Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
JC | CP009273 | 3,270,632 | (TTCAACA)2→3 | coding (272/336 nt) | prlF → | antitoxin of the SohA(PrlF)‑YhaV toxin‑antitoxin system |
New junction evidence | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | CP009273 | = 3270616 | 1 (0.020) | 38 (0.850) +CATTCAA |
35/250 | 0.2 | 97.4% | coding (256/336 nt) | prlF | antitoxin of the SohA(PrlF)‑YhaV toxin‑antitoxin system |
? | CP009273 | 3270617 = | 1 (0.020) | coding (257/336 nt) | prlF | antitoxin of the SohA(PrlF)‑YhaV toxin‑antitoxin system |
AGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTATGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCCATTCAACATTCAA‑‑‑‑‑‑‑CAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAA > CP009273/3270476‑3270756 | aggACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTATGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCTCCcactcaac > 1:305024‑M1/1‑141 (MQ=255) aCAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTATGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCcattcaacatt < 2:749331‑M1/149‑12 (MQ=255) aGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTATGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCcattcaacattca < 2:261600‑M1/149‑14 (MQ=255) attcattACGAAATTCTGCCTGGTGGGCAAGTATTTATGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCcattcaaaattcaaca > 2:594273‑M1/1‑133 (MQ=255) gAAATTCTGCCTGGTGGGCAAGTATTTATGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCcattcaacagtcaacattcaacaag > 1:723984‑M1/1‑124 (MQ=255) gAAATTCTGCCTGGTGGGCAAGTATTTATGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAAAAACCCGCAAAAAACTCGTCcagtccacgtacaacattcaacaag > 1:313532‑M1/1‑124 (MQ=255) aaTTCTGCCTGGTGGGCAAGTATTTATGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCcgttcaacattcaacattaaacaagga > 1:212284‑M1/1‑122 (MQ=255) aaTTCTGCCTGCTGGGCAGGGATTTATGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCcattcaacattcaacattcaacaagga < 2:67939‑M1/149‑28 (MQ=255) aTTCTGCCTGGTGGGCAAGTATTTATGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCcattcaacattcaacattcaacaaggaa > 2:500335‑M1/1‑121 (MQ=255) cTGCCTGGTGGGCAAGTATTTATGTGACCACTGGAAGACGAACAAGAAGATCATATTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCcattcaacattcaacattcaacaaggaaaga < 2:335299‑M1/149‑32 (MQ=255) tGCCTGGTGGGCAAGTATTTATGTGCCGACTGGGGGATGAACAGGAGGATCATACTATGAATGCCTTTTTGCGTTTTCTGGATGCAGATATCCAGAACACCCCGCAAAAAACTCGGCcattcaacattcaacattgcacaaggaaagaa > 2:145660‑M1/1‑117 (MQ=255) cTGGTGGGCAAGTATTTATGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCcattcaacattcaacattcaacaaggaaagaaact > 1:774705‑M1/1‑114 (MQ=255) tggtggGCAAGTATTTATGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCcattcaacattcaacattcaacaaggaaagaaactt < 2:673857‑M1/149‑37 (MQ=255) cAAGTATTTATGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCcattcaacattcaacattcaacaaggaaagaaacttgtcgctg > 2:391767‑M1/1‑106 (MQ=255) tgtgCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCcattcaacattcaacattcaccaaggaaagaaacttgtcgctggcatggacgt > 2:190418‑M1/1‑96 (MQ=255) tgtgCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCcaaccaacattcaacaataaacaaagaaagaaaataataactagcatggacgt > 2:162874‑M1/1‑96 (MQ=255) gCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCcattcaacattcaacattcaacaaggaaagaaacttgtcgctggcatggacgtcaa > 1:537662‑M1/1‑93 (MQ=255) cgtctggGAGATGAACAGGGGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCcattcaacattcaacattcaacaaggaaagaaacttgtcgctggcatggacgtcaaca < 1:148678‑M1/146‑59 (MQ=255) cgtagatgaACAACAGGATCATACTAACAATGCATTTTTCCATTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAAATCGTCcattcaacattcaacattcaacaaggaaagaaacttgtcgctggcatggacgtcaacattgat < 2:66077‑M1/146‑64 (MQ=255) agaTGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCcattcaacattcaacattcaacaaggaaagaaacttgtcgctggcatggacgtcaaccttgatgat > 2:307361‑M1/1‑83 (MQ=255) gaTGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCcattcaacattcaacattcaacaaggaaagaaacttgtcgctggcatggacgtcaacattgatgatg > 2:709399‑M1/1‑82 (MQ=255) gAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCcattcaacattcaagattcaacaaggaaagaaacttgtcgctgacatggacgtcaacattgatgatgaga > 2:407482‑M1/1‑79 (MQ=255) gaggaTCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCcattcaacattcaacattcaacaaggaaagaaacttgtcgctggcatggacgtcaacattgatgatgagattggcg > 2:625642‑M1/1‑73 (MQ=255) gaggaTCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCcattcaacattcaacattcaacaaggaaagaaacttgtcgctggcatggacgtcaacattgatgatgagattggcg > 2:58700‑M1/1‑73 (MQ=255) aTACTATGAATGCATTTTTGCGTTTTCTGGATGCAGACATCCAGAACAACCCGCAAAAAACTCGTCcattcaacattcaacattcaacaaggaaagaaacttgtcgcttgcatggacgtcaacattgatgatgagattggcgacgacga < 1:368097‑M1/149‑84 (MQ=255) cTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCcattcaacattcaacattcaacaaggaaagaaacttgtcgctggcatggacgtcaacattgatgatgagattggcgacgacgaata < 1:625642‑M1/149‑87 (MQ=255) aTGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAACCTCGTCcattcaacattcaacattcaacaaggaaagaaacttgtcgctggcatggacgtcaacattgatgataagattgacgacgacaaataat > 2:249287‑M1/1‑61 (MQ=255) aTGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCcattcaacattcaacattcaacaaggaaagaaacttgtcgctggcatggacgtcaacattgatgatgaaattggcgacgacgaataat > 2:83280‑M1/1‑61 (MQ=255) aaTGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCcattcaacattcaacattcaacaaggaaagaaacttgtcgctggcatggacgtcaacattgatgatgagattggcgacgacgaataatgga < 2:313532‑M1/149‑92 (MQ=255) cATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCcattcaacattcaacattcaacaaggaaagaaacttgtcgctggcatggacgtcaacattgatgatgagattggcgacgacgaataatggatttt < 1:678976‑M1/149‑96 (MQ=255) cATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCcattcaacattcaacattcaacaaggaaagaaacttgtcgctggcatggacgtcaacattgatgatgagattggcgacgacgaataatggatttt < 2:774705‑M1/149‑96 (MQ=255) ttttGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAGCTCGTCcattcaacattcaacattcaacaaggaaagaaacttgtcgctgacatggacgacaacattgatgatgagattggcgccgacgaataatggactttcca > 1:406020‑M1/1‑51 (MQ=255) cTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCcattcaacattcaacattcaacaaggaaagaaacttgtcgctggcatggacgtcaacattgatgatgagattggcgacgacgaataatggattttccacaaagggttaa > 1:123539‑M1/1‑40 (MQ=255) agtgatatatagagaacaacccggaAAAGACTCGTCcattcaacattcaatattgagcaaggaaagaaactggtcgctggcatggacgtcaacattgaggatgagattggcgacgacgaataatggattttccacaaagggttaatggt < 1:664456‑M1/137‑114 (MQ=255) aagaagctatCCAGGACAACCCGCAAAAAACTCGTCCATTCAACATTCAACATTCACCAAGGAAGGAAACTTGTCGCTGGCCTGGACGTCAGCATTGATGATGGGGTTGGCCACGACGAATAAGGGATTGTCCGCAGAAGGATAATGGt > 1:133973/8‑149 (MQ=255) aTGCAGATATCCAGAACAACCCGCAAAAAACTCGACcattcaacattcaacattcaacagggaaagaaacttgtcgctggcatggacgtcaacattgatgatgagattggcgacgacgagtaatggattttccacaaagggttaatggt > 1:544306‑M1/1‑36 (MQ=255) gCAGATATCCAGAACAACCCGCAAAAAACTCGTCcattcaacattcaacattcaacaaggaaagaaacttgtcgctggcatggacgtcaacattgatgatgagattggcgacgacgaaaaatggattttccacaaagggttaatggttg > 2:28435‑M1/1‑34 (MQ=255) aGAACAACCCGCAAAAAACTCGTCcattcaacattcaacattcaacaaggaaagaaacttgtcgctggcatggacgtcaacattgatgatgagattggcgacgacgaataatggattttccacaaagggttaatggttgggcgctatat > 2:750408‑M1/1‑24 (MQ=255) acCCGCAAAAAACTCGTCcattcaacattcaacattcaacaaggaaagaaacttgtcgctggcatggacgtcaacattgatgatgagattggcgacgacgaataatggattttccacaaagggttaatggttgggcgctatatgctcat < 2:363452‑M1/149‑132 (MQ=255) aaaaaaCTCGTCcattcaacattcaacattcaacaaggaaagaaacttgtcgctggcatggacgtcaacattgatgatgagattggcgacgacgaataatggattttccacaaagggttaatggttgggcgctatatgctcatccctgt < 2:427881‑M1/149‑138 (MQ=255) aaaaaCTCGTCcattcaacattcaacattcaacaaggaaagaaacttgtcgctggcatggacgtcaacattgatgatgagattggcgacgacgaataatggattttccacaaagggttaatggttgggcgctatatgctcatccctgtt > 1:661444‑M1/1‑11 (MQ=255) aaaaaCTCGTCcattcaacattcaacattcaacaaggaaagaaacttgtcgctggcatggacgtcaacattgatgatgagagtggcgacgacgaataatggattttccacaaagggttaatggttgggcgctatatgctcatccctgtt < 2:380552‑M1/149‑139 (MQ=255) aCTCTTCcattcaacattcaacattcaacaaggaaagaaacttgtcgctggcatggacgtcaacattgatgatgagattggcgacgacgaataatggattttccacaaagggttaatggttgggcgctatatgctcatccctgttttca < 1:500335‑M1/149‑143 (MQ=255) cTCGTCcattcaacattcaacattcaacaaggaaagaaacttgtcgctggcatggacgtcaacattgatgatgagattggcgacgacgaataatggattttccacaaagggttaatggttgggcgctatatgctcatccctgttttcag < 1:524387‑M1/149‑144 (MQ=255) tCGTCcattcaacattcaacattaaagaatgaaagaaacttgccgctggcatggacgtcaacattgaggatgagattggcgacgacgaataatggattttccacaaagggttaatggttgggcgctatatgctcatccctgttttcagg < 2:257343‑M1/149‑145 (MQ=255) tCcattcaacattcaacattcaacaaggaaagaaacttgtcgctggcatggacgtcaacattgatgatgagattggcgacgacgaataatggattttccacaaagggttaatggttgggcgctatatgctcatccctgttttcaggaaa < 2:646004‑M1/149‑148 (MQ=255) | AGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTATGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCCATTCAACATTCAA‑‑‑‑‑‑‑CAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAA > CP009273/3270476‑3270756 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
Reads not counted as support for junction |
read_name Not counted due to insufficient overlap past the breakpoint. |
read_name Not counted due to not crossing MOB target site duplication. |