breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 44,301 (T)9→8 intergenic (+132/‑359) oprD_1 → / → czcR_2 Porin D/Transcriptional activator protein CzcR
RA 120,983 (T)9→8 intergenic (+280/+92) polA → / ← engB DNA polymerase I/putative GTP‑binding protein EngB
RA 179,484 T→C C316R (TGC→CGC)  CJ019_00182 → hypothetical protein
JC 4,257,335 +GCGGCCGC intergenic (‑281/+7) CJ019_03991 ← / ← CJ019_03994 putative ABC transporter ATP‑binding protein/hypothetical protein
RA 4,759,509 G→A intergenic (+124/‑16) fpvA_3 → / → ligM_KToptimized Ferripyoverdine receptor/sequence generated by OPTIMIZER for KT2440
RA 5,386,500 C→A intergenic (‑509/+1142) CJ019_05070 ← / ← nptII hypothetical protein/neomycin phosphotransferase
RA 5,386,503 C→G intergenic (‑512/+1139) CJ019_05070 ← / ← nptII hypothetical protein/neomycin phosphotransferase
RA 5,386,506 1 bp→GT intergenic (‑515/+1136) CJ019_05070 ← / ← nptII hypothetical protein/neomycin phosphotransferase
RA 5,386,507 +C intergenic (‑516/+1135) CJ019_05070 ← / ← nptII hypothetical protein/neomycin phosphotransferase
RA 5,386,510 2 bp→TA intergenic (‑519/+1131) CJ019_05070 ← / ← nptII hypothetical protein/neomycin phosphotransferase
RA 5,386,513 +AA intergenic (‑522/+1129) CJ019_05070 ← / ← nptII hypothetical protein/neomycin phosphotransferase
RA 5,386,514 T→C intergenic (‑523/+1128) CJ019_05070 ← / ← nptII hypothetical protein/neomycin phosphotransferase
RA 5,386,517 G→C intergenic (‑526/+1125) CJ019_05070 ← / ← nptII hypothetical protein/neomycin phosphotransferase
RA 5,386,519 G→A intergenic (‑528/+1123) CJ019_05070 ← / ← nptII hypothetical protein/neomycin phosphotransferase
RA 5,386,811 C→T intergenic (‑820/+831) CJ019_05070 ← / ← nptII hypothetical protein/neomycin phosphotransferase
MC JC 5,390,395 (CGCAT)2→1 coding (489‑493/1666 nt) ligH → 10‑formyltetrahydrofolate synthetase
RA 5,760,598 (A)6→5 coding (781/834 nt) CJ019_05400 → hypothetical protein

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ ACB143 82143 82330 188 22 [20] [21] 22 trpA Tryptophan synthase alpha chain
* * ÷ ACB143 517190 517305 116 25 [19] [21] 22 tyrS/birA Tyrosine‑‑tRNA ligase/Bifunctional ligase/repressor BirA
* * ÷ ACB143 1119622 1119715 94 23 [20] [19] 45 tdcG_2/gcvP_1 L‑serine dehydratase TdcG/Glycine dehydrogenase (decarboxylating)
* * ÷ ACB143 1149358 1149523 166 22 [20] [21] 22 sasA_4/yihS Adaptive‑response sensory‑kinase SasA/Sulfoquinovose isomerase
* * ÷ ACB143 1914560 1914685 126 26 [17] [21] 22 [ppsC_1]–[CJ019_01830] [ppsC_1],[CJ019_01830]
* * ÷ ACB143 1923406 1923538 133 23 [21] [21] 23 [apeB] [apeB]
* * ÷ ACB143 2467190 2467208 19 30 [0] [13] 31 CJ019_02325/tal hypothetical protein/Transaldolase
* * ÷ ACB143 3046173 3046335 163 25 [19] [20] 22 [rcsC_5]–[degU_2] [rcsC_5],[degU_2]
* * ÷ ACB143 3769361 3769474 114 22 [18] [21] 23 CJ019_03581/CJ019_03582 hypothetical protein/hypothetical protein
* * ÷ ACB143 3944683 3944797 115 22 [20] [21] 22 CJ019_03729/CJ019_03730 hypothetical protein/hypothetical protein
* * ÷ ACB143 4163732 4163875 144 24 [20] [20] 23 katG/leuO_2 Catalase‑peroxidase/HTH‑type transcriptional regulator LeuO
* * ÷ ACB143 4344757 4344833 77 22 [6] [21] 22 nmoA Nitronate monooxygenase
* * ÷ ACB143 4666863 4666996 134 22 [21] [21] 24 [btuB_4] [btuB_4]
* * ÷ ACB143 4732375 4732518 144 23 [20] [21] 22 [CJ019_04468] [CJ019_04468]
* * ÷ ACB143 4801226 4801676 451 23 [20] [17] 27 CJ019_04509/dsbD_2 hypothetical protein/Thiol:disulfide interchange protein DsbD
* * ÷ ACB143 4845672 4845863 192 27 [21] [19] 22 recR/ybaB Recombination protein RecR/Nucleoid‑associated protein YbaB
* * ÷ ACB143 4944218 4944358 141 22 [18] [21] 26 [CJ019_04642] [CJ019_04642]
* * ÷ ACB143 5192928 5193029 102 22 [17] [21] 22 [gltR_4]–[CJ019_04888] [gltR_4],[CJ019_04888]
* * ÷ ACB143 5981569 5981687 119 22 [21] [19] 24 [sasA_19]–[ycaC_2] [sasA_19],[ycaC_2]
* * ÷ ACB143 6003716 6003783 68 23 [19] [21] 22 [CJ019_05617] [CJ019_05617]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? ACB143 2155685 =59 (0.960)52 (0.850) 39/260 0.2 46.9% coding (301/369 nt) tmk_1 Thymidylate kinase
?ACB143 = 2220562 59 (0.960)coding (36/369 nt) tmk_2 Thymidylate kinase
* ? ACB143 2191046 =0 (0.000)107 (1.990)
+TAAGAGATATTTTCAG
49/228 0.0 100% intergenic (‑1/‑313) PP_RS10045/purU_3 LysR family transcriptional regulator/Formyltetrahydrofolate deformylase
?ACB143 = 2191046 NA (NA)intergenic (‑1/‑313) PP_RS10045/purU_3 LysR family transcriptional regulator/Formyltetrahydrofolate deformylase