breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 26 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1431,277,8280TG55.6% 1.2 / 10.5 19intergenic (+20/‑70)pal_2/ompA_1Peptidoglycan‑associated lipoprotein/Outer membrane protein A
*ACB1433,063,5530CG53.8% 5.1 / 16.0 13intergenic (+81/‑273)adh_2/pqqD_2Aldehyde dehydrogenase/PqqA binding protein
*ACB1432,094,8080AC51.2% ‑4.0 / 14.5 43L186R (CTG→CGG) CJ019_01995hypothetical protein
*ACB1435,383,7580CG50.0% 22.7 / 15.8 27intergenic (+528/‑330)CJ019_05064/CJ019_05067putative protein/hypothetical protein
*ACB143675,1240TG46.7% 14.2 / 12.0 30Q400P (CAG→CCG) mcpH_2Methyl‑accepting chemotaxis protein McpH
*ACB1431,175,5520AC46.0% 21.0 / 13.8 37N49T (AAC→ACC) tadAtRNA‑specific adenosine deaminase
*ACB1436,067,3540AC46.0% 36.0 / 11.1 37V162G (GTG→GGG) cheB_5Protein‑glutamatemethylesterase/protein‑glutamin e glutaminase
*ACB143188,0210GC44.4% 19.0 / 14.1 36E960D (GAG→GACCJ019_00185hypothetical protein
*ACB1432,730,1490TG41.5% 27.3 / 13.2 41V1336G (GTG→GGG) CJ019_02576hypothetical protein
*ACB1433,138,3500TG40.0% 36.2 / 16.0 40F240V (TTT→GTT) rbsC_2Ribose import permease protein RbsC
*ACB1433,984,5440GC40.0% 30.3 / 11.0 30G237G (GGC→GGGCJ019_03762hypothetical protein
*ACB1432,076,8650TG38.6% 60.8 / 10.4 57G154G (GGT→GGGCJ019_01978hypothetical protein
*ACB1433,973,7770AC36.5% 61.3 / 13.9 53L161R (CTG→CGG) cobNAerobic cobaltochelatase subunit CobN
*ACB1434,028,4510TA36.1% 41.6 / 13.4 36intergenic (‑120/‑127)CJ019_03802/mcpU_3hypothetical protein/Methyl‑accepting chemotaxis protein McpU
*ACB1432,171,2710GC35.6% 78.5 / 15.4 55V150V (GTG→GTCCJ019_02060IS1182 family transposase ISPpu16
*ACB1434,866,3790TG34.0% 82.2 / 12.3 50N188T (AAC→ACC) aqpZAquaporin Z
*ACB1434,028,4550GC33.3% 40.8 / 14.0 36intergenic (‑124/‑123)CJ019_03802/mcpU_3hypothetical protein/Methyl‑accepting chemotaxis protein McpU
*ACB1434,277,0290CG32.7% 91.6 / 15.3 56F279L (TTC→TTGhbd3‑hydroxybutyryl‑CoA dehydrogenase
*ACB1435,096,4150CG32.6% 58.6 / 10.6 47intergenic (+28/‑162)CJ019_04792/CJ019_04793Putative pterin‑4‑alpha‑carbinolaminedehydratase/hypothetical protein
*ACB1436,043,1960CA31.1% 84.2 / 19.4 61A37S (GCC→TCC) puuD_3Gamma‑glutamyl‑gamma‑aminobutyrate hydrolasePuuD

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? ACB143 3402968 =65 (1.140)11 (0.200) 9/252 3.6 13.5% coding (364/378 nt) CJ019_03221 hypothetical protein
?ACB143 = 3443751 78 (1.420)intergenic (‑131/+258) CJ019_03269/CJ019_03270 hypothetical protein/hypothetical protein