New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	CP000730	1410425 =	29 (1.000)	3 (0.110)	3/246	3.7	10.2%	coding (483/729 nt)	trpA	tryptophan synthase alpha subunit
	?	CP000730	1410516 =	26 (0.960)					coding (574/729 nt)	trpA	tryptophan synthase alpha subunit
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TTATCGCAGCGTTTAAAACAACAATTTAGTCACTATGGCGTCAAAATCATATCGTTAGTTGCGATGACTACTGATGACAAACGTATAAAAGATATCGTATCCCATGCGGAAGGCTTTATTTATACTGTGACGATGAATGCGACAACAGGGCAAAACGGTGCGTTTCATCCAGAATTAAAACGAAAAATTGAGTCAATTAAAGCGATAGCCAATGTGCCAGTTGTCGCAGGATTTGGTATAAGAACACCACAACATGTTGCAGATATAAAAGAGGT  >  CP000730/1410288‑1410562                                                                                                                                          |                                                                                                                                          ttATCGCAGCGTTTAAAACAACAATTTAGTCACTATGGCGTCAAAATCATATCGTTAGTTGCGATGACTACTGATGACAAACGTATAAAAGATATCGTATCCCATGCGGAAGGCTTTATTTATACTGTGACGATGAATGCg                                                                                                                                        >  1:80524/1‑141 (MQ=255)                     acaaTTTAGTCACTATGGCGTCAAAATCATATCGTTAGTTGCGATGACTACTGATGACAAACGTATAAAAGATATCGTATCCCATGCGGAAGGCTTTATTTATACTGTGACGATGAATGCGACAACAGGGCAAAACGGTGc                                                                                                                    >  2:38218/1‑141 (MQ=255)                      caaTTTAGTCACTATGGCGTCAAAATCATATCGTTAGTTGCGATGAATAATGATGACAAACGTATAAAACATATCGTACCCCATACCGAAGTTTTTATTTCTACTGTGACGATTAATGCGAAAACAGGGCAAAACGGTGCg                                                                                                                   <  2:241322/141‑1 (MQ=255)                         tttAGTCACTATGGCGTCAAAATCATATCGTTAGTTGCGATGACTACTGATGACAAACGTATAAAAGATATCGTATCCCATGCGGAAGGCTTTATTTATACTGTGACGATGAATGCGa                                                                                                                                       >  1:307255/1‑118 (MQ=255)                         tttAGTCACTATGGCGTCAAAATCATATCGTTAGTTGCGATGACTACTGATGACAAACGTATAAAAGATATCGTATCCCATGCGGAAGGCTTTATTTATACTGTGACGATGAATGCGa                                                                                                                                       <  2:307255/118‑1 (MQ=255)                            aGTCACTATGGCGTCAAAATCATATCGTTAGTTGCGATGACTACTGATGACAAACGTATAAAAGATATCGTATCCCATGCGGAAGGCTTTATTTATACTGTGACGATGAATGCGACAACAGGGCAAAACGGTGCGTTTCAt                                                                                                             >  1:139179/1‑141 (MQ=255)                             gTCACTATGGCGACAAAATCTGATCGTTAGTTGCGATGACTACTGATGACAAACGTATAAAAGATATCGTATCCCATGCGGAAGGCTTTATTTATACTGTGACGATGAATGCGACAACAGGGCAAAACGGTGCGTTTCATc                                                                                                            <  1:272181/141‑1 (MQ=255)                             gTCAATATGGCGTCAAAATCATATCGTTAGTTGCGATGACTACTGATGACAAACGTATAAAAGATATCGTATCCCATGCGGAAGGCTTTATTTATACTGTGACGATGAATGCGACAACAGGGCAAAACGGTGCGTTTCATc                                                                                                            <  1:272198/141‑1 (MQ=255)                                  tATGGCGTCAAAATCATATCGTTAGTTGCGATGACTACTGATGACAAACGTATAAAAGATATCGTATCCCATGCGGAAGGCTTTATTTATACTGTGACGATGAATGCGACAACAGGGCAAAACGGTGCGTTTCATCCAGaa                                                                                                       >  2:192818/1‑141 (MQ=255)                                       cGTCAAAATCATATCGTTAGTTGCGATGACTACTGATGACAAACGTATAAAAGATATCGTATCCCATGCGGAAGGCTTTATTTATACTGTGACGATGAATGCGACAACAGGGCAAAACGGTGCGTTTCATCCAGAATTaaa                                                                                                  >  1:282419/1‑141 (MQ=255)                                         tCAAAATCATATCGTTAGTTGCGATGACTACTGATGACAAACGTATAAAAGATATCGTATCCCATGCGGAAGGCTTTATTTATACTGTGACGATGAATGCGACAACAGGGCAAAACGGTGCGTTTCATCCAGAATTAAAAc                                                                                                >  2:225860/1‑141 (MQ=255)                                                 atatCGTTAGTTGCGATGACTACTGATGACAAACGTATAAAAGATATCGTATCCCATGCGGAAGGCTTTATTTATACTGTGACGATGAATGCGACAACAGGGCAAAACGGTGCGTTTCATCCAGAATTAAAACGAAAAAtt                                                                                        <  1:105558/141‑1 (MQ=255)                                                    tCGTTAGTTGCGATGACTACTGATGACAAACGTATAAAAGATATCGTATCCCATGCGGAAGGCTTTATTTATACTGTGACGATGAATGCGACAACAGGGCAAAACGGTGCGTTTCATCCAGAATTaaaa                                                                                                 <  2:216615/129‑1 (MQ=255)                                                    tCGTTAGTTGCGATGACTACTGATGACAAACGTATAAAAGATATCGTATCCCATGCGGAAGGCTTTATTTATACTGTGACGATGAATGCGACAACAGGGCAAAACGGTGCGTTTCATCCAGAATTaaaa                                                                                                 >  1:216615/1‑129 (MQ=255)                                                             gCGATGACTACTGATGACAAACGTATAAAAGATATCGTATCCCATGCGGAAGGCTTTATTTATACTGTGACGATGAATGCGACAACAGGGCAAAACGGTGCGTTTCATCCAGAATAAAAAAAAAAAATTGAGTCAATTaaa                                                                            <  2:108363/141‑1 (MQ=255)                                                                      actGATGACAAACGTATAAAAGATATCGTATCCCATGCGGAAGGCTTTATTTATACTGTGACGATGAATGCGACAACAGGGCAAAACGGTGCGTTTCATCCAGAATTAAAACGAAAAATTGAGTCAATTAAAGCGATAGcc                                                                   <  1:38218/141‑1 (MQ=255)                                                                                      taAAAGATATCGTATCCCATGCGGAAGGCTTTATTTATACTGTGACGATGAATGCGACAACAGGGCAAAACGGTGCGTTTCATCCAGAATTAAAACGAAAAATTGAGTCAATTAAAGCGATAGCCAATGTGCCAGTTGTCg                                                   <  2:177018/141‑1 (MQ=255)                                                                                         aaGATATCGTATCCCATGCGGAAGGCTTTATTTATACTGTGACGATGAATGCGACAACAGGGCAAAACGGTGCGTTTCATCCAGAATTAAAACGAAAAATTGAGTCAATTAAAGCGATAGCCAATGTGCCAGTTGTCGCAg                                                <  2:40097/141‑1 (MQ=255)                                                                                         aaGATATCGTATCCCATGCGGAAGGCTTTATTTATACTGTGACGATGAATGCGACAACAGGGCAAAACGGTGCGTTTCATCCAGAATTAAAACGAAAAATTGAGTCAATTAAAGCGATAGCCAATGTGCCAGTTGTCGCAg                                                <  1:233930/141‑1 (MQ=255)                                                                                         aaGATATCGTATCCCATGCGGAAGGATTTATTTATACTGTGACGATGAATGCGAAAACAGGGCAAAACGGTGCGTTTCATCAAAAAAAAAAAAGAAAAATTGAGTCAATTAAAGCGATAGCCAATGTGCCAGTTGTCGCAg                                                <  2:126420/141‑1 (MQ=255)                                                                                                    tCCCATGCGGAAGGCTTTATTTATACTGTGACGATGAATGCGACAACAGGGCAAAACGGTGCGTTTCATCCAGAATTAAAACGAAAAATTGAGTCAATTAAAGCGATAGCCAATGTGCCAGTTGTCGCAGGATTTGGtata                                     <  1:291546/141‑1 (MQ=255)                                                                                                    tCCCATGCGGAAGGCTTTATTTATACTGTGACGATGAATGCGACAACAGGGCAAAACGGTGCGTTTCATCCAGAATTAAAACGAAAAATTGAGTCAATTAAAGCGATAGCCAATGTGCCAGTTGTCGCAGGATTTGGtata                                     <  1:291571/141‑1 (MQ=255)                                                                                                        aTGCGGAAGGCTTTATTTATACTGTGACGATGAATGCg                                                                                                                                        >  1:305532/1‑38 (MQ=255)                                                                                                        aTGCGGAAGGCTTTATTTATACTGTGACGATGAATGCg                                                                                                                                        <  2:305532/38‑1 (MQ=255)                                                                                                               aGGCTTTATTTATACTGTGACGATGAATGCGACAACAGGGCAAAACGGTGCGTTTCATCCAGAATTAAAACGAAAAATTGAGTCAATTAAAGCGATAGCCAATGTGCCAGTTGTCGCAGGATTTGGTATAAGAACACcaca                          >  1:194102/1‑141 (MQ=255)                                                                                                               aGGCTTTATTTATACTGTGACGATGAATGCGACAACAGGGCAAAACGGTGCGTTTCATCCAGAATTAAAACGAAAAATTGAGTCAATTAAAGCGATAGCCAATGTGCCAGTTGTCGCAGGATTTGGTATAAGAACACcaca                          >  1:38410/1‑141 (MQ=255)                                                                                                                ggCTTTATTTATACTGTGACGATGAATGCGACAACAGGGCAAAACGGTGCGTTTCATCCAGAATTAAAACGAAAAATTGAGTCAATTAAAGCGATAGCCAATGTGCCAGTTGTCGCAGGATTTGGTATAAGAACACCacaa                         <  2:319647/141‑1 (MQ=255)                                                                                                                     tatttatACTGTGACGATGAATGCGACAACAGGGCAAAACGGTGCGTTTCATCCAGAATTAAAACGAAAAATTGAGTCAATTAAAGCGATAGCCAATGTGCCAGTTGTCGCAGGATTTGGTATAAGAACACCACAACATGt                    >  1:324082/1‑141 (MQ=255)                                                                                                                                       gAATGCGACAACAGGGCAAAACGGTGCGTTTCATCCAGAATTAAAACGAAAAATTGAGTCAATTAAAGCGATAGCCAATGTGCCAGTTGTCGCAGGATTTGGTATAAGAACACCACAACATGTTGCAgtggcctgttaaga  >  2:325281/1‑128 (MQ=255)                                                                                                                                          |                                                                                                                                          TTATCGCAGCGTTTAAAACAACAATTTAGTCACTATGGCGTCAAAATCATATCGTTAGTTGCGATGACTACTGATGACAAACGTATAAAAGATATCGTATCCCATGCGGAAGGCTTTATTTATACTGTGACGATGAATGCGACAACAGGGCAAAACGGTGCGTTTCATCCAGAATTAAAACGAAAAATTGAGTCAATTAAAGCGATAGCCAATGTGCCAGTTGTCGCAGGATTTGGTATAAGAACACCACAACATGTTGCAGATATAAAAGAGGT  >  CP000730/1410288‑1410562

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.