New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_KO_pgi	= 4404097	45 (1.240)	5 (0.140)	5/276	4.3	10.1%	coding (977/1299 nt)	purA	adenylosuccinate synthetase
	?	NC_000913_3_KO_pgi	= 4404109	45 (1.260)					coding (989/1299 nt)	purA	adenylosuccinate synthetase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

ACCGAACTGTTTGATGAAACTGGCGAGTTCCTCTGCAAGCAGGGTAACGAATTCGGCGCAACTACGGGGCGTCGTCGTCGTACCGGCTGGCTGGACACCGTTGCCGTTCGTCGTGCGGTACAGCTGAACTCCCTGTCTGGCTTCTGCCTGACTAAACTGGACGTTCTGGATGGCCTGAAAGAGGTTAAACTCTGCGTGGCTTACCGTATGCCGGATGGTCGCGAAGTGACTACCACTCCGCTGGCAGCTGACGACTGGAAAGGTGTAGAGCCGATTTACGAAACCATGCCGG  >  NC_000913_3_KO_pgi/4403961‑4404252                                                                                                                                                     |                                                                                                                                                accgaACTGTTTGATGAAACTGGCGAGTTCCTCTGCAAGCAGGGTAACGAATTCGGCGCAACTACGGGGCGTCGTCGTCGTACCGGCTGGCTGGACACCGTTGCCGTTCGTCTTGCGGTACAGCTGAACTCCCTGTCTGGCTTCTGCCt                                                                                                                                                 <  2:459666/149‑1 (MQ=255) accgaACTGTTTGATGAAACTGGCGAGTTCCTCTGCAAGCAGGGTAACGAATTCGGCGCAACTACGGGGCGTCGTCGTCGTACCGGCTGGCTGGACACCGTTGCCGTTCGTCGTGCGGTACAGCTGAACTCCCTGTCTGGCTTCTGCCt                                                                                                                                                 >  1:262575/1‑149 (MQ=255) accgaACTGTTTGATGAAACTGGCGAGTTCCTCTGCAAGCAGGGTAACGAATTCGGCGCAACTACGGGGCGTCGTCGTCGTACCGGCTGGCTGGACACCGTTGCCGTTCGTCGTGCGGTACAGCTGAACTCCCTGTCTGGCTTCTGCCt                                                                                                                                                 >  2:557451/1‑149 (MQ=255)   cgaACTGTTTGACGAAACGGGCGAGTTCCTCTGCAAGCAGGGTAACGAATTCGGCGCAACTACGGGGCGTCGTCGTCGTACCGGCTGGCTGGACACCGTTGCCGTTCGTCGTGCGGTACAGCTGAACTCCCTGTCTGGCTTCTGCCTGa                                                                                                                                               <  1:247667/149‑1 (MQ=255)     aaCTGTTTGATGAAACTGGCGAGTTCCTCTTCAAGCAGGGTAACGAATTCGGCGCAACTACGGGGCGTCGTCGTCGTACCGGCTGGCTGGACACCGTTGCCGTTCGTCGTGCGGTACAGCTGAACTCCCTGTCTGGCTTCTGCCTGACt                                                                                                                                             >  2:461756/1‑149 (MQ=255)        tGTTTGATGAAACTGGCGAGTTCCTCTGCAAGCAGGGTAACGAATTCGGCGCAACTACGGGGCGTCGTCGTCGTACCGGCTGGCTGGACACCGTTGCCGTTCGTCGTGCGGTACAGCTGAACTCCCTGTCTGGCTTCTGCCTGACTaaa                                                                                                                                          <  1:557451/149‑1 (MQ=255)        tGTTTGATGAAACTGGCGAGTTCCTCTGCAAGCAGGGTAACGAATTCGGCGCAACTACGGGGCGTCGTCGTCGTACCGGCTGGCTGGACACCGTTGCCGTTCGTCGTGCGGTACAGCTGAACTCCCTGTCTGGCTTCTGCCTGACTaaa                                                                                                                                          >  2:111730/1‑149 (MQ=255)         gTTTGATGAAACTGGCGAGTTCCTCTGCAAGCAGGGTAACGAATTCGGCGCAACTACGGGGCGTCGTCGTCGTACCGGCTGGCTGGACACCGTTGCCGTTCGTCGTGCGGTACAGCTGAACTCCCTGTCTGGCTTCTGCCTGACTAAAc                                                                                                                                         <  1:312300/149‑1 (MQ=255)         gTTTGATGAAACTGGCGAGTTCCTCTGCAAGCAGGGTAACGAATTCGGCGCAACTACGGGGCGTCGTCGTCGTACCGGCTGGCTGGACACCGTTGCCGTTCGTCGTGCGGTACAGCTGAACTCCCTGTCTGGCTTCTGCCTGACTAAAc                                                                                                                                         >  2:574064/1‑149 (MQ=255)                   aCTGGCGAGTTTCTCTGCAAGCAGGGTAACGAATTCGGCGCAACTACGGGGCGTCGTCGGCGTACCGGCTGGCTGGACACCGTTGCCGTTCGTCGTGCGGTACAGCTGAACTCCCTGTCTGGCTTTTGCCTGACTAATCTGGACGTTCt                                                                                                                               <  2:588786/149‑1 (MQ=255)                     tGGCGAGTTCCTCTGCAAGCAGGGTAACGAATTCGGCGCCATTCAAGTCCTTCGTCGTCGTACCGGCTGGCTGGACACCGTTGCCGTTCGTCGTGCGGTACAGCTGAACTCCCTGTCTGGCTTCTGCCTGACTAAACTGGACGTTCTgg                                                                                                                             <  1:212711/149‑1 (MQ=255)                       gCGAGTTCCTCTGCAAGCAGGGTAACGAATTCGGCGCAACTACGGGGCGTCGTCGTCGTACCGGCTGGCTGGACACCGTTGCCGTTCGTCGTGCGGTACAGCTGAACTCCCTGTCTGGCTTCTGCCTGACTAAACTGGACGTTCTGGAt                                                                                                                           >  2:536231/1‑149 (MQ=255)                            ttCCTCTGCAAGCAGGGTAACGAATTCGGCGCAACTACGGGGCGTCGTCGTCGTACCGGCTGGCTGGACACCGTTGCCGTTCGTCGTGCGGTACAGCTGAACTCCCTGTCTGGCTTCTGCCTGACTAACCTGGCCGTTCTGGATGGCCt                                                                                                                      >  1:59334/1‑149 (MQ=255)                            ttCCTCTGCAAGCAGGGTAACGAATTCGGCGCAACTACGGGGCGTCGTCGTCGTACCGGCTGGCTGGACACCGTTGCCGTTCGTCGTGCGGTACAGCTGAACTCCCTGTCTGGCTTCTGCCTGACTAAACTGGACGTTCTGGATGGCCt                                                                                                                      <  2:129744/149‑1 (MQ=255)                                   gCAAGCAGGGTAACGAATTCGGCGCAACTACGGGGCGTCGTCGTCGTACCGGCTGGCTGGACACCGTTGCCGTTCGTCGTGCGGTACAGCTGAACTCCCTGTCTGGCTTCTGCCTGACTaaa                                                                                                                                          >  1:595124/1‑122 (MQ=255)                                   gCAAGCAGGGTAACGAATTCGGCGCAACTACGGGGCGTCGTCGTCGTACCGGCTGGCTGGACACCGTTGCCGTTCGTCGTGCGGTACAGCTGAACTCCCTGTCTGGCTTCTGCCTGACTaaa                                                                                                                                          <  2:595124/122‑1 (MQ=255)                                   gCAAGCAGGGTAACGAATTCGGCGCAACTACGGGGCGTCGTCGTCGTACCGGCTGGCTGGACACCGTTGCCGTTCGTCGTGCGGTACAGCTGAACTCCCTGTCTGGCTTCTGCCTGACTAAACTGGACGTTCTGGATGGCCTGAAagag                                                                                                               <  1:111730/149‑1 (MQ=255)                                   gCAAGCAGGGTAACGAATTCGGCGCAACTACGGGGCGTCGTCGTCGTACCGGCTGGCTGGACACCGTTGCCGTTCGTCGTGCGGTACAGCTGAACTCCCTGTCTGGCTTCTGCCTGACTAAACTGGACGTTCTGGATGGCCTGAAagag                                                                                                               <  1:32012/149‑1 (MQ=255)                                      aGCATGGTAACGAACTTGGCGCAACCACGGGGCGTCGTCGTCGTACCGGCTGGCTGGACACCGTTGCCGTTCGTCGTGCGGTACAGCTGAACTCCCTGTCTGGCTTCTGCCTGACTAAACTGGACGTTCTGGATGGCCTGAAAGAGGtt                                                                                                            <  2:346765/149‑1 (MQ=255)                                                cGAATTCGGCGCAACTACGGGGCGTCGTCGTCGTACCGGCTGGCTGGACACCGTTGCCGTTCGTCGTGCGGTACAGCTGAACTCCCTGTCTGGCTTCTGCCTGACTAAACTGGACGTTCTGGATGGCCTGAAAGAGGTTAAACTCTGCg                                                                                                  >  1:521182/1‑149 (MQ=255)                                                             aCTACGGGGCGTCGTCGTCGTACCGGCTGGCTGGACACCGTTGCCGTTCGTCGTGCGGTACAGCTGAACTCCCTGTCTGGCTTCTGCCTGACTAAACTGGACGTTCTGGATGGCCTGAAAGAGGTTAAACTCTGCGTGGCTTACCGTAt                                                                                     <  1:536231/149‑1 (MQ=255)                                                               tACGGGGCGTCGTCGTCGTACCGGCTGGCTGGACACCGTTGCCGTTCGTCGTGCGGTACAGCTGAACTCCCTGTCTGGCTTCTGCCTGACTAAACTGGACGTTCt                                                                                                                               <  1:200999/105‑1 (MQ=255)                                                               tACGGGGCGTCGTCGTCGTACCGGCTGGCTGGACACCGTTGCCGTTCGTCGTGCGGTACAGCTGAACTCCCTGTCTGGCTTCTGCCTGACTAAACTGGACGTTCt                                                                                                                               >  2:200999/1‑105 (MQ=255)                                                                               cgtACCGGCTGGCTGGACACCGTTGCCGTTCGTCGTGCGGTACAGCTGAACTCCCTGTCTGGCTTCTGCCTGACTAAACTGGACGTTCTGGATGGCCTGAAAGAGGTTAAACTCTGCGTGGCTTACCGTATGCCGGATGGTCGCGAAGt                                                                   <  2:614381/149‑1 (MQ=255)                                                                               cgtACCGGCTGGCTGGACACCGTTGCCGTTCGTCGTGCGGTACAGCTGAACTCCCTGTCTGGCTTCTGCCTGACTAAACTGGACGTTCTGGATGGCCTGAAAGAGGTTAAACTCTGCGTGGCTTACCGTATGCCGGATGGTCGCGAAGt                                                                   <  2:262575/149‑1 (MQ=255)                                                                                   ccGGCTGGCTGGACACCGTTGCCGTTCGTCGTGCGGTACAGCTGAACTCCCTGTCTGGCTTCTGCCTGACTAAACTGGACGTTCTGGATGGCCTGAAAGAGGTTAAACTCTGCGTGGCTTACCGTATGCCGGATGGTCGCGACGTGACt                                                               >  2:595699/1‑149 (MQ=255)                                                                                    cGGCTGGCTGGACCCCGTCGCCGTTCGTCGTGCTGTACAGCTGAACTCCCTGGCTGGCTTCTGCCTGACTAAACTGGACGTTCTGGATGGCCTGAAAGAGGTTAAACTCTGCGTGGCTTACCGTATGCCGGATGGTCGCGAAGTGACTa                                                              <  2:290184/149‑1 (MQ=255)                                                                                               gcacCGGTGCCGTTCGTCGTGCGGTACAGCTGATCTCCCTGTCTGGCTTCTGCCTGACTAAACTGGACGTTCTGGATGGCCTGAACGAGGTTAAACTCTGCGTGGCTTACCGTATGCCGGATGGTCGCGAAGTGACTACCACTCCGCTg                                                   <  2:567533/148‑1 (MQ=255)                                                                                                  ccgtgcccgtccgtCGTGCTGTACAGCTGAACTCCGTGTCGGGCTTCTGCCTGACTAAACTGGACGTTCTGGATGGCCTGAAATAGGTTAAACGCTGCGTGGCTTACCGTATGCCGGATGGTCGCGAAGTGACTACCACTCCGCTGGCa                                                <  2:528875/143‑1 (MQ=255)                                                                                                       gCCGTTCGTCGTGCGGTACAGCTGAACTCCCTGTCTGGCTTCTGCCTGACTAAACTGGACGTTCTGGATGGCCTGAAAGAGGTTAAACTCTGCGTGGCTTACCGTATGCCGGATGGTCGCGAAGTGACTACCACTCCGCTGCCAGCtaa                                           >  2:46040/1‑147 (MQ=255)                                                                                                         cGTTCGTCGTGCGGTACAGCTGAACTCCCTGTCTGGCTTCTGCCTGACTAAACTGGACGTTCTGGATGGCCTGAAAGAGGTTAAACTCTGCGTGGCTTACCGTCTGCCGGATGGTCGCGAAGTGACTACCACTCCGCTGGCAGCTgacc                                         >  2:158179/1‑148 (MQ=255)                                                                                                          gTTCGGCGTGCGGGACAGCTGAACTCCCTGTCTGGCTTCTGCCTGACTAAACTGGACGTTCTGGATGGCCTGAAAGAGGTTAAACTCTGCGTGGCTTACCGTATGCCGGATGGTCGCGAAGTGACTACCACTCCGCTGGCAGCTgacga                                        <  2:55676/149‑1 (MQ=255)                                                                                                           ttCGTCGTGCGGTACAGCTGAACTCCCTGTCTGGCTTCTGCCTGACTAAACTGGACGTTCTGGATGGCCTGAAAGAGGTTAAACTCTGCGTGGCTTACCGTATGCCGGATGGTCGCGAAGTGACTACCACTCCGCTGGCAGCTgacgac                                       >  1:349916/1‑149 (MQ=255)                                                                                                               tcgtGCGGTACAGCTGAACTCCCTGTCTGGCTTCTGCCTGACTAAACTGGACGTTCTGGATGGCCTGAAAGAGGTTAAACTCTGCGTGGCTTACCGTATGCCGGATGGTCGCGAAGTGACTACAAACACGCTGGCAGCTGACGACGGGa                                   >  1:280427/1‑149 (MQ=255)                                                                                                                 gtGCGGTACAGCTGAACTCCCTGTCTGGCTTCTGCCTGACTAAACTGGACGTTCTGGATGGCCTGAAAGAGGTTAAACTCTGCGTGGCTTACCGTATGCCGGATGGTCGCGAAGTGACTACCACTCCGCTGGCAGCTGACGACTGGaaa                                 >  2:475244/1‑149 (MQ=255)                                                                                                                      gTACAGCTGAACTCCCTGTCTGGCTTCTGCCTGACTAAACTGGACGTTCTGGATGGCCTGAAAGAGGTTAAACTCTGCGTGGCTTACCGTATGCCGGATGGTCGCGAAGTGACTACCACTCCGCTGGCAGCTGACGACTGGAAAGgtgt                            <  1:461756/149‑1 (MQ=255)                                                                                                                          aGCTGAACTCCCTGTCTGGCTTCTGCCTGACTAAACTGGACGTTCTGGATGGCCTGAAAGAGGTTAAACTCTGCGTGGCTTACCGTATGCCGGATGGTCGCGAAGTGACTACCACGCCGCTGGCAGCTGACGACTGGAAAGGTGTagag                        >  1:440871/1‑149 (MQ=255)                                                                                                                           gCTGAACTCCCTGTCTGGCTTCTGCCTGACTAAACTGGACGTTCTGGATGGCCTGAAAGAGGTTAAACTCTGCGTGGCTTACCGTATGCCGGATGGTCGCGAAGTGACTACCACTCCGCTGGCAGCTGACGACTGGAAAGGTGTAGAGc                       >  2:368465/1‑149 (MQ=255)                                                                                                                                   cccTGTCTGGCTTCTGCCTGACTAAACTGGACGTTCTGGATGGCCTGAAAGAGGTTAAACTCTGCGTGGCTTACCGTATGCCGGATGGTCGCGAAGTGACTACCACTCCGCTGGCAGCTGACGACTGGAAAGGTGTAGAGCCGATTTAc               >  2:72617/1‑149 (MQ=255)                                                                                                                                    ccTGTCTGGCTTCTGCCTGACTAAACTGGACGTTCTAGATGGCCTGAAAGAGGTTAAACTCTGCGTGGCTTTCAGGATGCCGGATGGTCGCGAAGTGACTACCACTCCGCTGGCAGCTGACGCCTGGAAAGGTGTAGAGCCGATTTACg              >  2:104508/1‑149 (MQ=255)                                                                                                                                     cgtgcTGGCTTCTGCCGTACTAAACTTGACGTTCGTGATGGCCTGAAAGAGGTTAAACTCTGCGTGGCTTACCGTATGCCGGATGGTCGCGAAGTGACTACCGCTCCGCTGGCAGCTGACGACTGGAAAGGTGTAGAGCCGATTTACGa             <  2:633872/145‑1 (MQ=255)                                                                                                                                         cTGGCTTCTGCCTGACTAAACTGGACGTTCTGGATGGCCTGAAAGAGGTTAAACTCTGCGTGGCTTACCGTATGCCGGATGGTCGCGAAGTGACTACCACTCCGCTGGCAGCTGACGACTGGAAAGGTGTAGAGCCGATTTACGAAAcc         >  2:596600/1‑149 (MQ=255)                                                                                                                                         cTGGCTTCTGCCTGACTAAACTGGACGTTCTGGATGGCCTGAAAGAGGTTAAACTCTGCGTGGCTTACCGTATGCCGGATGGTCGCGAAGTGACTACCACTCCGCTGGCAGCTGACGACTGAAAAGGTGTAGAGCCGATTTACGAAAcc         >  1:57512/1‑149 (MQ=255)                                                                                                                                              ttCTGCCTGACTAAACTGGACGTTCTGGATGGCCTGAAAGAGGTTAAACTCTGCGTGGCTTACCGTATGCCGGATGGTCGCGAAGTGACTACCACTCCGCTGGCAGCTGACGACTGGAAAGGTGTAGAGCCGATTTACGAAACCATGcc    >  1:444497/1‑149 (MQ=255)                                                                                                                                              ttCTGCCTGACTAAACTGGACGTTCTGGATGGCCTGAAAGAGGTTAAACTCTGCGTGGCTTACAGTAAGCCGGATGGTCCCGAAGTGACTACCACTCCGCTGGCAGCTGACGACTGGATAGGTGTAGAGCCGATTTACGAAACCATGcc    >  1:152814/1‑149 (MQ=255)                                                                                                                                              ttCTGCCTGACTAAACTGGACGTTCTGGATGGACTGAAAGAGGTTAAACTCTGCGTGGCTTACCGTATGCCGGATGGTAGCGAAGTCACTGCCACTCCGCTAGCAGCTGAAGACTGGAAAGGTGTGGAGCAGATTTACCAAACCATccc    >  2:378439/1‑149 (MQ=255)                                                                                                                                                cTGCCTGACTAAACTGGACGTTCTGGATGGCCTGAAAGAGGTTAAACTCTGCGTGGCTTACCGTATGCCGGATGGTCGCGAAGTGACTACCACTCCGCTGGCAGCTGACGACTGGAAAGGTGTAGAGCCGATTTACGAAACCATGCCgg  <  1:574064/149‑1 (MQ=255)                                                                                                                                                    cTGACTAAACTGGACGTTCTGGATGGCCTGAAAGAGGTTAAACTCTGCGTGGCTTACCGTATGCCGGATGGTCGCGAAGTGACTACCACTCCg                                                      <  2:193590/93‑1 (MQ=255)                                                                                                                                                    cTGACTAAACTGGACGTTCTGGATGGCCTGAAAGAGGTTAAACTCTGCGTGGCTTACCGTATGCCGGATGGTCGCGAAGTGACTACCACTCCg                                                      >  1:193590/1‑93 (MQ=255)                                                                                                                                                     |                                                                                                                                                ACCGAACTGTTTGATGAAACTGGCGAGTTCCTCTGCAAGCAGGGTAACGAATTCGGCGCAACTACGGGGCGTCGTCGTCGTACCGGCTGGCTGGACACCGTTGCCGTTCGTCGTGCGGTACAGCTGAACTCCCTGTCTGGCTTCTGCCTGACTAAACTGGACGTTCTGGATGGCCTGAAAGAGGTTAAACTCTGCGTGGCTTACCGTATGCCGGATGGTCGCGAAGTGACTACCACTCCGCTGGCAGCTGACGACTGGAAAGGTGTAGAGCCGATTTACGAAACCATGCCGG  >  NC_000913_3_KO_pgi/4403961‑4404252

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.