New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP000730 1410425 =29 (1.000)3 (0.110) 3/246 3.7 10.2% coding (483/729 nt) trpA tryptophan synthase alpha subunit
?CP000730 1410516 = 26 (0.960)coding (574/729 nt) trpA tryptophan synthase alpha subunit
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

ACTGGCACATTGGCTATCGCTTTAATTGACTCAATTTTTCGTTTTAATTCTGGATGAAACGCACCGTTTTGCCCTGTTGTCGCA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  CP000730/1410508‑1410425
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gttgtcgcaGGATTTGGTATAAGAACACCACAACATGTTGCAGATATAAAAGAGGTTGCAGATGGCATTG  >  CP000730/1410516‑1410576
                                                                                                                                                 
ACTGGCACATTGGCTATCGCTTTAATTGACTCAATTTTTCGTTTTAATTCTGGATGAAACGCACCGTTTTGCCCTGTTGTCGCAGGATTTGGTATAAGAACACCACAACATGTTGCAGATATAAAAGAGGTTGCAGATGGC      >  1:302147/1‑141
 CTGGCACATTGGCTATCGCTTTAATTGACTCAATTTTTCGTTTTAATTCTGGATGAAACGCACCGTTTTGCCCTGTTGTCGCAGGATTTGGTATAAGAACACCACAACATGTTGCAGATATAAAAGAGGTTGCAGATGGCA     >  1:297231/1‑141
    GCACATTGGCTATCGCTTTAATTGACTCAATTTTTCGTTTTAATTCTGGATGAAACGCACCGTTTTGCCCTGTTGTCGCAGGATTTGGTATAAGAACACCACAACATGTTGCAGATATAAAAGAGGTTGCAGATGGCGTTG  <  2:297231/141‑1
                                                                                                                                                 
ACTGGCACATTGGCTATCGCTTTAATTGACTCAATTTTTCGTTTTAATTCTGGATGAAACGCACCGTTTTGCCCTGTTGTCGCA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  CP000730/1410508‑1410425
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gttgtcgcaGGATTTGGTATAAGAACACCACAACATGTTGCAGATATAAAAGAGGTTGCAGATGGCATTG  >  CP000730/1410516‑1410576

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.