| New junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | NC_000913_3_KO_pgi | 1190698 = | 38 (1.040) | 5 (0.140) | 5/270 | 4.3 | 11.7% | coding (1289/1371 nt) | purB | adenylosuccinate lyase |
| ? | NC_000913_3_KO_pgi | 1190713 = | 39 (1.120) | coding (1274/1371 nt) | purB | adenylosuccinate lyase | |||||
| Rejected: Coverage evenness skew score above cutoff. | |||||||||||
| Rejected: Frequency below/above cutoff threshold. | |||||||||||
GGAAGTGCTGGCTGAACCAATCCAGACAGTTATGCGTCGCTATGGCATCGAAAAACCGTACGAGAAGCTGAAAGAGCTGACTCGCGGTAAGCGCGTTGACGCCGAAGGCATGAAGCAGTTTATCGATGGTCTGGCGTTGCCAGA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ < NC_000913_3_KO_pgi/1190841‑1190698‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gccagaCCATCGATAAACTGCTTCATGCCTTCGGCGTCAACGCGCTTACCGCGAGTCAGCTCTTTCAGCTTCTCGTACGGTTTTTCGATGCCATAGCGACGCATAACTGTCTGGATTGGTTCAGCCAGCACTTCCCAGTTGTGATCCAGTT > NC_000913_3_KO_pgi/1190713‑1190857 GGAAGTGCTGGCTGAACCAATCCAGACAGTTATGCGTCGCTATGGCATCGAAAAACCGTACGAGAAGCTGAAAGAGCTGACTCGCGGTAAGCGCGTTGACGCCGAAGGCATGAAGCANTTTATCGATGGTCCGGCGCTGCCNGAgcggg > 2:165547/1‑144 CGATGGTCTGGCGTTGCCAGACCATCGATAAACTGCTTCATGCCTTCGGCGTCAACGCGCTTACCGC > 1:347490/1‑67 CGATGGTCTGGCGTTGCCAGACCATCGATAAACTGCTTCATGCCTTCGGCGTCAACGCGCTTACCGC < 2:347490/67‑1 ATGGTCTGGCGTTGCCAGACCATCGATAAACTGCTTCATGCCTTCGGCGTCAACGCGCTTACCGCGAGTCAGCTCTTTCAGCTTCTCGTACGGTTTTTCGATGCCATAGCGACGCATAACTGTCTGGATTGGTTCAGCCAGCACTTCCC > 1:114774/1‑149 TGGTCTGGCGTTGCCAGACCATCGATAAACTGCTTCATGCCTTCGGCGTCAACGCGCTTACCGCGAGTCAGCTCTTTCAGCTTCTCGTACGGTTTTTCGATGCCATAGCGAC > 1:324466/1‑112 TGGTCTGGCGTTGCCAGACCATCGATAAACTGCTTCATGCCTTCGGCGTCAACGCGCTTACCGCGAGTCAGCTCTTTCAGCTTCTCGTACGGTTTTTCGATGCCATAGCGAC < 2:324466/112‑1 GCCAGACCATCGATAAACTGCTTCATGCCTTCGGCGTCAACGCGCTTACCGCGAGTCAGCTCTTTCAGCTTCTCGTACGGTTTTTCGATGCCATAGCGACGCATAACTGTCTGGATTGGTTCAGCCAGCACTTCCCAGTTGTGATCCAG < 2:209085/149‑1 CAGACCATCGATAAACTGCTTCATGCCTTCGGCGTCAACGCGCTTACCGCGAGTCAGCTCTTTCAGCTTCTCGTACGGTTTTTCGATGCCATAGCGACGCATAACTGTCTGGATTGGTTCAGCCAGCACTTCCCAGTTGTGATCCAGTT > 1:585872/1‑149 GGAAGTGCTGGCTGAACCAATCCAGACAGTTATGCGTCGCTATGGCATCGAAAAACCGTACGAGAAGCTGAAAGAGCTGACTCGCGGTAAGCGCGTTGACGCCGAAGGCATGAAGCAGTTTATCGATGGTCTGGCGTTGCCAGA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ < NC_000913_3_KO_pgi/1190841‑1190698‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gccagaCCATCGATAAACTGCTTCATGCCTTCGGCGTCAACGCGCTTACCGCGAGTCAGCTCTTTCAGCTTCTCGTACGGTTTTTCGATGCCATAGCGACGCATAACTGTCTGGATTGGTTCAGCCAGCACTTCCCAGTTGTGATCCAGTT > NC_000913_3_KO_pgi/1190713‑1190857 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |