Predicted mutation
evidence seq id position mutation annotation gene description
JC CP009273 3,270,632 (TTCAACA)2→3 coding (272/336 nt) prlF → antitoxin of the SohA(PrlF)‑YhaV toxin‑antitoxin system

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009273 = 32706161 (0.020)38 (0.850)
+CATTCAA
35/250 0.2 97.4% coding (256/336 nt) prlF antitoxin of the SohA(PrlF)‑YhaV toxin‑antitoxin system
?CP009273 3270617 = 1 (0.020)coding (257/336 nt) prlF antitoxin of the SohA(PrlF)‑YhaV toxin‑antitoxin system

CCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTATGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCCATTCAACATTCAA‑‑‑‑‑‑‑CAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTAC  >  CP009273/3270469‑3270761
                                                                                                                                                    |                                                                                                                                                       
aggacagcattcattacgaaattctgcctggtgggcaagtatttatgtgccgactgggagatgaacaggaggatcatactatgaatgcatttttgcgttttctggatgcagatatccagaacaacccgcaaaaaactctcccactcaac                                                                                                                                                         >  1:305024‑M2/149‑149 (MQ=255)
   acagcattcattacgaaattctgcctggtgggcaagtatttatgtgccgactgggagatgaacaggaggatcatactatgaatgcatttttgcgttttctggatgcagatatccagaacaacccgcaaaaaactcgtccattcaacatt                                                                                                                                                      <  2:749331‑M2/4‑1 (MQ=255)
     agcattcattacgaaattctgcctggtgggcaagtatttatgtgccgactgggagatgaacaggaggatcatactatgaatgcatttttgcgttttctggatgcagatatccagaacaacccgcaaaaaactcgtccattcaacattca                                                                                                                                                    <  2:261600‑M2/6‑1 (MQ=255)
        attcattacgaaattctgcctggtgggcaagtatttatgtgccgactgggagatgaacaggaggatcatactatgaatgcatttttgcgttttctggatgcagatatccagaacaacccgcaaaaaactcgtccattcaaaattcaaca                                                                                                                                                 >  2:594273‑M2/141‑149 (MQ=255)
                 gaaattctgcctggtgggcaagtatttatgtgccgactgggagatgaacaggaggatcatactatgaatgcatttttgcgttttctggatgcagatatccagaacaacccgcaaaaaactcgtccattcaaCAGTCAACATTCAA‑‑‑‑‑‑‑CAAg                                                                                                                                 >  1:723984‑M2/132‑149 (MQ=255)
                 gaaattctgcctggtgggcaagtatttatgtgccgactgggagatgaacaggaggatcatactatgaatgcatttttgcgttttctggatgcagatatccagaaaaacccgcaaaaaactcgtccagtccaCGTACAACATTCAA‑‑‑‑‑‑‑CAAg                                                                                                                                 >  1:313532‑M2/132‑149 (MQ=255)
                   aattctgcctggtgggcaagtatttatgtgccgactgggagatgaacaggaggatcatactatgaatgcatttttgcgttttctggatgcagatatccagaacaacccgcaaaaaactcgtccgttcaaCATTCAACATTAAA‑‑‑‑‑‑‑CAAGga                                                                                                                               >  1:212284‑M2/130‑149 (MQ=255)
                   aattctgcctgctgggcagggatttatgtgccgactgggagatgaacaggaggatcatactatgaatgcatttttgcgttttctggatgcagatatccagaacaacccgcaaaaaactcgtccattcaaCATTCAACATTCAA‑‑‑‑‑‑‑CAAGga                                                                                                                               <  2:67939‑M2/20‑1 (MQ=255)
                    attctgcctggtgggcaagtatttatgtgccgactgggagatgaacaggaggatcatactatgaatgcatttttgcgttttctggatgcagatatccagaacaacccgcaaaaaactcgtccattcaaCATTCAACATTCAA‑‑‑‑‑‑‑CAAGgaa                                                                                                                              >  2:500335‑M2/129‑149 (MQ=255)
                       ctgcctggtgggcaagtatttatgtgaccactggaagacgaacaagaagatcatattatgaatgcatttttgcgttttctggatgcagatatccagaacaacccgcaaaaaactcgtccattcaaCATTCAACATTCAA‑‑‑‑‑‑‑CAAGgaaaga                                                                                                                           <  2:335299‑M2/24‑1 (MQ=255)
                        tgcctggtgggcaagtatttatgtgccgactgggggatgaacaggaggatcatactatgaatgcctttttgcgttttctggatgcagatatccagaacaccccgcaaaaaactcggccattcaaCATTCAACATTGCA‑‑‑‑‑‑‑CAAGgaaagaa                                                                                                                          >  2:145660‑M2/125‑149 (MQ=255)
                           ctggtgggcaagtatttatgtgccgactgggagatgaacaggaggatcatactatgaatgcatttttgcgttttctggatgcagatatccagaacaacccgcaaaaaactcgtccattcaaCATTCAACATTCAA‑‑‑‑‑‑‑CAAGGAAAGAAACt                                                                                                                       >  1:774705‑M2/122‑149 (MQ=255)
                            tggtgggcaagtatttatgtgccgactgggagatgaacaggaggatcatactatgaatgcatttttgcgttttctggatgcagatatccagaacaacccgcaaaaaactcgtccattcaaCATTCAACATTCAA‑‑‑‑‑‑‑CAAGGAAAGAAACtt                                                                                                                      <  2:673857‑M2/29‑1 (MQ=255)
                                   caagtatttatgtgccgactgggagatgaacaggaggatcatactatgaatgcatttttgcgttttctggatgcagatatccagaacaacccgcaaaaaactcgtccattcaaCATTCAACATTCAA‑‑‑‑‑‑‑CAAGGAAAGAAACTTGTCGCTg                                                                                                               >  2:391767‑M2/114‑149 (MQ=255)
                                             tgtgccgactgggagatgaacaggaggatcatactatgaatgcatttttgcgttttctggatgcagatatccagaacaacccgcaaaaaactcgtccattcaaCATTCAACATTCAC‑‑‑‑‑‑‑CAAGGAAAGAAACTTGTCGCTGGCATGGACGt                                                                                                     >  2:190418‑M2/104‑149 (MQ=255)
                                             tgtgccgactgggagatgaacaggaggatcatactatgaatgcatttttgcgttttctggatgcagatatccagaacaacccgcaaaaaactcgtccaaccaaCATTCAACAATAAA‑‑‑‑‑‑‑CAAAGAAAGAAAATAATAACTAGCATGGACGt                                                                                                     >  2:162874‑M2/104‑149 (MQ=255)
                                                gccgactgggagatgaacaggaggatcatactatgaatgcatttttgcgttttctggatgcagatatccagaacaacccgcaaaaaactcgtccattcaaCATTCAACATTCAA‑‑‑‑‑‑‑CAAGGAAAGAAACTTGTCGCTGGCATGGACGTCaa                                                                                                  >  1:537662‑M2/101‑149 (MQ=255)
                                                  cgtctgggagatgaacagggggatcatactatgaatgcatttttgcgttttctggatgcagatatccagaacaacccgcaaaaaactcgtccattcaaCATTCAACATTCAA‑‑‑‑‑‑‑CAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACa                                                                                                <  1:148678‑M2/51‑1 (MQ=255)
                                                       cgtagatgaacaacaggatcatactaacaatgcatttttccattttctggatgcagatatccagaacaacccgcaaaaaaatcgtccattcaaCATTCAACATTCAA‑‑‑‑‑‑‑CAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATtgat                                                                                           <  2:66077‑M2/56‑1 (MQ=255)
                                                          agatgaacaggaggatcatactatgaatgcatttttgcgttttctggatgcagatatccagaacaacccgcaaaaaactcgtccattcaaCATTCAACATTCAA‑‑‑‑‑‑‑CAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACCTtgatgat                                                                                        >  2:307361‑M2/91‑149 (MQ=255)
                                                           gatgaacaggaggatcatactatgaatgcatttttgcgttttctggatgcagatatccagaacaacccgcaaaaaactcgtccattcaaCATTCAACATTCAA‑‑‑‑‑‑‑CAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATtgatgatg                                                                                       >  2:709399‑M2/90‑149 (MQ=255)
                                                              gaacaggaggatcatactatgaatgcatttttgcgttttctggatgcagatatccagaacaacccgcaaaaaactcgtccattcaaCATTCAAGATTCAA‑‑‑‑‑‑‑CAAGGAAAGAAACTTGTCGCTGACATGGACGTCAACATTGATGATgaga                                                                                    >  2:407482‑M2/87‑149 (MQ=255)
                                                                    gaggatcatactatgaatgcatttttgcgttttctggatgcagatatccagaacaacccgcaaaaaactcgtccattcaaCATTCAACATTCAA‑‑‑‑‑‑‑CAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGcg                                                                              >  2:58700‑M2/81‑149 (MQ=255)
                                                                    gaggatcatactatgaatgcatttttgcgttttctggatgcagatatccagaacaacccgcaaaaaactcgtccattcaaCATTCAACATTCAA‑‑‑‑‑‑‑CAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGcg                                                                              >  2:625642‑M2/81‑149 (MQ=255)
                                                                           atactatgaatgcatttttgcgttttctggatgcagacatccagaacaacccgcaaaaaactcgtccattcaaCATTCAACATTCAA‑‑‑‑‑‑‑CAAGGAAAGAAACTTGTCGCTTGCATGGACGTCAACATTGATGATGAGATTGGcgacgacga                                                                       <  1:368097‑M2/76‑1 (MQ=255)
                                                                              ctatgaatgcatttttgcgttttctggatgcagatatccagaacaacccgcaaaaaactcgtccattcaaCATTCAACATTCAA‑‑‑‑‑‑‑CAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGaata                                                                    <  1:625642‑M2/79‑1 (MQ=255)
                                                                                atgaatgcatttttgcgttttctggatgcagatatccagaacaacccgcaaaaacctcgtccattcaaCATTCAACATTCAA‑‑‑‑‑‑‑CAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATAAGATTGACGACGACAaataat                                                                  >  2:249287‑M2/69‑149 (MQ=255)
                                                                                atgaatgcatttttgcgttttctggatgcagatatccagaacaacccgcaaaaaactcgtccattcaaCATTCAACATTCAA‑‑‑‑‑‑‑CAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAAATTGGCGACGACGaataat                                                                  >  2:83280‑M2/69‑149 (MQ=255)
                                                                                   aatgcatttttgcgttttctggatgcagatatccagaacaacccgcaaaaaactcgtccattcaaCATTCAACATTCAA‑‑‑‑‑‑‑CAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGa                                                               <  2:313532‑M2/84‑1 (MQ=255)
                                                                                       catttttgcgttttctggatgcagatatccagaacaacccgcaaaaaactcgtccattcaaCATTCAACATTCAA‑‑‑‑‑‑‑CAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGAtttt                                                           <  1:678976‑M2/88‑1 (MQ=255)
                                                                                       catttttgcgttttctggatgcagatatccagaacaacccgcaaaaaactcgtccattcaaCATTCAACATTCAA‑‑‑‑‑‑‑CAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGAtttt                                                           <  2:774705‑M2/88‑1 (MQ=255)
                                                                                          ttttgcgttttctggatgcagatatccagaacaacccgcaaaaagctcgtccattcaaCATTCAACATTCAA‑‑‑‑‑‑‑CAAGGAAAGAAACTTGTCGCTGACATGGACGACAACATTGATGATGAGATTGGCGCCGACGAATAATGGACTTTCca                                                        >  1:406020‑M2/59‑149 (MQ=255)
                                                                                                     ctggatgcagatatccagaacaacccgcaaaaaactcgtccattcaaCATTCAACATTCAA‑‑‑‑‑‑‑CAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGGTTaa                                             >  1:123539‑M2/48‑149 (MQ=255)
                                                                                                         atgcagatatccagaacaacccgcaaaaaactcgaccattcaaCATTCAACATTCAA‑‑‑‑‑‑‑CAGGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAGTAATGGATTTTCCACAAAGGGTTAATGGt                                         >  1:544306‑M2/44‑149 (MQ=255)
                                                                                                         agtgatatatagagaacaacccggaaaagactcgtccattcaaCATTCAATATTGAG‑‑‑‑‑‑‑CAAGGAAAGAAACTGGTCGCTGGCATGGACGTCAACATTGAGGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAATGGt                                         <  1:664456‑M2/106‑1 (MQ=255)
                                                                                                           gcagatatccagaacaacccgcaaaaaactcgtccattcaaCATTCAACATTCAA‑‑‑‑‑‑‑CAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAAAAATGGATTTTCCACAAAGGGTTAATGGTTg                                       >  2:28435‑M2/42‑149 (MQ=255)
                                                                                                                aagaagctatCCAGGACAACCCGCAAAAAACTCGTCCATTCAACATTCAACATTCACCAAGGAAGGAAACTTGTCGCTGGCCTGGACGTCAGCATTGATGATGGGGTTGGCCACGACGAATAAGGGATTGTCCGCAGAAGGATAATGGt                                         >  1:133973/8‑149 (MQ=255)
                                                                                                                     agaacaacccgcaaaaaactcgtccattcaaCATTCAACATTCAA‑‑‑‑‑‑‑CAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTatat                             >  2:750408‑M2/32‑149 (MQ=255)
                                                                                                                           acccgcaaaaaactcgtccattcaaCATTCAACATTCAA‑‑‑‑‑‑‑CAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCAt                       <  2:363452‑M2/124‑1 (MQ=255)
                                                                                                                                 aaaaaactcgtccattcaaCATTCAACATTCAA‑‑‑‑‑‑‑CAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGt                 <  2:427881‑M2/130‑1 (MQ=255)
                                                                                                                                  aaaaactcgtccattcaaCATTCAACATTCAA‑‑‑‑‑‑‑CAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGtt                >  1:661444‑M2/19‑149 (MQ=255)
                                                                                                                                  aaaaactcgtccattcaaCATTCAACATTCAA‑‑‑‑‑‑‑CAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAGTGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGtt                <  2:380552‑M2/131‑1 (MQ=255)
                                                                                                                                      actcttccattcaaCATTCAACATTCAA‑‑‑‑‑‑‑CAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCa            <  1:500335‑M2/135‑1 (MQ=255)
                                                                                                                                       ctcgtccattcaaCATTCAACATTCAA‑‑‑‑‑‑‑CAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAg           <  1:524387‑M2/136‑1 (MQ=255)
                                                                                                                                        tcgtccattcaaCATTCAACATTAAA‑‑‑‑‑‑‑GAATGAAAGAAACTTGCCGCTGGCATGGACGTCAACATTGAGGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAgg          <  2:257343‑M2/137‑1 (MQ=255)
                                                                                                                                           tccattcaaCATTCAACATTCAA‑‑‑‑‑‑‑CAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGaaa       <  2:646004‑M2/140‑1 (MQ=255)
                                                                                                                                             cattcaaCATTCAACATTCAA‑‑‑‑‑‑‑CAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGGGATTGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAAcc     >  2:88385‑M2/8‑149 (MQ=255)
                                                                                                                                                tcaaCATTCAACATTCAA‑‑‑‑‑‑‑CAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTac  >  2:75555‑M2/5‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                       
CCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTATGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCCATTCAACATTCAA‑‑‑‑‑‑‑CAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTAC  >  CP009273/3270469‑3270761

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.