCCGGTCTCCACGGGCGAAacggacaaca                                                                   >  1:112695/2‑136 (MQ=255)
                                                                                      cgcTCGAACAAGAAGAGAGGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGACGAGCTGCCGGTCCACCAGGGCGAAACGACGCAGAcc                                                                 >  1:536686/1‑143 (MQ=255)
                                                                                        cTCGAACAAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGACGAGCTGCCGGTCTACCAGGGCGAAACGACGCAGACCTTGCAGGa                                                         <  2:421906/149‑1 (MQ=255)
                                                                                                        aTGCCGGAAGAGCTGCCGCTCGATGCACGTTGGGTCACCACTTACTCCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGACGAGCTGCCGGTCTACCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCa                                          <  2:306900/149‑1 (MQ=255)
                                                                                                          gCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGACGAGCTGCCGGTCTACCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAgg                                        >  2:689252/1‑149 (MQ=255)
                                                                                                              gAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGACGAGCTGCCGGTCTACCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCa                                          <  1:546974/143‑1 (MQ=255)
                                                                                                                    cTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGACGAGCTGCCGGTCTACCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTTGAGCTGac                              <  2:642810/149‑1 (MQ=255)
                                                                                                                       ccGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGACGAGCTGCCGGTCTACCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTTGAGCt                                 >  1:476893/1‑143 (MQ=255)
                                                                                                                         gCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGACGAGCTGCCGGTCTACCAGGGCGAAACGCCGCAGACCTTGCAGGATTACCTGATGTGGCAGGTTGAGCTGGCACCGt                         >  2:457114/1‑149 (MQ=255)
                                                                                                                         gCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGACGAGCTGCCGGTCTACCAGGGCGAAACGACGCAGACCTTGCAGGATTCCCTGATTTGGCCGGTTGAGCTgc                               >  1:48915/1‑142 (MQ=255)
                                                                                                                          cccgctGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGACGAGCTGCCGGTCTACCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTTGAGCTGACACCGtt                        <  2:264592/144‑1 (MQ=255)
                                                                                                                                 ccAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGACGAGCTGCCGGTCTACCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTTGAGCTGACACCGTTTTCCGac                 <  2:543007/149‑1 (MQ=255)
                                                                                                                                      tGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGACGAGCTGCCGGTCTACCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTTGAGCTGACACCGTTTTCCGa                  <  1:384559/143‑1 (MQ=255)
                                                                                                                                           cacCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGACGAGCTGCCGGTCTACCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTTGAGCTGCCACCGGTTTCCGACCCTGACcgcg       >  2:597027/1‑149 (MQ=255)
                                                                                                                                            acCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGACGAGCTGCCGGTCTACCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTTGAGCTGACGCCGTTTTCCGACACTGACCgcgc      >  2:58368/1‑149 (MQ=255)
                                                                                                                                                tttACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGACGAGCTGCCGGTCTACCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTTGAGCTGACACCGTTTTCCGACACTGACCGCGCTAtt  <  2:122747/149‑1 (MQ=255)
                                                                                                                                                tttACACCGCTGGTACACCATCCGGCACAAGCGGTGACTACATTGACGATGAGCTGCCGGTCTACCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTTGAGCTGCCACCGTTTTCCGCCACTGACcgc        >  1:622157/1‑143 (MQ=255)
                                                                                                                                                  cacacCCCTGGTACACCATCCCGCACCAGCGGTGACTACATTGCCGACGACCTGCCGGTCTACCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCATGTTGAGCTGACACCGTTTTCCGACACTGACCgcgc      <  1:166175/142‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
TAATCCGCTGCTTGAGCAAATCGACACTCATGAAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGACGAGCTGCCGGTCTACCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTTGAGCTGACACCGTTTTCCGACACTGACCGCGCTATT  >  CP009273/3338204‑3338495

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.