breseq version 0.32.0b
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Predicted mutations | |||||
---|---|---|---|---|---|
evidence | position | mutation | annotation | gene | description |
RA | 982,565 | T→A | intergenic (‑127/+476) | ompF ← / ← asnS | outer membrane porin 1a (Ia;b;F)/asparaginyl tRNA synthetase |
RA | 1,104,736 | C→T | intergenic (‑339/‑55) | opgC ← / → opgG | osmoregulated periplasmic glucan succinylation membrane protein/osmoregulated periplasmic glucan (OPG) biosynthesis periplasmic protein |
RA | 1,111,161 | C→A | R293L (CGT→CTT) | lpxL ← | lauryl‑acyl carrier protein (ACP)‑dependent acyltransferase |
RA | 1,123,224 | A→T | intergenic (+39/‑71) | yceM → / → murJ | putative oxidoreductase/putative peptidoglycan lipid II flippase |
RA | 1,130,039 | G→A | intergenic (+26/‑146) | flgF → / → flgG | flagellar component of cell‑proximal portion of basal‑body rod/flagellar component of cell‑distal portion of basal‑body rod |
RA | 1,154,028 | G→A | G235D (GGC→GAC) | ptsG → | fused glucose‑specific PTS enzymes: IIB component/IIC component |
RA | 2,083,827 | G→C | R52P (CGC→CCC) | hisG → | ATP phosphoribosyltransferase |
JC JC | 2,400,257 | IS5 (+) +4 bp | intergenic (‑137/‑780) | lrhA ← / → alaA | transcriptional repressor of flagellar, motility and chemotaxis genes/valine‑pyruvate aminotransferase 2 |
RA | 2,420,353 | A→G | intergenic (‑86/+135) | hisJ ← / ← argT | histidine/lysine/arginine/ornithine transporter subunit/lysine/arginine/ornithine transporter subunit |
RA | 2,420,394 | G→T | intergenic (‑127/+94) | hisJ ← / ← argT | histidine/lysine/arginine/ornithine transporter subunit/lysine/arginine/ornithine transporter subunit |
JC JC | 3,178,254 | IS5 (+) +4 bp | coding (56‑59/291 nt) | yqiC → | BMFP family putative fusogenic protein |
MC JC | 3,360,110 | Δ10,684 bp | IS5‑mediated | [yhcE]–sspA | [yhcE], yhcF, yhcG, yhcH, nanK, nanE, nanT, nanA, nanR, dcuD, sspB, sspA |
Unassigned missing coverage evidence | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|
seq id | start | end | size | ←reads | reads→ | gene | description | |||
* | * | ÷ | CP009273 | 2084543 | 2085911 | 1369 | 45 [41] | [43] 47 | [hisG]–[hisC] | [hisG], hisD, [hisC] |
Unassigned new junction evidence | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | CP009273 | 360104 = | NA (NA) | 127 (1.460) | 84/288 | 0.0 | NA | intergenic (‑38/+557) | lacZ/lacI | pseudogene, truncated/DNA‑binding transcriptional repressor |
? | CP009273 | = 360286 | NA (NA) | intergenic (‑220/+375) | lacZ/lacI | pseudogene, truncated/DNA‑binding transcriptional repressor | |||||
* | ? | CP009273 | 1203246 = | 16 (0.180) | 56 (0.730) | 45/256 | 1.0 | 78.2% | coding (290/630 nt) | stfP | e14 prophage; uncharacterized protein |
? | CP009273 | 1205075 = | 17 (0.220) | pseudogene (1/501 nt) | stfE | pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related | |||||
* | ? | CP009273 | = 1203261 | 16 (0.180) | 60 (0.780) | 43/256 | 1.2 | 79.3% | coding (305/630 nt) | stfP | e14 prophage; uncharacterized protein |
? | CP009273 | = 1205058 | 17 (0.220) | pseudogene (18/501 nt) | stfE | pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related |