Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	CP000730	62,818	+CAATTT	intergenic (+83/+228)	USA300HOU_0056 → / ← speG	hypothetical protein/diamine N‑acetyltransferase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	CP000730	= 62817	0 (0.000)	17 (0.880) +TCAATT	14/266	0.3	100%	intergenic (+82/+229)	USA300HOU_0056/speG	hypothetical protein/diamine N‑acetyltransferase
	?	CP000730	62818 =	0 (0.000)					intergenic (+83/+228)	USA300HOU_0056/speG	hypothetical protein/diamine N‑acetyltransferase

AAATAGAATTATTAAAACAACGCAAACAAGGCTTACTACAAAAAATGTTTGTGTAATTCTGATAACGTCCTATTATGTTAATTGAAAATAGACAAAAATAAATTGAGAGCATCCCTTACCGCAAAGTGAAGGATGCTCTATTTTTGTTGAAATTAATATAAGCTTCATTCTGATAGATAGTCATTAATAATTGTTTGCAAATCGTTATTAAGTACCGTTGTTACAGCGTCATCATTCATAAAATCCTCGTAGATTTTATCAAGAATTTATTC  >  CP000730/62680‑62951                                                                                                                                           |                                                                                                                                      aattattaaaacaacgcaaacaaggcttactacaaaaaatgtttgtgtaattctgataacgtcctattatgttaattgaaaatagacaaaaataaattgagagcatcccttaccgcaaagtgaaggatgctctcaatttat                                                                                                                                     <  2:222767‑M2/3‑1 (MQ=255)    tattaaaacaacgcaaacaaggcttactacaaaaaatgtttgtgtaattctgataacgtcctattatgttaattgaaaatagacaaaaataaattgagagcatcccttaccgcaaagtgaaggatgctctcaattTAtttt                                                                                                                                  <  2:80329‑M2/6‑1 (MQ=255)               cgcaaacaaggcttactacaaaaaatgtttgtgtaattctgataacgtcctattatgttaattgaaaatagacaaaaataaattgagagcatcccttaccgcaaagtgaaggatgctctcaattTATTTTTGTTGAAATTa                                                                                                                       >  1:70684‑M2/125‑141 (MQ=255)                   aacaaggcttactacaaaaaatgtttgtgtaattctgataacgtcctattatgttaattgaaaatagacaaaaataaattgagagcatcccttaccgcaaagtgaaggatgctctcaattTATTTTTGTTGAAATTAatat                                                                                                                   >  2:132026‑M2/121‑141 (MQ=255)                     caaggcttactacaaaaaatgtttgtgtaattctgataacgtcctattatgttaattgaaaatagacaaaaataaattgagagcatcccttaccgcaaagtgaaggatgctctcaattTATTTTTGTTGAAATTAATATaa                                                                                                                 <  1:165520‑M2/23‑1 (MQ=255)                                 ctaaaaatgtttgtgtaattctgcgaacgtcctattatgttaattgaaaatagacaaaaataaattgagagcatcccttaccgcaaagtgaaggatgctctcaattTATTTTTGTTGAAATTAATATAAGCTTCATTCTga                                                                                                     <  1:105460‑M2/35‑1 (MQ=255)                                 caaaaaatgtttgtgtaattctgataacgtcctattatgttaattgaaaatagacaaaaataaattgagagcatcccttaccgcaaagtgaaggatgctctcaattTATTTTTGTTGAAATTAATATAAGCTTCATTCTga                                                                                                     <  2:191785‑M2/35‑1 (MQ=255)                                 caaaaaatgtttgtgtaattctgataacgtcctattatgttaattgaaaatagacaaaaataaattgagagcatcccttaccgcaaagtgaaggatgctctcaattTATTTTTGTTGAAATTAATATAAGCTTCATTCTga                                                                                                     <  2:120245‑M2/35‑1 (MQ=255)                                       atttttgtgtaattctgataacgtcctattatgttaattgaaaatagacaaaaataaattgagagcatcacttaccgcaaagtgaaggatgctctcaattTATTTTTGTTGAAATTAATATAAGATTCATTCTgatagata                                                                                               <  2:13001‑M2/41‑1 (MQ=255)                                       atggttcaataattcgaataacgtcctattatgttaattgaaaatagacaaaaataaattgagagcagcccttaccgcaaagtgaaggatgctctcaattTATTTTTGTTGAAATTAATATAAGCTTCATTCTgatagata                                                                                               <  2:8236‑M2/41‑1 (MQ=255)                                                       gataacgtcctattatgttaattgaaaatagacaaaaataaattgagagcatcccttaccgcaaagtgaaggatgctctcaattTATTTTTGTTGAAATTAATATAAGCTTCATTCTGATAGATAGTCATTAATAATTGtt                                                                               <  2:123951‑M2/57‑1 (MQ=255)                                                                                aaatagacaaaaataaattgagagcatcccttaccgcaaagtgaaggatgctctcaattTATTTTTGTTGAAATTAATATAAGCTTCATTCTGATAGATAGTCATTAATAATTGTTTGCAAATCGTTATTAAGTACCgttg                                                      <  2:12060‑M2/82‑1 (MQ=255)                                                                                               aattgagagcatcccttaccgcaaagtgaaggatgctctcaattTATTTTTGTTGAAATTAATATAAGCTTCATTCTGATAGATAGTCATTAATAATTGTTTGCAAATCGTTATTAAGTACCGTTGTTACAGCGTcatcat                                       >  2:83620‑M2/45‑141 (MQ=255)                                                                                                    agagcatcccttaccgcaaagtgaaggatgctctcaattTATTTTTGTTGAAATTAATATAAGCTTCATTCTGATAGATAGTCATTAATAATTGTTTGCAAATCGTTATTAAGTACCGTTGTTACAGCGTCATCATTCATa                                  <  2:216085‑M2/102‑1 (MQ=255)                                                                                                    agagcatcccttaccgcaaagtgaaggatgctctcaattTATTTTTGTTGAAATTAATATAAGCTTCATTCTGATAGATAGTCATTAATAATTGTTTGCAAATCGTTATTAAGTACCGTTGTTACAGCGTCATCATTCATa                                  <  2:102887‑M2/102‑1 (MQ=255)                                                                                                     gagcatcccttaccgcaaagtgaaggatgctctcaattTATTTTTGTTGAAATTAATATAAGCTTCATTCTGATAGATAGTCATTAATAATTGTTTGCAAATCGTTATTAAGTACCGTTGTTACAGCGTCATCATTCATaa                                 <  1:18885‑M2/103‑1 (MQ=255)                                                                                                            ccttaccgcaaagtgaaggatgctctcaattTATTTTTGTTGAAATTAATATAAGCTTCATTCTGATAGATAGTCATTAATAATTGTTTGCAAATCGTTATTAAGTACCGTTGTTACAGCGTCATCATTCATAAAATCCTc                          >  1:146292‑M2/32‑141 (MQ=255)                                                                                                                                    ctcaattTATTTTTGTTGAAATTAATATAAGCTTCATTCTGATAGATAGTCATTAATAATTGTTTGCAAATCGTTATTAAGTACCGTTGTTACAGCGTCATCATTCATAAAATCCTCGTAGATTTTATCAAGAATTTATTc  >  2:70918‑M2/8‑141 (MQ=255)                                                                                                                                    ctcaattTATTTTTGTTGAAATTAATATAAGCTTCATTCTGATAGATAGTCATTAATAATTGTTTGCAAATCGTTATTAAGTACCGTTGTTACAGCGTCATCATTCATAAAATCCTCGTAGATTTTATCAAGAATTTATTc  >  2:30514‑M2/8‑141 (MQ=255)                                                                                                                                           |                                                                                                                                      AAATAGAATTATTAAAACAACGCAAACAAGGCTTACTACAAAAAATGTTTGTGTAATTCTGATAACGTCCTATTATGTTAATTGAAAATAGACAAAAATAAATTGAGAGCATCCCTTACCGCAAAGTGAAGGATGCTCTATTTTTGTTGAAATTAATATAAGCTTCATTCTGATAGATAGTCATTAATAATTGTTTGCAAATCGTTATTAAGTACCGTTGTTACAGCGTCATCATTCATAAAATCCTCGTAGATTTTATCAAGAATTTATTC  >  CP000730/62680‑62951

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.