Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	CP000730	62,818	+CAATTT	intergenic (+83/+228)	USA300HOU_0056 → / ← speG	hypothetical protein/diamine N‑acetyltransferase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	CP000730	= 62817	0 (0.000)	17 (0.880) +TCAATT	14/266	0.3	100%	intergenic (+82/+229)	USA300HOU_0056/speG	hypothetical protein/diamine N‑acetyltransferase
	?	CP000730	62818 =	0 (0.000)					intergenic (+83/+228)	USA300HOU_0056/speG	hypothetical protein/diamine N‑acetyltransferase

AATTATTAAAACAACGCAAACAAGGCTTACTACAAAAAATGTTTGTGTAATTCTGATAACGTCCTATTATGTTAATTGAAAATAGACAAAAATAAATTGAGAGCATCCCTTACCGCAAAGTGAAGGATGCTCTATTTTTGTTGAAATTAATATAAGCTTCATTCTGATAGATAGTCATTAATAATTGTTTGCAAATCGTTATTAAGTACCGTTGTTACAGCGTCATCATTCATAAAATCCTCGTAGATTTTATCAAGAATTTATTCCTCTTA  >  CP000730/62686‑62957                                                                                                                                    |                                                                                                                                             aaTTATTAAAACAACGCAAACAAGGCTTACTACAAAAAATGTTTGTGTAATTCTGATAACGTCCTATTATGTTAATTGAAAATAGACAAAAATAAATTGAGAGCATCCCTTACCGCAAAGTGAAGGATGCTCtcaatttat                                                                                                                                     <  2:222767‑M1/141‑10 (MQ=255)    tattaAAACAACGCAAACAAGGCTTACTACAAAAAATGTTTGTGTAATTCTGATAACGTCCTATTATGTTAATTGAAAATAGACAAAAATAAATTGAGAGCATCCCTTACCGCAAAGTGAAGGATGCTCtcaatttatttt                                                                                                                                  <  2:80329‑M1/141‑13 (MQ=255)               cGCAAACAAGGCTTACTACAAAAAATGTTTGTGTAATTCTGATAACGTCCTATTATGTTAATTGAAAATAGACAAAAATAAATTGAGAGCATCCCTTACCGCAAAGTGAAGGATGCTCtcaatttatttttgttgaaatta                                                                                                                       >  1:70684‑M1/1‑118 (MQ=255)                   aaCAAGGCTTACTACAAAAAATGTTTGTGTAATTCTGATAACGTCCTATTATGTTAATTGAAAATAGACAAAAATAAATTGAGAGCATCCCTTACCGCAAAGTGAAGGATGCTCtcaatttatttttgttgaaattaatat                                                                                                                   >  2:132026‑M1/1‑114 (MQ=255)                     cAAGGCTTACTACAAAAAATGTTTGTGTAATTCTGATAACGTCCTATTATGTTAATTGAAAATAGACAAAAATAAATTGAGAGCATCCCTTACCGCAAAGTGAAGGATGCTCtcaatttatttttgttgaaattaatataa                                                                                                                 <  1:165520‑M1/141‑30 (MQ=255)                                 ctaaaaatgTTTGTGTAATTCTGCGAACGTCCTATTATGTTAATTGAAAATAGACAAAAATAAATTGAGAGCATCCCTTACCGCAAAGTGAAGGATGCTCtcaatttatttttgttgaaattaatataagcttcattctga                                                                                                     <  1:105460‑M1/139‑42 (MQ=255)                                 cAAAAAATGTTTGTGTAATTCTGATAACGTCCTATTATGTTAATTGAAAATAGACAAAAATAAATTGAGAGCATCCCTTACCGCAAAGTGAAGGATGCTCtcaatttatttttgttgaaattaatataagcttcattctga                                                                                                     <  2:191785‑M1/141‑42 (MQ=255)                                 cAAAAAATGTTTGTGTAATTCTGATAACGTCCTATTATGTTAATTGAAAATAGACAAAAATAAATTGAGAGCATCCCTTACCGCAAAGTGAAGGATGCTCtcaatttatttttgttgaaattaatataagcttcattctga                                                                                                     <  2:120245‑M1/141‑42 (MQ=255)                                       atttttgtgTAATTCTGATAACGTCCTATTATGTTAATTGAAAATAGACAAAAATAAATTGAGAGCATCACTTACCGCAAAGTGAAGGATGCTCtcaatttatttttgttgaaattaatataagattcattctgatagata                                                                                               <  2:13001‑M1/138‑48 (MQ=255)                                       atggttcaataattcgaatAACGTCCTATTATGTTAATTGAAAATAGACAAAAATAAATTGAGAGCAGCCCTTACCGCAAAGTGAAGGATGCTCtcaatttatttttgttgaaattaatataagcttcattctgatagata                                                                                               <  2:8236‑M1/132‑48 (MQ=255)                                                       gATAACGTCCTATTATGTTAATTGAAAATAGACAAAAATAAATTGAGAGCATCCCTTACCGCAAAGTGAAGGATGCTCtcaatttatttttgttgaaattaatataagcttcattctgatagatagtcattaataattgtt                                                                               <  2:123951‑M1/141‑64 (MQ=255)                                                                                aaaTAGACAAAAATAAATTGAGAGCATCCCTTACCGCAAAGTGAAGGATGCTCtcaatttatttttgttgaaattaatataagcttcattctgatagatagtcattaataattgtttgcaaatcgttattaagtaccgttg                                                      <  2:12060‑M1/141‑89 (MQ=255)                                                                                               aatTGAGAGCATCCCTTACCGCAAAGTGAAGGATGCTCtcaatttatttttgttgaaattaatataagcttcattctgatagatagtcattaataattgtttgcaaatcgttattaagtaccgttgttacagcgtcatcat                                       >  2:83620‑M1/1‑38 (MQ=255)                                                                                                    agagCATCCCTTACCGCAAAGTGAAGGATGCTCtcaatttatttttgttgaaattaatataagcttcattctgatagatagtcattaataattgtttgcaaatcgttattaagtaccgttgttacagcgtcatcattcata                                  <  2:216085‑M1/141‑109 (MQ=255)                                                                                                    agagCATCCCTTACCGCAAAGTGAAGGATGCTCtcaatttatttttgttgaaattaatataagcttcattctgatagatagtcattaataattgtttgcaaatcgttattaagtaccgttgttacagcgtcatcattcata                                  <  2:102887‑M1/141‑109 (MQ=255)                                                                                                     gagCATCCCTTACCGCAAAGTGAAGGATGCTCtcaatttatttttgttgaaattaatataagcttcattctgatagatagtcattaataattgtttgcaaatcgttattaagtaccgttgttacagcgtcatcattcataa                                 <  1:18885‑M1/141‑110 (MQ=255)                                                                                                            ccTTACCGCAAAGTGAAGGATGCTCtcaatttatttttgttgaaattaatataagcttcattctgatagatagtcattaataattgtttgcaaatcgttattaagtaccgttgttacagcgtcatcattcataaaatcctc                          >  1:146292‑M1/1‑25 (MQ=255)                                                                                                                                    ctcaatttatttttgttgaaattaatataagcttcattctgatagatagtcattaataattgtttgcaaatcgttattaagtaccgttgttacagcgtcatcattcataaaatcctcgtagattttatcaagaatttattc  >  2:70918‑M1/1‑1 (MQ=255)                                                                                                                                    ctcaatttatttttgttgaaattaatataagcttcattctgatagatagtcattaataattgtttgcaaatcgttattaagtaccgttgttacagcgtcatcattcataaaatcctcgtagattttatcaagaatttattc  >  2:30514‑M1/1‑1 (MQ=255)                                                                                                                                    |                                                                                                                                             AATTATTAAAACAACGCAAACAAGGCTTACTACAAAAAATGTTTGTGTAATTCTGATAACGTCCTATTATGTTAATTGAAAATAGACAAAAATAAATTGAGAGCATCCCTTACCGCAAAGTGAAGGATGCTCTATTTTTGTTGAAATTAATATAAGCTTCATTCTGATAGATAGTCATTAATAATTGTTTGCAAATCGTTATTAAGTACCGTTGTTACAGCGTCATCATTCATAAAATCCTCGTAGATTTTATCAAGAATTTATTCCTCTTA  >  CP000730/62686‑62957

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.