Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	CP009273	3,270,632	(TTCAACA)2→3	coding (272/336 nt)	prlF →	antitoxin of the SohA(PrlF)‑YhaV toxin‑antitoxin system

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	CP009273	= 3270616	1 (0.020)	38 (0.850) +CATTCAA	35/250	0.2	97.4%	coding (256/336 nt)	prlF	antitoxin of the SohA(PrlF)‑YhaV toxin‑antitoxin system
	?	CP009273	3270617 =	1 (0.020)					coding (257/336 nt)	prlF	antitoxin of the SohA(PrlF)‑YhaV toxin‑antitoxin system

AGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTATGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  CP009273/3270476‑3270616 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTAC  >  CP009273/3270617‑3270761                                                                                                                                              |||||||                                                                                                                                                  AGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTATGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCTCCCACTCAAC                                                                                                                                                  >  1:305024/1‑149    ACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTATGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCCATTCAACATT                                                                                                                                               <  2:749331/149‑1      AGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTATGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCCATTCAACATTCA                                                                                                                                             <  2:261600/149‑1         ATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTATGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCCATTCAAAATTCAACA                                                                                                                                          >  2:594273/1‑149                  GAAATTCTGCCTGGTGGGCAAGTATTTATGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCCATTCAACAGTCAACATTCAACAAG                                                                                                                                 >  1:723984/1‑149                  GAAATTCTGCCTGGTGGGCAAGTATTTATGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAAAAACCCGCAAAAAACTCGTCCAGTCCACGTACAACATTCAACAAG                                                                                                                                 >  1:313532/1‑149                    AATTCTGCCTGGTGGGCAAGTATTTATGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCCGTTCAACATTCAACATTAAACAAGGA                                                                                                                               >  1:212284/1‑149                    AATTCTGCCTGCTGGGCAGGGATTTATGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCCATTCAACATTCAACATTCAACAAGGA                                                                                                                               <  2:67939/149‑1                     ATTCTGCCTGGTGGGCAAGTATTTATGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCCATTCAACATTCAACATTCAACAAGGAA                                                                                                                              >  2:500335/1‑149                        CTGCCTGGTGGGCAAGTATTTATGTGACCACTGGAAGACGAACAAGAAGATCATATTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCCATTCAACATTCAACATTCAACAAGGAAAGA                                                                                                                           <  2:335299/149‑1                         TGCCTGGTGGGCAAGTATTTATGTGCCGACTGGGGGATGAACAGGAGGATCATACTATGAATGCCTTTTTGCGTTTTCTGGATGCAGATATCCAGAACACCCCGCAAAAAACTCGGCCATTCAACATTCAACATTGCACAAGGAAAGAA                                                                                                                          >  2:145660/1‑149                            CTGGTGGGCAAGTATTTATGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCCATTCAACATTCAACATTCAACAAGGAAAGAAACT                                                                                                                       >  1:774705/1‑149                             TGGTGGGCAAGTATTTATGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCCATTCAACATTCAACATTCAACAAGGAAAGAAACTT                                                                                                                      <  2:673857/149‑1                                    CAAGTATTTATGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCCATTCAACATTCAACATTCAACAAGGAAAGAAACTTGTCGCTG                                                                                                               >  2:391767/1‑149                                              TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCCATTCAACATTCAACATTCACCAAGGAAAGAAACTTGTCGCTGGCATGGACGT                                                                                                     >  2:190418/1‑149                                              TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCCAACCAACATTCAACAATAAACAAAGAAAGAAAATAATAACTAGCATGGACGT                                                                                                     >  2:162874/1‑149                                                 GCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCCATTCAACATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAA                                                                                                  >  1:537662/1‑149                                                   cgtCTGGGAGATGAACAGGGGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCCATTCAACATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACA                                                                                                <  1:148678/146‑1                                                        cgtAGATGAACAACAGGATCATACTAACAATGCATTTTTCCATTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAAATCGTCCATTCAACATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGAT                                                                                           <  2:66077/146‑1                                                           AGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCCATTCAACATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACCTTGATGAT                                                                                        >  2:307361/1‑149                                                            GATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCCATTCAACATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATG                                                                                       >  2:709399/1‑149                                                               GAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCCATTCAACATTCAAGATTCAACAAGGAAAGAAACTTGTCGCTGACATGGACGTCAACATTGATGATGAGA                                                                                    >  2:407482/1‑149                                                                     GAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCCATTCAACATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCG                                                                              >  2:625642/1‑149                                                                     GAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCCATTCAACATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCG                                                                              >  2:58700/1‑149                                                                            ATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGACATCCAGAACAACCCGCAAAAAACTCGTCCATTCAACATTCAACATTCAACAAGGAAAGAAACTTGTCGCTTGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGA                                                                       <  1:368097/149‑1                                                                               CTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCCATTCAACATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATA                                                                    <  1:625642/149‑1                                                                                 ATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAACCTCGTCCATTCAACATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATAAGATTGACGACGACAAATAAT                                                                  >  2:249287/1‑149                                                                                 ATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCCATTCAACATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAAATTGGCGACGACGAATAAT                                                                  >  2:83280/1‑149                                                                                    AATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCCATTCAACATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGA                                                               <  2:313532/149‑1                                                                                        CATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCCATTCAACATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTT                                                           <  2:774705/149‑1                                                                                        CATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCCATTCAACATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTT                                                           <  1:678976/149‑1                                                                                           TTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAGCTCGTCCATTCAACATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGACATGGACGACAACATTGATGATGAGATTGGCGCCGACGAATAATGGACTTTCCA                                                        >  1:406020/1‑149                                                                                                      CTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCCATTCAACATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAA                                             >  1:123539/1‑149                                                                                                          agtgatatatagAGAACAACCCGGAAAAGACTCGTCCATTCAACATTCAATATTGAGCAAGGAAAGAAACTGGTCGCTGGCATGGACGTCAACATTGAGGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAATGGT                                         <  1:664456/137‑1                                                                                                          ATGCAGATATCCAGAACAACCCGCAAAAAACTCGACCATTCAACATTCAACATTCAACAGGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAGTAATGGATTTTCCACAAAGGGTTAATGGT                                         >  1:544306/1‑149                                                                                                            GCAGATATCCAGAACAACCCGCAAAAAACTCGTCCATTCAACATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAAAAATGGATTTTCCACAAAGGGTTAATGGTTG                                       >  2:28435/1‑149                                                                                                                      AGAACAACCCGCAAAAAACTCGTCCATTCAACATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATAT                             >  2:750408/1‑149                                                                                                                            ACCCGCAAAAAACTCGTCCATTCAACATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCAT                       <  2:363452/149‑1                                                                                                                                  AAAAAACTCGTCCATTCAACATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGT                 <  2:427881/149‑1                                                                                                                                   AAAAACTCGTCCATTCAACATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTT                >  1:661444/1‑149                                                                                                                                   AAAAACTCGTCCATTCAACATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAGTGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTT                <  2:380552/149‑1                                                                                                                                       ACTCTTCCATTCAACATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCA            <  1:500335/149‑1                                                                                                                                        CTCGTCCATTCAACATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAG           <  1:524387/149‑1                                                                                                                                         TCGTCCATTCAACATTCAACATTAAAGAATGAAAGAAACTTGCCGCTGGCATGGACGTCAACATTGAGGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGG          <  2:257343/149‑1                                                                                                                                            TCCATTCAACATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAA       <  2:646004/149‑1                                                                                                                                              CATTCAACATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGGGATTGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACC     >  2:88385/1‑149                                                                                                                                                 TCAACATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTAC  >  2:75555/1‑149                                                                                                                                              |||||||                                                                                                                                                  AGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTATGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  CP009273/3270476‑3270616 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTAC  >  CP009273/3270617‑3270761

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.