Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	CP009273	2,420,338	+AATT	intergenic (‑71/+150)	hisJ ← / ← argT	histidine/lysine/arginine/ornithine transporter subunit/lysine/arginine/ornithine transporter subunit

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	CP009273	= 2420334	1 (0.020)	47 (0.810) +AATT	42/276	0.3	97.9%	intergenic (‑67/+154)	hisJ/argT	histidine/lysine/arginine/ornithine transporter subunit/lysine/arginine/ornithine transporter subunit
	?	CP009273	2420335 =	1 (0.020)					intergenic (‑68/+153)	hisJ/argT	histidine/lysine/arginine/ornithine transporter subunit/lysine/arginine/ornithine transporter subunit

TTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  CP009273/2420188‑2420334 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑AATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAG  >  CP009273/2420335‑2420480                                                                                                                                                    ||||                                                                                                                                                   TTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTAACATAAAA                                                                                                                                                      <  2:412284/149‑1 TTTTGCGGAACCGCAGCAAACGCGGCAGTTGCGCTGGAGAAGGCCATAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTAACATAAAA                                                                                                                                                      <  2:3831/149‑1               AGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTAACATAAAATTAATTGACCATCA                                                                                                                                        >  2:589174/1‑149                 CAAACGCAGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTAAACTTACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTAACATAAAATTAATTGACCATCAAG                                                                                                                                      <  2:1029492/149‑1                      GCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTAACATAAAATTAATTGACCATCACGGCAAA                                                                                                                                 >  2:259076/1‑149                           AGTTGCGCTGGATGAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTAACATAAAATTAATTGACCATCAAGGCAAAATATC                                                                                                                            <  2:815267/149‑1                                  CTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTAACATAAAATTAATTGACCATCAAGGCAAAATATCCCGCCCC                                                                                                                     >  2:599737/1‑149                                         AGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTAACATAAAATTAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGAC                                                                                                              <  2:780472/149‑1                                           GCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTAACATAAAATTAATTGACCATCAAGGCAAAATATCCCGCCCCGTTA                                                                                                                 <  1:236810/144‑1                                            CCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTAACATAAAATTAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTA                                                                                                           <  2:883406/149‑1                                             CAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTAACATAAAATTAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAaa                                                                                                               >  1:15420/1‑142                                                  CCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTAACATAAAATTAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTT                                                                                                     >  2:946353/1‑149                                                            CGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTCTGTTACACTTATCGTAACATAAAATTAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCAT                                                                                                >  1:201235/1‑144                                                             GATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTAACATAAAATTAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATT                                                                                               <  1:380648/144‑1                                                              ATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTTTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTAACATAAAATTAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAAT                                                                                         <  2:846249/149‑1                                                                   ACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATGTTTACAGGTTTGTAACACTTATCGTAACATAAAATTAATTGACCATCAAGGCAAAATATTCCGCCCCGTTAGACGTATTATTTTGCATTAAAAATGCTTA                                                                                    >  2:971736/1‑149                                                                             TCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTAACATAAAATTAATTGACAATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTAT                                                                               <  1:333059/144‑1                                                                               ATAATGTCCTCAACTGACAGTCTTTGTATAGGGAATATTTACAGGTTTGTTACACTTATCGTAACATAAAATTAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTC                                                                        >  2:533718/1‑149                                                                                 AATGTCCTCACCTGACAGTCTTTTTAAAGGAAATATTTACAGGTTTGTTACACTTATCGTAACATAAAATTAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAA                                                                      <  2:17472/149‑1                                                                                 AATGTCCTCAACGTACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTAACATAAAATTAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCAT                                                                           <  1:536418/144‑1                                                                                     TCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTAACATAAAATTAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCA                                                                  >  2:41680/1‑149                                                                                     TCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTAACATAAAATTAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCA                                                                       >  1:934477/1‑144                                                                                     TCCTCAACTGACAGTATTTTTATAGGGAATATTTCCAGGTTTGTTACACTTATCGTAACATAAAATTAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCA                                                                       <  1:473813/144‑1                                                                                      CCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTAACATAAAATTAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCAC                                                                 <  2:639227/149‑1                                                                                        TCAACTGACAGTCTTTTTATAGGGAATACAACAAGAGAAGTTACACTTATCGTAACATAAAATTAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCA                                                               >  2:1020430/1‑149                                                                                          AACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTAACATAAAATTAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACaccg                                                             >  2:477908/1‑145                                                                                             TGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTAACATAAAATTAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAAGGCTTAATTATGCATTTCAATCAACCACGATA                                                          >  2:51274/1‑149                                                                                                CAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTAACATAAAATTAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTG                                                       <  2:68270/149‑1                                                                                                CAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTAACATAAAATTAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTG                                                       >  2:368396/1‑149                                                                                                  tTCTTTTGATAGGGAATATTTACAGGTTTGTTACACTTATCGTAACATAAAACTAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATA                                                          <  1:181817/143‑1                                                                                                  GTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTAGCGTAACATAAAATTAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCA                                                     <  2:810206/149‑1                                                                                                     TTTTTAGAGGGAATATTTACAGGTTTGTTACACTTATCGTAACATAAAATTAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTAATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTG                                                       >  1:941215/1‑144                                                                                                       TTTATATGTAATATTTACAGGTTTGTTCCACTTATCGTAACATAAAATTAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAA                                                <  2:670966/149‑1                                                                                                        TTATAGGGAATATTTACAGGTTTGTTACACTTATCGTAACATAAAATTAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAA                                                    <  1:216920/144‑1                                                                                                            AGGGAATATTTACAGGTTTGTTACACTTATCGTAACATAAAATTAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAA                                                <  1:478500/144‑1                                                                                                              GGAATATTTACAGGTTTGTTACACTTATCGTAACATAAAATTAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCG                                         >  2:197114/1‑149                                                                                                              GGAATATTTACAGGTTTGTTACACTTATCGTAACATAAAATTAATTGACCATCAAGGCAAAATATCCCGCCCCGTCAGACGTATTATTTTTCATTAAAAATACTTAAGTATGCATTTCAATGCACCACGAAAGTGCAACAAACC                                              >  1:238592/1‑144                                                                                                                           GTTTGTTACACTTATCGTAACATAAAATTAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGA                            >  2:226501/1‑149                                                                                                                                TTACACTTATCGTAACATAAAATTAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATT                       <  2:84499/149‑1                                                                                                                                TTACACTTATCGTAACATAAAATTAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGA                            >  1:723966/1‑144                                                                                                                                 TACACTTATCGTAACATAAAATTAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAGTGCTTGAGTATACAGTTCAATGCACCACGATAGGGCAACAAACCTGGCGTGCACCATGATGATCATTC                      <  2:406121/149‑1                                                                                                                                  ACACTTATCGTAACATAAAATTAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATC                          <  1:530876/144‑1                                                                                                                                      TTATCGTAACATAAAATTAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCA                 >  2:329483/1‑149                                                                                                                                      TTATCGTAACATAAAATTAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCA                 >  2:775278/1‑149                                                                                                                                      TTATCGTAACATAAAATTAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCA                 >  2:607728/1‑149                                                                                                                                             aaCATAAAATTAATTGACCTTCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAG          <  2:920111/147‑1                                                                                                                                             aaCATAAAATTAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGG               <  1:45984/142‑1                                                                                                                                                ATAAAATTAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAACCCTGGCGTGCACCATGATGATCATTCCATCAGGTAC            >  1:98366/1‑144                                                                                                                                                   AAATTAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCC    >  2:892002/1‑149                                                                                                                                                    AATTAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCA   <  2:440333/149‑1                                                                                                                                                     ATTAATTGACCATCAAGGCAAAATCTCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAG  >  2:157110/1‑149                                                                                                                                                     ATTAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATCGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAG  >  2:294844/1‑149                                                                                                                                                     ATTAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAG  >  2:741419/1‑149                                                                                                                                                    ||||                                                                                                                                                   TTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  CP009273/2420188‑2420334 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑AATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAG  >  CP009273/2420335‑2420480

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.