New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP000730 1410425 =29 (1.000)3 (0.110) 3/246 3.7 10.2% coding (483/729 nt) trpA tryptophan synthase alpha subunit
?CP000730 1410516 = 26 (0.960)coding (574/729 nt) trpA tryptophan synthase alpha subunit
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

AAGATATCGTATCCCATGCGGAAGGCTTTATTTATACTGTGACGATGAATGCGACAACAGGGCAAAACGGTGCGTTTCATCCAGAATTAAAACGAAAAATTGAGTCAATTAAAGCGATAGCCAATGTGCCAGTTGTCGCAGGATTTGGTATAAGAACACCACAACATGTTGCAGATATAAAAGAGGTTGCAGATGGCATTGTCATTGGTAGCGAAATCGTTAAGCGATTTAAATCTAACACGCGTGAGGAAATCATTAAATATTTACAATCTATCCAACAA  >  CP000730/1410376‑1410656
                                                                                                                                            |                                                                                                                                            
aaGATATCGTATCCCATGCGGAAGGCTTTATTTATACTGTGACGATGAATGCGACAACAGGGCAAAACGGTGCGTTTCATCCAGAATTAAAACGAAAAATTGAGTCAATTAAAGCGATAGCCAATGTGCCAGTTGTCGCAg                                                                                                                                              <  1:233930/141‑1 (MQ=255)
aaGATATCGTATCCCATGCGGAAGGCTTTATTTATACTGTGACGATGAATGCGACAACAGGGCAAAACGGTGCGTTTCATCCAGAATTAAAACGAAAAATTGAGTCAATTAAAGCGATAGCCAATGTGCCAGTTGTCGCAg                                                                                                                                              <  2:40097/141‑1 (MQ=255)
aaGATATCGTATCCCATGCGGAAGGATTTATTTATACTGTGACGATGAATGCGAAAACAGGGCAAAACGGTGCGTTTCATCAAAAAAAAAAAAGAAAAATTGAGTCAATTAAAGCGATAGCCAATGTGCCAGTTGTCGCAg                                                                                                                                              <  2:126420/141‑1 (MQ=255)
           tCCCATGCGGAAGGCTTTATTTATACTGTGACGATGAATGCGACAACAGGGCAAAACGGTGCGTTTCATCCAGAATTAAAACGAAAAATTGAGTCAATTAAAGCGATAGCCAATGTGCCAGTTGTCGCAGGATTTGGtata                                                                                                                                   <  1:291571/141‑1 (MQ=255)
           tCCCATGCGGAAGGCTTTATTTATACTGTGACGATGAATGCGACAACAGGGCAAAACGGTGCGTTTCATCCAGAATTAAAACGAAAAATTGAGTCAATTAAAGCGATAGCCAATGTGCCAGTTGTCGCAGGATTTGGtata                                                                                                                                   <  1:291546/141‑1 (MQ=255)
                      aGGCTTTATTTATACTGTGACGATGAATGCGACAACAGGGCAAAACGGTGCGTTTCATCCAGAATTAAAACGAAAAATTGAGTCAATTAAAGCGATAGCCAATGTGCCAGTTGTCGCAGGATTTGGTATAAGAACACcaca                                                                                                                        >  1:38410/1‑141 (MQ=255)
                      aGGCTTTATTTATACTGTGACGATGAATGCGACAACAGGGCAAAACGGTGCGTTTCATCCAGAATTAAAACGAAAAATTGAGTCAATTAAAGCGATAGCCAATGTGCCAGTTGTCGCAGGATTTGGTATAAGAACACcaca                                                                                                                        >  1:194102/1‑141 (MQ=255)
                       ggCTTTATTTATACTGTGACGATGAATGCGACAACAGGGCAAAACGGTGCGTTTCATCCAGAATTAAAACGAAAAATTGAGTCAATTAAAGCGATAGCCAATGTGCCAGTTGTCGCAGGATTTGGTATAAGAACACCacaa                                                                                                                       <  2:319647/141‑1 (MQ=255)
                            tatttatACTGTGACGATGAATGCGACAACAGGGCAAAACGGTGCGTTTCATCCAGAATTAAAACGAAAAATTGAGTCAATTAAAGCGATAGCCAATGTGCCAGTTGTCGCAGGATTTGGTATAAGAACACCACAACATGt                                                                                                                  >  1:324082/1‑141 (MQ=255)
                                              gAATGCGACAACAGGGCAAAACGGTGCGTTTCATCCAGAATTAAAACGAAAAATTGAGTCAATTAAAGCGATAGCCAATGTGCCAGTTGTCGCAGGATTTGGTATAAGAACACCACAACATGTTGCAgtggcctgttaaga                                                                                                >  2:325281/1‑128 (MQ=255)
                                                             gCAAAACGGTGCGTTTCATCCAGAATTAAAACGAAAAATTGAGTCAATTAAAGCGATAGCCAATGTGCCAGTTGTCGCAGGATTTGGTATAAGAACACCACAACATGTTGCAGATATAAAAGAGGTTGCAGATGGCATTGt                                                                                 >  1:28933/1‑141 (MQ=255)
                                                              cAAAACGGTGCGTTTCATCCAGAATTAAAACGAAAAATTGAGTCAATTAAAGCGATAGCCAATGTGCCAGTTGTCGCAGGATTTGGTATAAGAACACCACAACATGTTGCAGATATAAAAGAGGTTGCAGATGGCATTGTc                                                                                <  1:106732/141‑1 (MQ=255)
                                                                        cGTTTCATCCAGAATTAAAACGAAAAATTGAGTCAATTAAAGCGATAGCCAATGTGCCAGTTGTCGCAGGATTTGGTATAAGAACACCACAACATGTTGCAGATATAAAAGAGGTTGCAGATGGCATTGTCATTGGTAGCg                                                                      >  2:288927/1‑141 (MQ=255)
                                                                                   gAATTAAAACGAAAAATTGAGTCAATTAAAGCGATAGCCAATGTGCCAGTTGTCGCAGGATTTGGTATAAGAACACCACAACATGTTGCAGATATAAAAGAGGTTGCAGATGGCATTGTCATTGGTAGCGAAATCGTTAAg                                                           <  2:80524/141‑1 (MQ=255)
                                                                                   gAATTAAAACGAAAAATTGAGTCAATTAAAGCGATAGCCAATGTGCCAGTTGTCGCAGGATTTGGTATAAGAACACCAAAACATGTTGCAGATATAAAAGAGGTTGCAGATGTCATTGTCATTGGTAGCGAAATCGTTAAg                                                           <  2:139179/141‑1 (MQ=255)
                                                                                         aaaCGAAAAATTGAGTCAATTAAAGCGATAGCCAATGTGCCAGTTGTCGCAGGATTTGGTATAAGAACACCACAACATGTTGCAGATATAAAAGAGGTTGCAGATGGCATTGTCATTGGTAGCGAAATCGTTAAGCGAttt                                                     >  2:168542/1‑141 (MQ=255)
                                                                                                  aTTGAGTCAATTAAAGCGATAGCCAATGTGCCAGTTGTCGCAGGATTTGGTATAAGAACACCACAACATGTTGCAGATATAAAAGAGGTTGCAGATGGCATTGTCATTGGTAGCGAAATCGTTAAGCGATTTAAATCTAac                                            >  2:121311/1‑141 (MQ=255)
                                                                                                  aTTGAGTCAATTAAAGCGATAGCCAATGTGCAAGTTGTCGCAGGATTTGGTATAAGAACACCACAACATGTTGCAGATATAAAAGAGGTTGCAGATGGCATTGTCATTGGTAGCGAAATCGTTAAGCGATTTAAATCTAac                                            >  1:25827/1‑141 (MQ=255)
                                                                                                     gAGTCAATTAAAGCGATAGCCAATGTGCCAGTTGTCGCAGGATTTGGta                                                                                                                                     >  2:145785/1‑49 (MQ=255)
                                                                                                     gAGTCAATTAAAGCGATAGCCAATGTGCCAGTTGTCGCAGGATTTGGta                                                                                                                                     <  1:145785/49‑1 (MQ=255)
                                                                                                            ttAAAGCGATAGCCAATGTGCCAGTTGTCGCAGGATTTGGTATAAGAACACCACAACATGTTGCAGATATAAAagag                                                                                                  >  2:261572/1‑77 (MQ=255)
                                                                                                            ttAAAGCGATAGCCAATGTGCCAGTTGTCGCAGGATTTGGTATAAGAACACCACAACATGTTGCAGATATAAAagag                                                                                                  <  1:261572/77‑1 (MQ=255)
                                                                                                                   gATAGCCAATGTGCCAGTTGTCGCAGGATTTGGTATAAGAACACCACAACATGTTGCAGATATAAAAGAGGTTGCATATGGCATTGTCATTGGTAGCGAAATCGTTAAGCGATTTAAATCTAACACGCGTGAGGAAATCAt                           >  2:97400/1‑141 (MQ=255)
                                                                                                                        ccAATGTGCCAGTTGTCGCAGGATTTGGTATAAGAACACCACAACATGTTGCAGATATAAAAGAGGTTGCAGATGGCATTGTCATTGGTAGCGAAATCGTTAAGCGATTTAAATCTAACACGCGTGAGGAAATCATTAAat                      <  1:225860/141‑1 (MQ=255)
                                                                                                                          aaTGTGCCAGTTGTCGCAGGATTTGGTATAAGAACACCACAACGTGTTGCAGATATAAAAGAGGTTGCAGATGGCATTGTCATTGGTAGCGAAATCGTTAAGCGATTTAAATCTAACACGCGTGAGGAAATCATTAAatat                    <  2:324082/141‑1 (MQ=255)
                                                                                                                                 cAGTTGTCGCAGGATTTGGTATAAGAACACCACAACATGTTGCAGATATAAAAGAGGTTGCAGATGGCATTGTCATTGGTAGCGAAATCGTTAAGCGATTTAAATCTAACACGCGTGAGGAAATCATTAAATATTTACAAt             >  2:153629/1‑141 (MQ=255)
                                                                                                                                            ggATTTGGTATAAGAACACCACAACATGTTGCAGATATAAAAGAGGTTGCAGATGGCATTGTCATTGGTAGCGAAATCGTTAAGCGATTTAAATCTAACACGCGTGAGGAAATCATTAAATATTTACAATCTATCcaacaa  >  2:191457/1‑141 (MQ=255)
                                                                                                                                            ggATTTGGTATAAGAACACCACAACATGTTGCAGATATAAAAGAGGTTGCAGATGGCATTGTCATTGGTAGCGAAATCGTTAAGCGATTTAAATCTAACACGCGTGAGGAAATCATTAAATATTTACAATCTATCcaacaa  >  2:329067/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                            
AAGATATCGTATCCCATGCGGAAGGCTTTATTTATACTGTGACGATGAATGCGACAACAGGGCAAAACGGTGCGTTTCATCCAGAATTAAAACGAAAAATTGAGTCAATTAAAGCGATAGCCAATGTGCCAGTTGTCGCAGGATTTGGTATAAGAACACCACAACATGTTGCAGATATAAAAGAGGTTGCAGATGGCATTGTCATTGGTAGCGAAATCGTTAAGCGATTTAAATCTAACACGCGTGAGGAAATCATTAAATATTTACAATCTATCCAACAA  >  CP000730/1410376‑1410656

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.