New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_KO_pgi	1190698 =	38 (1.040)	5 (0.140)	5/270	4.3	11.7%	coding (1289/1371 nt)	purB	adenylosuccinate lyase
	?	NC_000913_3_KO_pgi	1190713 =	39 (1.120)					coding (1274/1371 nt)	purB	adenylosuccinate lyase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GATAATCTTAATAAGCAGGCCGGACAGCATCGCCATCCGGCACTGATACGAGGTTTATTTCAGCTCATCAACCATCGTGATAGCTCGACCAATATAGTTAGCCGGCGTCATCGCTTTCAGGCGGGCTTTCTCTTCTTCTGGCAACGCCAGACCATCGATAAACTGCTTCATGCCTTCGGCGTCAACGCGCTTACCGCGAGTCAGCTCTTTCAGCTTCTCGTACGGTTTTTCGATGCCATAGCGACGCATAACTGTCTGGATTGGTTCAGCCAGCACTTCC  >  NC_000913_3_KO_pgi/1190562‑1190841                                                                                                                                         |                                                                                                                                                gATAATCTTAATAAGCAGGCCGGACAGCATCGCCATCCGGCACTGATACGAGGTTTATTTCAGCTCATCAACCATCGTGATAGCTCGACCAATATAGTTAGCCGGCGTCATCGCTTTCAGGCGGGCTTTCTCTTCTTCTGGCAACGCCa                                                                                                                                     <  1:190007/149‑1 (MQ=255)      tCTTAATAAGCAGGCCGGACAGCATCGCCATCCGGCACTGATACGAGGTTTATTTCAGCTCATCAACCATCGTGATAGCTCGACCAATATAGTTAGCCGGCGTCATCGCTTTCAGGCGGGCTTTCTCTTCTTCTGGCAACGCCAGACCa                                                                                                                                >  1:311394/1‑149 (MQ=255)           ataaGGAGGCCGGACAGCATCGCCATCCGGCACGGATACGATGTTTATTTCAGCTCATCAACCATCGTGATAGCTCGACCAATATAGTTAGCCGGCGTCATCGCTTTCAGGCGGGCTTTCTCTTCTTCTGGCAACGCCAGACCATCGAt                                                                                                                           <  2:73318/149‑1 (MQ=255)             aaGCAGGCCGGACAGCATCGCCATCCGGCACTGATACGAGGTTTATTTCAGCTCATCAACCATCGTGATAGCTCGACCAATATAGTTAGCCGGCGTCATCGCTTTCAGGCGGGCTTTCTCTTCTTCTGGCAACGCCAGACCATCGATaa                                                                                                                         >  1:306374/1‑149 (MQ=255)                      ggACAGAATCGCCATACGGCACGGATACGAGGGTTATTTCAGCTCATCAACCATCGTGATAGCTCGACCAATATAGTTAGCCGGTGTCATCGCTTTCAGGCGGGCTTTCTCTTCTTATGGCAACGCCAGACCATCGATAAACTGCTTCa                                                                                                                <  2:391097/149‑1 (MQ=255)                      cgACAGCATCGCCATCCGGCACTGATACGAAGTTTATTTCAGCTCATCAACCATCGTGATAGCTCGCCCAATATAGTTAGCCGGCGTCATCGCTTTCAGGCGGGCTTTCTCTTCTTCTGGCAACGCCAGACCATCGATAAACTGCTTCa                                                                                                                <  1:250745/148‑1 (MQ=255)                                     ccGGCACTGATACGAGGTTTATTTCAGCTCATCAACCATCTTGATAGCTCGACCAATATAGTTAGCCGGCGTCATCGCTTTCAGGCGGGCTTTCTCTTCTTCTGGCAACGCCAGACCATCGATAAACTGCTTCATGCCTTCGGCGTCaa                                                                                                 >  1:508084/1‑149 (MQ=255)                                        gCACTGATACGAGGTTTATTTCAGCTCATCAACCATCGTGATAGCTCGACCAATATAGTTAGCCGGCGTCATCGCTTTCAGGCGGGCTTTCTCTTCTTCTGGCAACGCCAGACCATCGATAAACTGCTTCATGCCTTCGGCGTCACcgc                                                                                              >  1:130416/1‑149 (MQ=255)                                          agtGATACGAGGTTTATTGCAGCTCATCAACCATCGTGATAGCTCGACCAATATAGTTAGCCGGCGTCATCGCTTTCAGGCGGGCTTTCTCTTCTTCTGGCAACGCCAGACCATCGATAAACTGCTTCATGCCTTCGGCGTCAACgcgc                                                                                            <  2:285390/147‑1 (MQ=255)                                              aTACGAGGTTTATTTCAGCTCATCAACCATCGTGATATCTCGACCAATATAGTTAGCCGGCGTCATCGCTTTCAGGCGGGCTTTCTCTTCTTCTGGCAACGCCAGACCATCGATAAACTGCTTCATGCCTTCGGCGTCAACGCGCTTAc                                                                                        >  2:619261/1‑149 (MQ=255)                                                  gAGGTTTATTTCAGCTCATCAACCATCGTGATAGCTCGACCAATATAGTTAGCCGGCGTCATCGCTTTCAGGCGGGCTTTCTCTTCTTCTGGCAACGCCAGACCATCGATAAACTGCTTCATGCCTTCGGCGTCAACGCGCTTACcgcg                                                                                    >  2:374483/1‑149 (MQ=255)                                                   atgtctttttCAGCTCATCAACCATCGTGATAGCTCGACCAATATAGTTAGCCGGCGTCATCGCTTTCAGGCGGGCTTTCTCTTCTTCTGGCAACGCCAGACCATCGATAAACTGCTTCATGCCTTCGGCGTCAACGCGCTTACCGCGa                                                                                   <  2:468343/142‑1 (MQ=255)                                                     gTTTATTTCAGCTCATCAACCATCGTGATAGCTCGACCAATATAGTTATCCGGCGTCATCGCTTTCAGGCGGGCTTTCTCTTCTTCTGGCAACGCCAGACCATCGATAAACTGCTTCATGCCTTCGGCGTCAACGCGCTTACCGCGAGt                                                                                 <  2:231408/149‑1 (MQ=255)                                                     gTTTATTTCAGCTCATCAACCATCGTGATAGCTCGACCAATATAGTTAGCCGGCGTCATCGCTTTCAGGCGGGCTTTCTCTTCTTCTGGCAACGCCAGACCATCGATAAACTGCTTCATGCCTTCGGCGTCAACGCGCTTCCCGCGact                                                                                 >  2:253275/1‑147 (MQ=255)                                                     gTTTATTTCAGCTCATCAACCATCGTGATAGCTCGACCAATATAGTTAGCCGGCGTCATCGCTTTCAGGCGGGCTTTCTCTTCTTCTGGCAACGCCAGACCATCGATAAACTGCTTCATGCCTTCGAAGTAAAAGCGCTTCCCGCGAgc                                                                                 >  2:421789/1‑148 (MQ=255)                                                              aGCTCATCAACCATGGTGATAGCTCGACCAATATAGTTAGCCGGCGTCATCGCTTTCAGGCGGGCTTTCTCTTCTTCTGTCAACGCCAGACCATCGATAAACTGCTTCATGCCTTCGGCGTCAACGCGCTTACCGCGAGTCAGCTCttt                                                                        <  2:524584/149‑1 (MQ=255)                                                                cTCATCAACCATCGTGATAGCTCGACCAATATAGTTAGCCGGCGTCATCGCTTTCAGGCGGGCTTTCTCTTCTTCTGGCAACGCCAGACCATCGATAAACTGCTTCATGCCTTCGGCGTCAGCGCGCTTACCGCGAGTCAGCTCTTTCa                                                                      <  2:130416/149‑1 (MQ=255)                                                                 tcatcaACCATCGTGATAGCTCGACCAATATAGTTAGCCGGCGTCATCGCTTTCAGGCGGGCTTTCTCTTCTTCTGGCAACGCCAGACCATCGATAATCTGCTTCATGCCTTCGGCGTCAACGCGCTTACCGCGAGTCAGCTCTTTCAg                                                                     <  1:409136/149‑1 (MQ=255)                                                                 tcatcaACCATCGTGATAGCTCGACCAATATAGTTAGCCGGCGTCATCGCTTTCAGGCGGGCTTTCTCTTCTTCTGGCAACGCCAGACCATCGATAAACTGCTTCATGCCTTCGGCGTCAACGCGCTTACCGCGAGTCAGCTCTTTccg                                                                     >  1:201222/1‑147 (MQ=255)                                                                    tcaACAATCGTGATAACTCGACCAATATAGTTAGCCGGCGTCATCGCTTTCAGGCGGGCTTGCTCTTCTTCTGGCAACGCCAGACCATCGATAAACTGCTTCATGCCTTCGGCGTCAACGCGCTTACCGCGAGTCAGCTCTTTCAGCtt                                                                  >  2:475147/1‑149 (MQ=255)                                                                      aaCCATCGTGATAGCTCGACCAATATAGTTAGCCGGCGTCATCGCTTTCAGGCGGGCTTTCTCTTCTTCTGGCAACGCCAGACCATCGATAAACTGCTTCATGCCTTCGGCGTCAACGCGCTTACCGCGAGTCAGCTCTTTCAGCTtct                                                                >  1:273986/1‑149 (MQ=255)                                                                        ccATCGTGATAGCTCGACCAATATAGTTAGCCGGCGTCATCGCTTTCAGGCGGACTTTCTCTTCTTCTGGCAACGCCAGACCATCGATAAACTGCTTCATGCCTTCGGCGTCAACGCCCTTACCGCGAGTCAGCTCTTTCAGCTTCCCg                                                              >  2:590209/1‑149 (MQ=255)                                                                         cATCGTGATAGCTCGACCAATATAGTTAGCCGGCGTCATCGATTTCAGGCGGGCTTTCTCTTCTTCTGGCAACGACAGACCATCGATAAACTGCTTCATGCCTTCGGCGTCAACGCGATTACCGCGACTCAGCTCTTTCAGCTTCTCgg                                                             >  1:513211/1‑148 (MQ=255)                                                                                   gCTCGCCCAATATCGTTAGCCGGCGTCATCGCTTTCAGGCGGGCTTTCTCTTCTTCTGGCAACGCCAGACCATCGATAAACTGCTTCATGCCTTCGTCGTCAACGCGCTTACCGCGAGTCGGCGCTTTCAGCTTCTCGTACGGTTTTTc                                                   >  2:41167/1‑149 (MQ=255)                                                                                       gACCAATATAGTTAGCCGGCGTCATCGCTTTCAGGCGGGCTTTCTCTTCTTCTGGCAACGCCAGACCATCGATAAACTGCTTCATGCCTTCGGCGTCAACGCGCTTACCGCGAGTCAGCTCTTTCAGCTTCTCGTACGGtt                                                       <  2:500717/141‑1 (MQ=255)                                                                                       gACCAATATAGTTAGCCGGCGTCATCGCTTTCAGGCGGGCTTTCTCTTCTTCTGGCAACGCCAGACCATCGATAAACTGCTTCATGCCTTCGGCGTCAACGCGCTTACCGCGAGTCAGCTCTTTCAGCTTCTCGTACGGtt                                                       >  1:500717/1‑141 (MQ=255)                                                                                                  ttAGCCGGCGTCATCGCTTTCAGGCGGGCTTTCTCTTCTTCTGGCAACGCCAGACCATCGATAAACTGCTTCATGCCTTCGGCGTCAACGCGCTTACCGCGAGTCAGCTCTTTCAGCTTCTCGTACGGTTTTTCGATGCCATAGCGACg                                    >  1:209085/1‑149 (MQ=255)                                                                                                         gCGTCATCGCTTTCAGGCGGGCTTTCTCTTCTTCTGGCAACGCCAGACCATCGATAACCTGCTTCATGCCTTCGGCGTCAACGCGCTTACCGCGAGTCAGCTCTTTCAGCTTCTCGTACGGTTTTTCGATGCCATAGCGACGCATAACt                             <  1:636061/149‑1 (MQ=255)                                                                                                         gCGTCATCGCTTTCAGGCGGGCTTTCTCTTCTTCTGGCAACGCCAGACCATCGATAAACTGCTTCATGCCTTCGGCGTCAACGCGCTTACCGCGAGTCAGCTCTTTCAGCTTCTCGTACGGTTTTTCGATGCCATAGCGACGCATAACt                             >  1:142917/1‑149 (MQ=255)                                                                                                              aTCGCTTTCAGGCGGGCTTTCTCTTCTTCTGGCAACGCCAGACCATCGATAAACTGCTTCATGCCTTCGGCGTCAACGCGCTTACCGCGAGTCAGCTCTTTCAGCTTCTCGTACGGTTTTTCGATGCCATAGCGACGCATAACTGTCTg                        <  2:508084/149‑1 (MQ=255)                                                                                                               tCGCTTTCAGGCGGGCTTTCTCTTCTTCTGGCAACGCCAGACCATCGATAAACTGCTTCATGCCTTCGGCGTCAACGCGCTTACCGCGAGTCAGCTCTTTCAGCTTCTCGTACGGTTTTTCGATGCCATAGCGACGCATAACTGTCTgg                       >  1:134140/1‑149 (MQ=255)                                                                                                               tCGCTTTCAGGCGGGCTTTCTCTTCTTCTGGCAACGCCAGACCATCGATAAACTGCTTCATGCCTTCGGCGTCAACGCGCTTACCGCGAGTCAGCTCTTTCAGCTTCTCGTACGGTTTTTCGATGCCATAGCGACGCATAACTGTCTgg                       >  2:293199/1‑149 (MQ=255)                                                                                                                    ttCAGGCGGGCTTTTTCTTCTTCTGGCAACGCCAGACCATCGATAAACTGCTTCATGCCTTCGGCGTCAACGCGCTTACCGCGAGTCAGCTCTTTCAGCTTCTCGTACGGTTTTTCGATGCCATAGCGACGCATAACTGTCTGGATTgg                  <  1:167939/149‑1 (MQ=255)                                                                                                                               tttCTCTTCTTCTGGCAACGCCAGACCATCGATAAACTGCTTCATGCCTTCGGCGTCAACGCGCTTACCGCGAGTCAGCTCTTTCAGCTTCTCGTACGGTTTTTCGATCCCGGGTCTACGCATAACTGTCTGGATTGGTTCAGCCAGCa       <  2:273986/149‑1 (MQ=255)                                                                                                                                  ctctTCTTCTGGCAACGCCAGACCATCGATAAACTGCTTCATGCCTTCGGCGTCAACGCGCTTACCGCGAGTCCGCTCTTTCAGCTTCTCGTACGGTTTTTCGATGCCATAGCCACGCATAACTGTCTAGATTGGTTCCGCCAGCACta    >  2:569479/1‑148 (MQ=255)                                                                                                                                   tcttcttctGGCAACGCCAGACCATCGATAAACTGCTTCATGCCTTCGGCGTCAACGCGCTTACCGCGAGTCAGCTCTTTCAGCTTCTCGTACGGTTTTTCGATGCCATAGCGACGCATAACTGTCTGGATTGGTTCAGCCAGCACTTc   >  2:430797/1‑149 (MQ=255)                                                                                                                                    cccgctcnGGCAGCGCCGGACCATCGATAAANTGCTTCATGCCTTCGGCGTCAACGCGCTTACCGCGAGTCAGCTCTTTCAGCTTCTCGTACGGTTTTTCGATGCCATAGCGACGCATAACTGTCTGGATTGGTTCAGCCAGCACTTcc  <  2:165547/144‑1 (MQ=255)                                                                                                                                        ttctGGCAACGCCAGACCATCGATAAACTGCTTCATGCCTTCGGCGTCAACGCGCTTACCGCGAGTCAGCTCTTTCAGCTTCTCGTACGGTTTTTCGATGCCATAGCGa                                      >  1:353168/1‑109 (MQ=255)                                                                                                                                        ttctGGCAACGCCAGACCATCGATAAACTGCTTCATGCCTTCGGCGTCAACGCGCTTACCGCGAGTCAGCTCTTTCAGCTTCTCGTACGGTTTTTCGATGCCATAGCGa                                      <  2:353168/109‑1 (MQ=255)                                                                                                                                         |                                                                                                                                                GATAATCTTAATAAGCAGGCCGGACAGCATCGCCATCCGGCACTGATACGAGGTTTATTTCAGCTCATCAACCATCGTGATAGCTCGACCAATATAGTTAGCCGGCGTCATCGCTTTCAGGCGGGCTTTCTCTTCTTCTGGCAACGCCAGACCATCGATAAACTGCTTCATGCCTTCGGCGTCAACGCGCTTACCGCGAGTCAGCTCTTTCAGCTTCTCGTACGGTTTTTCGATGCCATAGCGACGCATAACTGTCTGGATTGGTTCAGCCAGCACTTCC  >  NC_000913_3_KO_pgi/1190562‑1190841

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.