New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_KO_pgi	= 4404097	45 (1.240)	5 (0.140)	5/276	4.3	10.1%	coding (977/1299 nt)	purA	adenylosuccinate synthetase
	?	NC_000913_3_KO_pgi	= 4404109	45 (1.260)					coding (989/1299 nt)	purA	adenylosuccinate synthetase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CGACCGAACTGTTTGATGAAACTGGCGAGTTCCTCTGCAAGCAGGGTAACGAATTCGGCGCAACTACGGGGCGTCGTCGTCGTACCGGCTGGCTGGACACCGTTGCCGTTCGTCGTGCGGTACAGCTGAACTCCCTGTCTGGCTTCTGCCTGACTAAACTGGACGTTCTGGATGGCCTGAAAGAGGTTAAACTCTGCGTGGCTTACCGTATGCCGGATGGTCGCGAAGTGACTACCACTCCGCTGGCAGCTGACGACTGGAAAGGTGTAGAGCCGATTTACGAAACC  >  NC_000913_3_KO_pgi/4403959‑4404245                                                                                                                                           |                                                                                                                                                     cgaccgaACTGTTTGATGAAACTGGCGAGTTCCTCTGCAAGCAGGGTAACGAATTCGGCGCAACTACGGGGCGTCGTCGTCGTACCGGCTGGCTGGACACCGTTGCCGTTCGTCGTGCGGTACAGCTGAACTCCCTGTCTGGCTTCTGc                                                                                                                                            <  1:307071/149‑1 (MQ=255)  gaccgaACTGTTTGATGAAACTGGCGAGTTCCTCTGCAAGCAGGGTAACGAATTCGGCGCAACTACGGGGCGTCGTCGACGTACCGGCTGGCTGGATACCGTTGCCGTTCGACGTGCGGTACAGCTGAACTCCCTGTCTGGCTTCTGcc                                                                                                                                           >  1:589111/1‑149 (MQ=255)   accgaACTGTTTGATGAAACTGGCGAGTTCCTCTGCAAGCAGGGTAACGAATTCGGCGCAACTACGGGGCGTCGTCGTCGTACCGGCTGGCTGGACACCGTTGCCGTTCGTCTTGCGGTACAGCTGAACTCCCTGTCTGGCTTCTGCCt                                                                                                                                          <  2:459666/149‑1 (MQ=255)   accgaACTGTTTGATGAAACTGGCGAGTTCCTCTGCAAGCAGGGTAACGAATTCGGCGCAACTACGGGGCGTCGTCGTCGTACCGGCTGGCTGGACACCGTTGCCGTTCGTCGTGCGGTACAGCTGAACTCCCTGTCTGGCTTCTGCCt                                                                                                                                          >  1:262575/1‑149 (MQ=255)   accgaACTGTTTGATGAAACTGGCGAGTTCCTCTGCAAGCAGGGTAACGAATTCGGCGCAACTACGGGGCGTCGTCGTCGTACCGGCTGGCTGGACACCGTTGCCGTTCGTCGTGCGGTACAGCTGAACTCCCTGTCTGGCTTCTGCCt                                                                                                                                          >  2:557451/1‑149 (MQ=255)     cgaACTGTTTGACGAAACGGGCGAGTTCCTCTGCAAGCAGGGTAACGAATTCGGCGCAACTACGGGGCGTCGTCGTCGTACCGGCTGGCTGGACACCGTTGCCGTTCGTCGTGCGGTACAGCTGAACTCCCTGTCTGGCTTCTGCCTGa                                                                                                                                        <  1:247667/149‑1 (MQ=255)       aaCTGTTTGATGAAACTGGCGAGTTCCTCTTCAAGCAGGGTAACGAATTCGGCGCAACTACGGGGCGTCGTCGTCGTACCGGCTGGCTGGACACCGTTGCCGTTCGTCGTGCGGTACAGCTGAACTCCCTGTCTGGCTTCTGCCTGACt                                                                                                                                      >  2:461756/1‑149 (MQ=255)          tGTTTGATGAAACTGGCGAGTTCCTCTGCAAGCAGGGTAACGAATTCGGCGCAACTACGGGGCGTCGTCGTCGTACCGGCTGGCTGGACACCGTTGCCGTTCGTCGTGCGGTACAGCTGAACTCCCTGTCTGGCTTCTGCCTGACTaaa                                                                                                                                   >  2:111730/1‑149 (MQ=255)          tGTTTGATGAAACTGGCGAGTTCCTCTGCAAGCAGGGTAACGAATTCGGCGCAACTACGGGGCGTCGTCGTCGTACCGGCTGGCTGGACACCGTTGCCGTTCGTCGTGCGGTACAGCTGAACTCCCTGTCTGGCTTCTGCCTGACTaaa                                                                                                                                   <  1:557451/149‑1 (MQ=255)           gTTTGATGAAACTGGCGAGTTCCTCTGCAAGCAGGGTAACGAATTCGGCGCAACTACGGGGCGTCGTCGTCGTACCGGCTGGCTGGACACCGTTGCCGTTCGTCGTGCGGTACAGCTGAACTCCCTGTCTGGCTTCTGCCTGACTAAAc                                                                                                                                  <  1:312300/149‑1 (MQ=255)           gTTTGATGAAACTGGCGAGTTCCTCTGCAAGCAGGGTAACGAATTCGGCGCAACTACGGGGCGTCGTCGTCGTACCGGCTGGCTGGACACCGTTGCCGTTCGTCGTGCGGTACAGCTGAACTCCCTGTCTGGCTTCTGCCTGACTAAAc                                                                                                                                  >  2:574064/1‑149 (MQ=255)                     aCTGGCGAGTTTCTCTGCAAGCAGGGTAACGAATTCGGCGCAACTACGGGGCGTCGTCGGCGTACCGGCTGGCTGGACACCGTTGCCGTTCGTCGTGCGGTACAGCTGAACTCCCTGTCTGGCTTTTGCCTGACTAATCTGGACGTTCt                                                                                                                        <  2:588786/149‑1 (MQ=255)                       tGGCGAGTTCCTCTGCAAGCAGGGTAACGAATTCGGCGCCATTCAAGTCCTTCGTCGTCGTACCGGCTGGCTGGACACCGTTGCCGTTCGTCGTGCGGTACAGCTGAACTCCCTGTCTGGCTTCTGCCTGACTAAACTGGACGTTCTgg                                                                                                                      <  1:212711/149‑1 (MQ=255)                         gCGAGTTCCTCTGCAAGCAGGGTAACGAATTCGGCGCAACTACGGGGCGTCGTCGTCGTACCGGCTGGCTGGACACCGTTGCCGTTCGTCGTGCGGTACAGCTGAACTCCCTGTCTGGCTTCTGCCTGACTAAACTGGACGTTCTGGAt                                                                                                                    >  2:536231/1‑149 (MQ=255)                              ttCCTCTGCAAGCAGGGTAACGAATTCGGCGCAACTACGGGGCGTCGTCGTCGTACCGGCTGGCTGGACACCGTTGCCGTTCGTCGTGCGGTACAGCTGAACTCCCTGTCTGGCTTCTGCCTGACTAACCTGGCCGTTCTGGATGGCCt                                                                                                               >  1:59334/1‑149 (MQ=255)                              ttCCTCTGCAAGCAGGGTAACGAATTCGGCGCAACTACGGGGCGTCGTCGTCGTACCGGCTGGCTGGACACCGTTGCCGTTCGTCGTGCGGTACAGCTGAACTCCCTGTCTGGCTTCTGCCTGACTAAACTGGACGTTCTGGATGGCCt                                                                                                               <  2:129744/149‑1 (MQ=255)                                     gCAAGCAGGGTAACGAATTCGGCGCAACTACGGGGCGTCGTCGTCGTACCGGCTGGCTGGACACCGTTGCCGTTCGTCGTGCGGTACAGCTGAACTCCCTGTCTGGCTTCTGCCTGACTaaa                                                                                                                                   <  2:595124/122‑1 (MQ=255)                                     gCAAGCAGGGTAACGAATTCGGCGCAACTACGGGGCGTCGTCGTCGTACCGGCTGGCTGGACACCGTTGCCGTTCGTCGTGCGGTACAGCTGAACTCCCTGTCTGGCTTCTGCCTGACTaaa                                                                                                                                   >  1:595124/1‑122 (MQ=255)                                     gCAAGCAGGGTAACGAATTCGGCGCAACTACGGGGCGTCGTCGTCGTACCGGCTGGCTGGACACCGTTGCCGTTCGTCGTGCGGTACAGCTGAACTCCCTGTCTGGCTTCTGCCTGACTAAACTGGACGTTCTGGATGGCCTGAAagag                                                                                                        <  1:111730/149‑1 (MQ=255)                                     gCAAGCAGGGTAACGAATTCGGCGCAACTACGGGGCGTCGTCGTCGTACCGGCTGGCTGGACACCGTTGCCGTTCGTCGTGCGGTACAGCTGAACTCCCTGTCTGGCTTCTGCCTGACTAAACTGGACGTTCTGGATGGCCTGAAagag                                                                                                        <  1:32012/149‑1 (MQ=255)                                        aGCATGGTAACGAACTTGGCGCAACCACGGGGCGTCGTCGTCGTACCGGCTGGCTGGACACCGTTGCCGTTCGTCGTGCGGTACAGCTGAACTCCCTGTCTGGCTTCTGCCTGACTAAACTGGACGTTCTGGATGGCCTGAAAGAGGtt                                                                                                     <  2:346765/149‑1 (MQ=255)                                                  cGAATTCGGCGCAACTACGGGGCGTCGTCGTCGTACCGGCTGGCTGGACACCGTTGCCGTTCGTCGTGCGGTACAGCTGAACTCCCTGTCTGGCTTCTGCCTGACTAAACTGGACGTTCTGGATGGCCTGAAAGAGGTTAAACTCTGCg                                                                                           >  1:521182/1‑149 (MQ=255)                                                               aCTACGGGGCGTCGTCGTCGTACCGGCTGGCTGGACACCGTTGCCGTTCGTCGTGCGGTACAGCTGAACTCCCTGTCTGGCTTCTGCCTGACTAAACTGGACGTTCTGGATGGCCTGAAAGAGGTTAAACTCTGCGTGGCTTACCGTAt                                                                              <  1:536231/149‑1 (MQ=255)                                                                 tACGGGGCGTCGTCGTCGTACCGGCTGGCTGGACACCGTTGCCGTTCGTCGTGCGGTACAGCTGAACTCCCTGTCTGGCTTCTGCCTGACTAAACTGGACGTTCt                                                                                                                        <  1:200999/105‑1 (MQ=255)                                                                 tACGGGGCGTCGTCGTCGTACCGGCTGGCTGGACACCGTTGCCGTTCGTCGTGCGGTACAGCTGAACTCCCTGTCTGGCTTCTGCCTGACTAAACTGGACGTTCt                                                                                                                        >  2:200999/1‑105 (MQ=255)                                                                                 cgtACCGGCTGGCTGGACACCGTTGCCGTTCGTCGTGCGGTACAGCTGAACTCCCTGTCTGGCTTCTGCCTGACTAAACTGGACGTTCTGGATGGCCTGAAAGAGGTTAAACTCTGCGTGGCTTACCGTATGCCGGATGGTCGCGAAGt                                                            <  2:262575/149‑1 (MQ=255)                                                                                 cgtACCGGCTGGCTGGACACCGTTGCCGTTCGTCGTGCGGTACAGCTGAACTCCCTGTCTGGCTTCTGCCTGACTAAACTGGACGTTCTGGATGGCCTGAAAGAGGTTAAACTCTGCGTGGCTTACCGTATGCCGGATGGTCGCGAAGt                                                            <  2:614381/149‑1 (MQ=255)                                                                                     ccGGCTGGCTGGACACCGTTGCCGTTCGTCGTGCGGTACAGCTGAACTCCCTGTCTGGCTTCTGCCTGACTAAACTGGACGTTCTGGATGGCCTGAAAGAGGTTAAACTCTGCGTGGCTTACCGTATGCCGGATGGTCGCGACGTGACt                                                        >  2:595699/1‑149 (MQ=255)                                                                                      cGGCTGGCTGGACCCCGTCGCCGTTCGTCGTGCTGTACAGCTGAACTCCCTGGCTGGCTTCTGCCTGACTAAACTGGACGTTCTGGATGGCCTGAAAGAGGTTAAACTCTGCGTGGCTTACCGTATGCCGGATGGTCGCGAAGTGACTa                                                       <  2:290184/149‑1 (MQ=255)                                                                                                 gcacCGGTGCCGTTCGTCGTGCGGTACAGCTGATCTCCCTGTCTGGCTTCTGCCTGACTAAACTGGACGTTCTGGATGGCCTGAACGAGGTTAAACTCTGCGTGGCTTACCGTATGCCGGATGGTCGCGAAGTGACTACCACTCCGCTg                                            <  2:567533/148‑1 (MQ=255)                                                                                                    ccgtgcccgtccgtCGTGCTGTACAGCTGAACTCCGTGTCGGGCTTCTGCCTGACTAAACTGGACGTTCTGGATGGCCTGAAATAGGTTAAACGCTGCGTGGCTTACCGTATGCCGGATGGTCGCGAAGTGACTACCACTCCGCTGGCa                                         <  2:528875/143‑1 (MQ=255)                                                                                                         gCCGTTCGTCGTGCGGTACAGCTGAACTCCCTGTCTGGCTTCTGCCTGACTAAACTGGACGTTCTGGATGGCCTGAAAGAGGTTAAACTCTGCGTGGCTTACCGTATGCCGGATGGTCGCGAAGTGACTACCACTCCGCTGCCAGCtaa                                    >  2:46040/1‑147 (MQ=255)                                                                                                           cGTTCGTCGTGCGGTACAGCTGAACTCCCTGTCTGGCTTCTGCCTGACTAAACTGGACGTTCTGGATGGCCTGAAAGAGGTTAAACTCTGCGTGGCTTACCGTCTGCCGGATGGTCGCGAAGTGACTACCACTCCGCTGGCAGCTgacc                                  >  2:158179/1‑148 (MQ=255)                                                                                                            gTTCGGCGTGCGGGACAGCTGAACTCCCTGTCTGGCTTCTGCCTGACTAAACTGGACGTTCTGGATGGCCTGAAAGAGGTTAAACTCTGCGTGGCTTACCGTATGCCGGATGGTCGCGAAGTGACTACCACTCCGCTGGCAGCTgacga                                 <  2:55676/149‑1 (MQ=255)                                                                                                             ttCGTCGTGCGGTACAGCTGAACTCCCTGTCTGGCTTCTGCCTGACTAAACTGGACGTTCTGGATGGCCTGAAAGAGGTTAAACTCTGCGTGGCTTACCGTATGCCGGATGGTCGCGAAGTGACTACCACTCCGCTGGCAGCTgacgac                                >  1:349916/1‑149 (MQ=255)                                                                                                                 tcgtGCGGTACAGCTGAACTCCCTGTCTGGCTTCTGCCTGACTAAACTGGACGTTCTGGATGGCCTGAAAGAGGTTAAACTCTGCGTGGCTTACCGTATGCCGGATGGTCGCGAAGTGACTACAAACACGCTGGCAGCTGACGACGGGa                            >  1:280427/1‑149 (MQ=255)                                                                                                                   gtGCGGTACAGCTGAACTCCCTGTCTGGCTTCTGCCTGACTAAACTGGACGTTCTGGATGGCCTGAAAGAGGTTAAACTCTGCGTGGCTTACCGTATGCCGGATGGTCGCGAAGTGACTACCACTCCGCTGGCAGCTGACGACTGGaaa                          >  2:475244/1‑149 (MQ=255)                                                                                                                        gTACAGCTGAACTCCCTGTCTGGCTTCTGCCTGACTAAACTGGACGTTCTGGATGGCCTGAAAGAGGTTAAACTCTGCGTGGCTTACCGTATGCCGGATGGTCGCGAAGTGACTACCACTCCGCTGGCAGCTGACGACTGGAAAGgtgt                     <  1:461756/149‑1 (MQ=255)                                                                                                                            aGCTGAACTCCCTGTCTGGCTTCTGCCTGACTAAACTGGACGTTCTGGATGGCCTGAAAGAGGTTAAACTCTGCGTGGCTTACCGTATGCCGGATGGTCGCGAAGTGACTACCACGCCGCTGGCAGCTGACGACTGGAAAGGTGTagag                 >  1:440871/1‑149 (MQ=255)                                                                                                                             gCTGAACTCCCTGTCTGGCTTCTGCCTGACTAAACTGGACGTTCTGGATGGCCTGAAAGAGGTTAAACTCTGCGTGGCTTACCGTATGCCGGATGGTCGCGAAGTGACTACCACTCCGCTGGCAGCTGACGACTGGAAAGGTGTAGAGc                >  2:368465/1‑149 (MQ=255)                                                                                                                                     cccTGTCTGGCTTCTGCCTGACTAAACTGGACGTTCTGGATGGCCTGAAAGAGGTTAAACTCTGCGTGGCTTACCGTATGCCGGATGGTCGCGAAGTGACTACCACTCCGCTGGCAGCTGACGACTGGAAAGGTGTAGAGCCGATTTAc        >  2:72617/1‑149 (MQ=255)                                                                                                                                      ccTGTCTGGCTTCTGCCTGACTAAACTGGACGTTCTAGATGGCCTGAAAGAGGTTAAACTCTGCGTGGCTTTCAGGATGCCGGATGGTCGCGAAGTGACTACCACTCCGCTGGCAGCTGACGCCTGGAAAGGTGTAGAGCCGATTTACg       >  2:104508/1‑149 (MQ=255)                                                                                                                                       cgtgcTGGCTTCTGCCGTACTAAACTTGACGTTCGTGATGGCCTGAAAGAGGTTAAACTCTGCGTGGCTTACCGTATGCCGGATGGTCGCGAAGTGACTACCGCTCCGCTGGCAGCTGACGACTGGAAAGGTGTAGAGCCGATTTACGa      <  2:633872/145‑1 (MQ=255)                                                                                                                                           cTGGCTTCTGCCTGACTAAACTGGACGTTCTGGATGGCCTGAAAGAGGTTAAACTCTGCGTGGCTTACCGTATGCCGGATGGTCGCGAAGTGACTACCACTCCGCTGGCAGCTGACGACTGGAAAGGTGTAGAGCCGATTTACGAAAcc  >  2:596600/1‑149 (MQ=255)                                                                                                                                           cTGGCTTCTGCCTGACTAAACTGGACGTTCTGGATGGCCTGAAAGAGGTTAAACTCTGCGTGGCTTACCGTATGCCGGATGGTCGCGAAGTGACTACCACTCCGCTGGCAGCTGACGACTGAAAAGGTGTAGAGCCGATTTACGAAAcc  >  1:57512/1‑149 (MQ=255)                                                                                                                                           |                                                                                                                                                     CGACCGAACTGTTTGATGAAACTGGCGAGTTCCTCTGCAAGCAGGGTAACGAATTCGGCGCAACTACGGGGCGTCGTCGTCGTACCGGCTGGCTGGACACCGTTGCCGTTCGTCGTGCGGTACAGCTGAACTCCCTGTCTGGCTTCTGCCTGACTAAACTGGACGTTCTGGATGGCCTGAAAGAGGTTAAACTCTGCGTGGCTTACCGTATGCCGGATGGTCGCGAAGTGACTACCACTCCGCTGGCAGCTGACGACTGGAAAGGTGTAGAGCCGATTTACGAAACC  >  NC_000913_3_KO_pgi/4403959‑4404245

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.