Predicted mutation
evidence seq id position mutation annotation gene description
JC CP009273 3,270,632 (TTCAACA)2→3 coding (272/336 nt) prlF → antitoxin of the SohA(PrlF)‑YhaV toxin‑antitoxin system

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009273 = 32706161 (0.020)38 (0.850)
+CATTCAA
35/250 0.2 97.4% coding (256/336 nt) prlF antitoxin of the SohA(PrlF)‑YhaV toxin‑antitoxin system
?CP009273 3270617 = 1 (0.020)coding (257/336 nt) prlF antitoxin of the SohA(PrlF)‑YhaV toxin‑antitoxin system

AGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTATGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCCATTCAACATTCAA‑‑‑‑‑‑‑CAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAA  >  CP009273/3270476‑3270756
                                                                                                                                            |                                                                                                                                                   
aggACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTATGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCTCCcactcaac                                                                                                                                             >  1:305024‑M1/1‑141 (MQ=255)
   aCAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTATGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCcattcaacatt                                                                                                                                          <  2:749331‑M1/149‑12 (MQ=255)
     aGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTATGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCcattcaacattca                                                                                                                                        <  2:261600‑M1/149‑14 (MQ=255)
        attcattACGAAATTCTGCCTGGTGGGCAAGTATTTATGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCcattcaaaattcaaca                                                                                                                                     >  2:594273‑M1/1‑133 (MQ=255)
                 gAAATTCTGCCTGGTGGGCAAGTATTTATGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCcattcaacagtcaacattcaacaag                                                                                                                            >  1:723984‑M1/1‑124 (MQ=255)
                 gAAATTCTGCCTGGTGGGCAAGTATTTATGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAAAAACCCGCAAAAAACTCGTCcagtccacgtacaacattcaacaag                                                                                                                            >  1:313532‑M1/1‑124 (MQ=255)
                   aaTTCTGCCTGGTGGGCAAGTATTTATGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCcgttcaacattcaacattaaacaagga                                                                                                                          >  1:212284‑M1/1‑122 (MQ=255)
                   aaTTCTGCCTGCTGGGCAGGGATTTATGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCcattcaacattcaacattcaacaagga                                                                                                                          <  2:67939‑M1/149‑28 (MQ=255)
                    aTTCTGCCTGGTGGGCAAGTATTTATGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCcattcaacattcaacattcaacaaggaa                                                                                                                         >  2:500335‑M1/1‑121 (MQ=255)
                       cTGCCTGGTGGGCAAGTATTTATGTGACCACTGGAAGACGAACAAGAAGATCATATTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCcattcaacattcaacattcaacaaggaaaga                                                                                                                      <  2:335299‑M1/149‑32 (MQ=255)
                        tGCCTGGTGGGCAAGTATTTATGTGCCGACTGGGGGATGAACAGGAGGATCATACTATGAATGCCTTTTTGCGTTTTCTGGATGCAGATATCCAGAACACCCCGCAAAAAACTCGGCcattcaacattcaacattgcacaaggaaagaa                                                                                                                     >  2:145660‑M1/1‑117 (MQ=255)
                           cTGGTGGGCAAGTATTTATGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCcattcaacattcaacattcaacaaggaaagaaact                                                                                                                  >  1:774705‑M1/1‑114 (MQ=255)
                            tggtggGCAAGTATTTATGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCcattcaacattcaacattcaacaaggaaagaaactt                                                                                                                 <  2:673857‑M1/149‑37 (MQ=255)
                                   cAAGTATTTATGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCcattcaacattcaacattcaacaaggaaagaaacttgtcgctg                                                                                                          >  2:391767‑M1/1‑106 (MQ=255)
                                             tgtgCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCcattcaacattcaacattcaccaaggaaagaaacttgtcgctggcatggacgt                                                                                                >  2:190418‑M1/1‑96 (MQ=255)
                                             tgtgCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCcaaccaacattcaacaataaacaaagaaagaaaataataactagcatggacgt                                                                                                >  2:162874‑M1/1‑96 (MQ=255)
                                                gCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCcattcaacattcaacattcaacaaggaaagaaacttgtcgctggcatggacgtcaa                                                                                             >  1:537662‑M1/1‑93 (MQ=255)
                                                  cgtctggGAGATGAACAGGGGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCcattcaacattcaacattcaacaaggaaagaaacttgtcgctggcatggacgtcaaca                                                                                           <  1:148678‑M1/146‑59 (MQ=255)
                                                       cgtagatgaACAACAGGATCATACTAACAATGCATTTTTCCATTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAAATCGTCcattcaacattcaacattcaacaaggaaagaaacttgtcgctggcatggacgtcaacattgat                                                                                      <  2:66077‑M1/146‑64 (MQ=255)
                                                          agaTGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCcattcaacattcaacattcaacaaggaaagaaacttgtcgctggcatggacgtcaaccttgatgat                                                                                   >  2:307361‑M1/1‑83 (MQ=255)
                                                           gaTGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCcattcaacattcaacattcaacaaggaaagaaacttgtcgctggcatggacgtcaacattgatgatg                                                                                  >  2:709399‑M1/1‑82 (MQ=255)
                                                              gAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCcattcaacattcaagattcaacaaggaaagaaacttgtcgctgacatggacgtcaacattgatgatgaga                                                                               >  2:407482‑M1/1‑79 (MQ=255)
                                                                    gaggaTCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCcattcaacattcaacattcaacaaggaaagaaacttgtcgctggcatggacgtcaacattgatgatgagattggcg                                                                         >  2:625642‑M1/1‑73 (MQ=255)
                                                                    gaggaTCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCcattcaacattcaacattcaacaaggaaagaaacttgtcgctggcatggacgtcaacattgatgatgagattggcg                                                                         >  2:58700‑M1/1‑73 (MQ=255)
                                                                           aTACTATGAATGCATTTTTGCGTTTTCTGGATGCAGACATCCAGAACAACCCGCAAAAAACTCGTCcattcaacattcaacattcaacaaggaaagaaacttgtcgcttgcatggacgtcaacattgatgatgagattggcgacgacga                                                                  <  1:368097‑M1/149‑84 (MQ=255)
                                                                              cTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCcattcaacattcaacattcaacaaggaaagaaacttgtcgctggcatggacgtcaacattgatgatgagattggcgacgacgaata                                                               <  1:625642‑M1/149‑87 (MQ=255)
                                                                                aTGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAACCTCGTCcattcaacattcaacattcaacaaggaaagaaacttgtcgctggcatggacgtcaacattgatgataagattgacgacgacaaataat                                                             >  2:249287‑M1/1‑61 (MQ=255)
                                                                                aTGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCcattcaacattcaacattcaacaaggaaagaaacttgtcgctggcatggacgtcaacattgatgatgaaattggcgacgacgaataat                                                             >  2:83280‑M1/1‑61 (MQ=255)
                                                                                   aaTGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCcattcaacattcaacattcaacaaggaaagaaacttgtcgctggcatggacgtcaacattgatgatgagattggcgacgacgaataatgga                                                          <  2:313532‑M1/149‑92 (MQ=255)
                                                                                       cATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCcattcaacattcaacattcaacaaggaaagaaacttgtcgctggcatggacgtcaacattgatgatgagattggcgacgacgaataatggatttt                                                      <  1:678976‑M1/149‑96 (MQ=255)
                                                                                       cATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCcattcaacattcaacattcaacaaggaaagaaacttgtcgctggcatggacgtcaacattgatgatgagattggcgacgacgaataatggatttt                                                      <  2:774705‑M1/149‑96 (MQ=255)
                                                                                          ttttGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAGCTCGTCcattcaacattcaacattcaacaaggaaagaaacttgtcgctgacatggacgacaacattgatgatgagattggcgccgacgaataatggactttcca                                                   >  1:406020‑M1/1‑51 (MQ=255)
                                                                                                     cTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCcattcaacattcaacattcaacaaggaaagaaacttgtcgctggcatggacgtcaacattgatgatgagattggcgacgacgaataatggattttccacaaagggttaa                                        >  1:123539‑M1/1‑40 (MQ=255)
                                                                                                         agtgatatatagagaacaacccggaAAAGACTCGTCcattcaacattcaatattgagcaaggaaagaaactggtcgctggcatggacgtcaacattgaggatgagattggcgacgacgaataatggattttccacaaagggttaatggt                                    <  1:664456‑M1/137‑114 (MQ=255)
                                                                                                         aagaagctatCCAGGACAACCCGCAAAAAACTCGTCCATTCAACATTCAACATTCACCAAGGAAGGAAACTTGTCGCTGGCCTGGACGTCAGCATTGATGATGGGGTTGGCCACGACGAATAAGGGATTGTCCGCAGAAGGATAATGGt                                    >  1:133973/8‑149 (MQ=255)
                                                                                                         aTGCAGATATCCAGAACAACCCGCAAAAAACTCGACcattcaacattcaacattcaacagggaaagaaacttgtcgctggcatggacgtcaacattgatgatgagattggcgacgacgagtaatggattttccacaaagggttaatggt                                    >  1:544306‑M1/1‑36 (MQ=255)
                                                                                                           gCAGATATCCAGAACAACCCGCAAAAAACTCGTCcattcaacattcaacattcaacaaggaaagaaacttgtcgctggcatggacgtcaacattgatgatgagattggcgacgacgaaaaatggattttccacaaagggttaatggttg                                  >  2:28435‑M1/1‑34 (MQ=255)
                                                                                                                     aGAACAACCCGCAAAAAACTCGTCcattcaacattcaacattcaacaaggaaagaaacttgtcgctggcatggacgtcaacattgatgatgagattggcgacgacgaataatggattttccacaaagggttaatggttgggcgctatat                        >  2:750408‑M1/1‑24 (MQ=255)
                                                                                                                           acCCGCAAAAAACTCGTCcattcaacattcaacattcaacaaggaaagaaacttgtcgctggcatggacgtcaacattgatgatgagattggcgacgacgaataatggattttccacaaagggttaatggttgggcgctatatgctcat                  <  2:363452‑M1/149‑132 (MQ=255)
                                                                                                                                 aaaaaaCTCGTCcattcaacattcaacattcaacaaggaaagaaacttgtcgctggcatggacgtcaacattgatgatgagattggcgacgacgaataatggattttccacaaagggttaatggttgggcgctatatgctcatccctgt            <  2:427881‑M1/149‑138 (MQ=255)
                                                                                                                                  aaaaaCTCGTCcattcaacattcaacattcaacaaggaaagaaacttgtcgctggcatggacgtcaacattgatgatgagattggcgacgacgaataatggattttccacaaagggttaatggttgggcgctatatgctcatccctgtt           >  1:661444‑M1/1‑11 (MQ=255)
                                                                                                                                  aaaaaCTCGTCcattcaacattcaacattcaacaaggaaagaaacttgtcgctggcatggacgtcaacattgatgatgagagtggcgacgacgaataatggattttccacaaagggttaatggttgggcgctatatgctcatccctgtt           <  2:380552‑M1/149‑139 (MQ=255)
                                                                                                                                      aCTCTTCcattcaacattcaacattcaacaaggaaagaaacttgtcgctggcatggacgtcaacattgatgatgagattggcgacgacgaataatggattttccacaaagggttaatggttgggcgctatatgctcatccctgttttca       <  1:500335‑M1/149‑143 (MQ=255)
                                                                                                                                       cTCGTCcattcaacattcaacattcaacaaggaaagaaacttgtcgctggcatggacgtcaacattgatgatgagattggcgacgacgaataatggattttccacaaagggttaatggttgggcgctatatgctcatccctgttttcag      <  1:524387‑M1/149‑144 (MQ=255)
                                                                                                                                        tCGTCcattcaacattcaacattaaagaatgaaagaaacttgccgctggcatggacgtcaacattgaggatgagattggcgacgacgaataatggattttccacaaagggttaatggttgggcgctatatgctcatccctgttttcagg     <  2:257343‑M1/149‑145 (MQ=255)
                                                                                                                                           tCcattcaacattcaacattcaacaaggaaagaaacttgtcgctggcatggacgtcaacattgatgatgagattggcgacgacgaataatggattttccacaaagggttaatggttgggcgctatatgctcatccctgttttcaggaaa  <  2:646004‑M1/149‑148 (MQ=255)
                                                                                                                                            |                                                                                                                                                   
AGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTATGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCCATTCAACATTCAA‑‑‑‑‑‑‑CAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAA  >  CP009273/3270476‑3270756

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.