breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 23 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1432,088,2710AC60.0% 2.3 / 14.7 15intergenic (‑265/+52)htpX/alaAProtease HtpX/Glutamate‑pyruvate aminotransferase AlaA
*ACB1431,537,4500TG57.1% 1.3 / 10.3 14W400G (TGG→GGG) mdfAMultidrug transporter MdfA
*ACB1432,088,2740AC53.3% 3.9 / 16.1 15intergenic (‑268/+49)htpX/alaAProtease HtpX/Glutamate‑pyruvate aminotransferase AlaA
*ACB1431,721,8780AC45.0% 18.8 / 14.0 40G95G (GGT→GGGCJ019_01617hypothetical protein
*ACB1432,094,8170TG44.1% 22.0 / 13.8 64Q183P (CAG→CCG) CJ019_01995hypothetical protein
*ACB1435,689,1720AC42.5% 44.9 / 10.7 40P118P (CCA→CCCCJ019_05336hypothetical protein
*ACB1432,094,8080AC38.7% 30.0 / 11.6 63L186R (CTG→CGG) CJ019_01995hypothetical protein
*ACB1434,425,0700GC36.7% 28.0 / 17.4 30intergenic (‑29/‑89)CJ019_04200/CJ019_04201hypothetical protein/hypothetical protein
*ACB1435,383,7580CG35.3% 46.0 / 10.2 34intergenic (+528/‑330)CJ019_05064/CJ019_05067putative protein/hypothetical protein
*ACB1433,273,8780CA30.9% 60.4 / 11.1 42D40E (GAC→GAAfabG_53‑oxoacyl‑[acyl‑carrier‑protein] reductase FabG
*ACB1434,028,4510TA30.9% 63.3 / 14.4 42intergenic (‑120/‑127)CJ019_03802/mcpU_3hypothetical protein/Methyl‑accepting chemotaxis protein McpU
*ACB1432,308,3190CG29.9% 121.2 / 19.4 77A225P (GCA→CCA) fucAL‑fuculose phosphate aldolase
*ACB1431,721,8750GC28.9% 74.1 / 12.0 45G96G (GGC→GGGCJ019_01617hypothetical protein
*ACB1432,113,8950TG26.9% 107.3 / 15.1 67R663R (CGT→CGGCJ019_02007hypothetical protein
*ACB1432,113,8970CG26.2% 131.6 / 12.5 66S664* (TCA→TGA) CJ019_02007hypothetical protein
*ACB1436,043,1960CA25.6% 116.3 / 17.7 78A37S (GCC→TCC) puuD_3Gamma‑glutamyl‑gamma‑aminobutyrate hydrolasePuuD
*ACB1432,348,2510AC25.4% 142.3 / 12.7 72V266G (GTG→GGG) oprJ_1Outer membrane protein OprJ
*ACB1433,047,1930CG23.9% 126.6 / 13.1 67A125P (GCC→CCC) CJ019_02862hypothetical protein
*ACB1433,413,5730GC22.6% 103.0 / 11.1 53intergenic (‑439/‑591)lexA_2/CJ019_03236LexA repressor/hypothetical protein
*ACB1432,127,6290CG21.5% 170.3 / 12.8 83E727Q (GAA→CAA) fadEAcyl‑coenzyme A dehydrogenase

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? ACB143 = 2152216117 (2.050)3 (0.050) 3/256 6.5 2.5% coding (141/1245 nt) fabF_1 3‑oxoacyl‑[acyl‑carrier‑protein] synthase 2
?ACB143 2152220 = 115 (2.040)coding (145/1245 nt) fabF_1 3‑oxoacyl‑[acyl‑carrier‑protein] synthase 2
* ? ACB143 = 491247035 (0.610)3 (0.050) 3/260 6.6 5.9% coding (1553/1557 nt) CJ019_04608 hypothetical protein
?ACB143 4981650 = 61 (1.070)intergenic (+1/+93) ycgR/ycaD_3 Flagellar brake protein YcgR/putative MFS‑type transporter YcaD