New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_KO_pgi	3654539 =	33 (0.910)	4 (0.120)	4/258	4.5	11.1%	intergenic (+12/‑144)	slp/dctR	outer membrane lipoprotein/Putative LuxR family repressor for dicarboxylate transport
	?	NC_000913_3_KO_pgi	3654568 =	34 (1.020)					intergenic (+41/‑115)	slp/dctR	outer membrane lipoprotein/Putative LuxR family repressor for dicarboxylate transport
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CTGTGGGATTACGGCTATGGTGCATTCTGGCCGGAACCGGGCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGTCCGCACCAGGAGTCGGTTATGTTTCTTATAATTACCAGGGATACGATGTT  >  NC_000913_3_KO_pgi/3654426‑3654714                                                                                                                                               |                                                                                                                                                   gtgtgGGATTACGGCTATGATGCATTCTGGCAGGAACCGGGCGGGGGTGCGCCTTACTACACCAAAGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCGACGCAAGATGCAGATACTTTCTCAGGAACTGCATCATGCAtt                                                                                                                                              >  2:338379/2‑149 (MQ=255)  tgtgGGATTACGGCTATGGTGCATTCTGGCCGGAACCGGGCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTa                                                                                                                                             >  1:135857/1‑149 (MQ=255)               cTATGGTGCATTCTGGCCGGAACCGGGCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATa                                                                                                                                <  1:132927/149‑1 (MQ=255)                     tGCATTCTGGCCGGAACCGGGATGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGt                                                                                                                          >  2:461858/1‑149 (MQ=255)                                   aaCCGGGCTGGGGGGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACtattat                                                                                                            <  1:225504/149‑1 (MQ=255)                                       gggCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACtattattatt                                                                                                        <  1:449793/149‑1 (MQ=255)                                                          tacACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATacac                                                                                                                             >  1:401823/1‑109 (MQ=255)                                                          tacACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATacac                                                                                                                             <  2:401823/109‑1 (MQ=255)                                                           acacCAATACGGTGAGTCACGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCGGCATCATGCATTATAGCAAATTAAGACACAGTAAGCTAACTAATTGTCTTATGAGCCCTGTCCTGTTAAt                                                                                    >  2:540206/1‑149 (MQ=255)                                                                aaTGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATtata                                                                                                     >  1:152143/1‑127 (MQ=255)                                                                aaTGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATtata                                                                                                     <  2:152143/127‑1 (MQ=255)                                                                     ggTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTAACTTTGGc                                                                          >  1:248828/1‑149 (MQ=255)                                                                       tGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGAGGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATATGCCCTGTCCTGTTAATTACCTTTGGCaa                                                                        >  1:290265/1‑149 (MQ=255)                                                                              ggTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGAtt                                                                 >  2:492034/1‑149 (MQ=255)                                                                              ggTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGAtt                                                                 <  1:492034/149‑1 (MQ=255)                                                                              ggTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGAtt                                                                 >  2:269114/1‑149 (MQ=255)                                                                                   cacCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCGTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATaaa                                                            <  2:294560/149‑1 (MQ=255)                                                                                    acCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATGATAAAg                                                           >  1:481724/1‑149 (MQ=255)                                                                                         aGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAAt                                                      <  2:472644/149‑1 (MQ=255)                                                                                                      accccaccGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCTTGTCCTGTTAATTACCTTTGGCAAACTGATTATAATGTTAATGTCCGCACCAGGa                                         <  2:216400/146‑1 (MQ=255)                                                                                                              gAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGAt                                                                  <  2:549283/116‑1 (MQ=255)                                                                                                              gAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTGAATTACCTTTGGCAAACTGAt                                                                  >  1:549283/1‑116 (MQ=255)                                                                                                              aaaaCATTCAGATGCCTTCTCAGGATCTGCATCATGCATTACATCGAATTAATACACAGTAATCTAACTATTATTATTCTAAGCCCGATCCTGTTAATTACCTTTGGCAATCTGATTATGAAGTTAATGTCCGCACCAGGAGTCGGTTa                                 <  1:540206/148‑1 (MQ=255)                                                                                                               aaaGATGCAGATGCTTTCTCAGCATCTGCAGCATGCATTACATCAAATTAATACCCAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGTCCGCACCAGGAGTCGGTTAt                                <  1:523012/149‑1 (MQ=255)                                                                                                                   atgcagatgcTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGTCCGCCCCAGGAGTCGGTTATGttt                            >  2:488316/1‑149 (MQ=255)                                                                                                                       agatgcTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGTGTATAATGTTAATGTCCGCACCAGGAGTCGGTTATGTTTCttc                        >  1:283807/1‑148 (MQ=255)                                                                                                                        gatgcTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAAt                                                                                    <  2:28678/88‑1 (MQ=255)                                                                                                                        gatgcTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAAt                                                                                    >  1:28678/1‑88 (MQ=255)                                                                                                                         atgcTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTAc                                                                                 <  2:121172/90‑1 (MQ=255)                                                                                                                         atgcTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTAc                                                                                 >  1:121172/1‑90 (MQ=255)                                                                                                                         atgcTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGTCCGCACCAGGAGTCGGTTATGTTTCTtata                      >  1:215396/1‑149 (MQ=255)                                                                                                                             tttCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGTCCGCACCAGGAGTCGGTTATGTTTCTTATAATTa                  >  2:352935/1‑149 (MQ=255)                                                                                                                                 tcAGCATCTGCATCATGCATTACATAAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGTCCGCACCAGGAGTCGGTTATGTTTCTTATAATTACCAg              <  2:135857/149‑1 (MQ=255)                                                                                                                                  cAGCATCTTCATCATGCATTACATCAAATTAATACACCGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGTCCGCACCAGGAGTCGGTTATGTTTCTTATAATTACCAgg             <  1:557537/149‑1 (MQ=255)                                                                                                                                           catcatGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAACGTTACTGTCCGCACCAGGAGTCGGTTATGTTTCTTATAATTACCAGGGATACGATg    >  2:482699/1‑149 (MQ=255)                                                                                                                                           catcatGCAGTACATCAAATTAATACACAGTAAGCTAACTAGTATTATTATAAGCCCTGTCCTGTTAATTACCGTTGGCAAACTGATTATAAAGATAATGTCCGCACCAGGAGTGGGTTATGTTTCTTATAATTACCAGGGATACGAta    >  2:409208/1‑148 (MQ=255)                                                                                                                                             tcatGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGTCCGCACCAGGAGTCGGTTATGTTTCTTATAATTACCAGGGATACGATGtt  <  2:178733/149‑1 (MQ=255)                                                                                                                                               |                                                                                                                                                   CTGTGGGATTACGGCTATGGTGCATTCTGGCCGGAACCGGGCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGTCCGCACCAGGAGTCGGTTATGTTTCTTATAATTACCAGGGATACGATGTT  >  NC_000913_3_KO_pgi/3654426‑3654714

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.